Amy2b (NM_001190403) Mouse Tagged ORF Clone

CAT#: MG221812

Amy2b (GFP-tagged) - Mouse amylase 2b (Amy2b) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Amy2b
• Synonyms: Amy-X; Amy2; Amy2-2; Amy2a; mAmy2-1; PA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221812 representing NM_001190403
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTCGTTCTGCTGCTTTCCCTCATTGGGTTCTGCTGGGCTCAATATGACCCACATACTTCAGATG
  GGAGGACTGCTATTGTCCACCTGTTCGAGTGGCGCTGGGTTGATATTGCCAAGGAATGTGAGCGATACTT
  AGCTCCTAAGGGATTTGGAGGGGTGCAGGTCTCTCCACCCAATGAAAATATTGTAATTCATAACCCATCA
  AGGCCTTGGTGGGAAAGATATCAACCAATCAGCTACAAAATTTGCACAAGGTCTGGAAATGAAGATGAAT
  TCAGAGACATGGTGACAAGGTGCAACAATGTTGGTGTCCGTATTTATGTGGATGCTGTCATTAACCACAT
  GTGTGGCTCAGGCAATCCTGCAGGAACAAGCAGTACCTGTGGAAGTTACCTCAATCCAAATAACAGGGAA
  TTCCCAGCAGTTCCATACTCTGCTTGGGACTTTAACGATAATAAATGTAATGGAGAAATTAGTAACTACA
  ATGATGCTTATCAGGTCAGAAATTGTCGTCTGTCTGGCCTTCTGGATCTTGCACTTGAGAAAGATTATGT
  TCGTACCAAGGTTATTGATCTGGGTGGTGAGGCAATTAAAGGTAGTGAGTACTTTGGAAATGGCCGTGTA
  ACAGAATTCAAGTTTGGTGCAAAACTTGGCACAGTTATCCGCAAGTGGAATGGAGAGAAGATGTCCTATT
  TAAAGAACTGGGGAGAAGGTTGGGGTTTGGTGCCTTCTGACAGAGCCCTTGTGTTTGTGGACAACCATGA
  CAATCAGCGAGGACATGGTGCTGGAGGATCATCCATCCTGACATTCTGGGATGCTAGAATGTACAAAATG
  GCTGTTGGATTTATGTTGGCTCATCCTTATGGATTCACAAGAGTAATGTCAAGTTACCGTTGGAATAGAA
  ATTTCCAGAATGGAAAAGATCAGAATGACTGGATTGGACCACCTAATAACAATGGAGTAACAAAAGAAGT
  GACCATTAATGCAGACACTACTTGTGGCAATGACTGGGTCTGTGAACATAGATGGCGTCAAATAAGGAAC
  ATGGTTGCCTTCAGGAATGTAGTCAATGGTCAGCCTTTTGCAAACTGGTGGGATAATAACAGCAACCAAG
  TAGCTTTTAGCAGAGGAAACAGAGGCTTCATTGTCTTTAACAATGATGACTGGGCTTTGTCAGCCACTTT
  ACAGACTGGTCTTCCTGCTGGCACATACTGTGATGTCATCTCTGGAGATAAGGTCGATGGCAATTGCACT
  GGACTTAAAGTAAATGTTGGCAGTGATGGTAAAGCTCACTTTTCCATTAGTAACTCTGCTGAGGACCCAT
  TTATTGCAATCCATGCTGACTCAAAACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221812 representing NM_001190403
  Red=Cloning site Green=Tags(s)
  
  MKFVLLLSLIGFCWAQYDPHTSDGRTAIVHLFEWRWVDIAKECERYLAPKGFGGVQVSPPNENIVIHNPS
  RPWWERYQPISYKICTRSGNEDEFRDMVTRCNNVGVRIYVDAVINHMCGSGNPAGTSSTCGSYLNPNNRE
  FPAVPYSAWDFNDNKCNGEISNYNDAYQVRNCRLSGLLDLALEKDYVRTKVIDLGGEAIKGSEYFGNGRV
  TEFKFGAKLGTVIRKWNGEKMSYLKNWGEGWGLVPSDRALVFVDNHDNQRGHGAGGSSILTFWDARMYKM
  AVGFMLAHPYGFTRVMSSYRWNRNFQNGKDQNDWIGPPNNNGVTKEVTINADTTCGNDWVCEHRWRQIRN
  MVAFRNVVNGQPFANWWDNNSNQVAFSRGNRGFIVFNNDDWALSATLQTGLPAGTYCDVISGDKVDGNCT
  GLKVNVGSDGKAHFSISNSAEDPFIAIHADSKL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190403
• ORF Size: 1359 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001190403.1, NP_001177332.1
• RefSeq Size: 1413
• RefSeq ORF: 1362
• Locus ID: 545562
• Gene Summary: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The mouse genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. At least one mouse strain has a non-functional allele of this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2011]