Ank1 (NM_001110783) Mouse Tagged ORF Clone

CAT#: MG221878

Ank1 (GFP-tagged) - Mouse ankyrin 1 erythroid (Ank1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ank1
• Synonyms: Ank-1; nb; pale
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221878 representing NM_001110783
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGGCGGCCAAACAGCTGAAGAAAATCAAAGACATCGAGGCGCAGGCCCTCCAGGAGCAGAAGG
  AGAAGGAGGAATCCAACAGGAAGCGGCGGAACCGCTCCCGTGACCGCAAGAAGAAGGCCGATGCTGCTAC
  CAGCTTTCTGCGGGCGGCACGCTCGGGGAACCTGGACAAGGCTCTGGATCACCTGCGCAATGGAGTGGAC
  ATTAACACCTGTAACCAGAACGGGTTGAACGGCCTGCATCTGGCCTCCAAAGAAGGCCATGTGAAGATGG
  TGGTTGAACTTCTGCACAAAGAGATCATTCTAGAAACGACAACCAAGAAGGGGAACACTGCTCTGCACAT
  CGCTGCCCTTGCTGGTCAGGATGAGGTGGTCCGGGAGCTGGTCAACTATGGAGCCAATGTCAATGCCCAG
  TCTCAGAAAGGCTTTACTCCCCTGTACATGGCTGCTCAGGAGAACCACTTGGAAGTGGTGAAATTTCTAC
  TGGAGAATGGAGCCAATCAGAATGTAGCCACAGAAGATGGCTTCACCCCACTGGCCGTGGCTCTACAGCA
  GGGTCACGAGAATGTGGTGGCTCACCTCATCAACTATGGGACGAAAGGGAAAGTGCGCCTCCCTGCCCTG
  CACATCGCGGCCCGCAACGATGACACACGGACAGCCGCAGTCCTTCTGCAGAATGACCCCAACCCAGATG
  TGCTTTCCAAGACGGGATTCACACCCCTCCACATCGCAGCTCACTATGAGAACCTCAACGTGGCCCAGTT
  GCTCCTCAACAGGGGAGCCAGCGTCAACTTCACACCTCAGAATGGCATCACCCCACTACACATCGCCTCC
  CGCAGGGGGAACGTGATCATGGTGAGACTCCTGCTGGACCGAGGGGCTCAGATAGAAACGAGGACCAAGG
  ATGAATTGACACCGCTCCACTGTGCAGCTCGGAATGGACACGTGAGAATCTCAGAGATCCTGCTGGACCA
  CGGGGCACCCATCCAAGCCAAAACCAAGAATGGCTTGTCCCCAATCCACATGGCCGCTCAGGGAGACCAC
  CTCGACTGTGTCCGACTTCTATTGCAATACAATGCAGAGATAGACGACATCACCTTGGATCACCTGACTC
  CTCTCCATGTGGCAGCCCACTGTGGCCACCACCGGGTGGCTAAGGTTCTTTTGGATAAAGGGGCCAAGCC
  CAACTCCAGAGCCCTGAATGGTTTTACCCCGTTACACATCGCCTGCAAGAAGAACCACATCCGTGTAATG
  GAGTTGCTGCTGAAGACAGGAGCCTCCATCGACGCGGTCACTGAGTCTGGCCTGACACCTCTCCACGTAG
  CCTCCTTCATGGGACACCTTCCTATTGTGAAGAACTTACTGCAGCGGGGAGCGTCACCCAATGTCTCCAA
  TGTGAAAGTAGAAACCCCCTTGCACATGGCAGCCCGAGCAGGGCATACAGAAGTGGCCAAATATTTGCTC
  CAGAACAAAGCCAAAGCCAACGCCAAGGCCAAGGATGACCAGACACCGCTTCACTGTGCTGCTCGAATCG
  GCCACACAGGCATGGTGAAGCTCCTGCTGGAGAATGGTGCCAGCCCCAATCTGGCTACCACTGCTGGCCA
  CACACCCCTACACACCGCAGCCCGTGAGGGACACGTGGACACAGCCCTGGCCCTGCTGGAGAAGGAGGCA
  TCCCAAGCCTGCATGACCAAGAAAGGATTTACCCCTCTGCACGTGGCTGCTAAGTACGGGAAGGTACGGT
  TGGCGGAGCTGCTGCTGGAACACGATGCACACCCCAATGCAGCTGGGAAGAACGGCTTGACTCCTCTGCA
  TGTGGCCGTCCATCACAACAACCTGGACATTGTCAAACTTCTTCTTCCCCGAGGTGGCTCCCCCCACAGC
  CCTGCCTGGAATGGCTACACTCCTTTGCACATCGCTGCCAAGCAGAACCAGATAGAGGTGGCCCGCAGTC
  TACTGCAGTACGGAGGGTCAGCGAATGCAGAGTCGGTACAAGGCGTGACCCCACTTCACCTGGCTGCCCA
  AGAGGGCCACACAGAAATGGTTGCTCTTCTCCTGTCCAAGCAAGCCAACGGCAACCTGGGGAACAAGAGT
  GGACTCACTCCCCTCCACCTGGTGTCACAAGAAGGCCATGTTCCAGTGGCAGACGTGCTGATCAAACATG
  GCGTCACCGTGGATGCTACCACCCGGATGGGTTACACCCCGCTCCATGTGGCCAGTCATTATGGAAACAT
  CAAGCTGGTGAAGTTCTTGCTACAGCACCAGGCAGATGTCAATGCCAAGACCAAGCTAGGATACAGCCCC
  TTGCACCAGGCAGCTCAACAAGGACACACAGACATTGTGACATTGCTCCTGAAGAATGGTGCTTCTCCAA
  ATGAGGTCAGCTCGAACGGAACCACACCTCTGGCAATAGCCAAACGTTTGGGCTACATCTCTGTAACAGA
  CGTGCTCAAGGTGGTCACAGATGAAACCAGTGTGGTGTTAGTCAGTGACAAACACCGGATGAGCTACCCT
  GAGACGGTGGACGAGATCCTGGACGTGTCTGAAGATGAAGGAACTGCTCACATATCTATAATGGGTGACG
  AACTTGTCGGCTCGAAGGCTGAAAGGCGGGACTCCCGGGATGTGGGTGAGGAGAAAGAGCTGTTGGATTT
  TGTGCCGAAGCTAGACCAAGTGGTGGAGTCTCCAGCCATCCCAAGGATCCCTTGTGTCACACCTGAGACC
  GTTGTGATCCGATCTGAAGATCAGGAACAAGCATCTAAAGAATATGATGAGGATTCCCTCATTCCCAGCA
  GCCCGGCCACAGAGACCTCAGACAACATTAGCCCTGTGGCTAGCCCGGTCCACACAGGGTTCCTGGTGAG
  CTTCATGGTGGATGCCCGAGGGGGATCCATGAGAGGAAGCCGTCATAATGGCCTCAGGGTGGTGATCCCT
  CCCCGGACATGCGCAGCACCCACTCGCATCACCTGCCGCCTCGTTAAGCCGCAGAAGCTGAACACGCCAC
  CCCCACTGGCTGAGGAAGAGGGCCTAGCCAGCAGGATCATTGCCCTGGGACCCACGGGGGCCCAGTTCCT
  TAGCCCTGTGATCGTGGAAATCCCCCATTTTGCCTCCCATGGCCGTGGGGACCGTGAGCTGGTGGTTCTG
  AGGAGTGAAAACGGCTCTGTGTGGAAGGAGCACAAGAGTCGTTATGGGGAAAGCTACCTGGACCAGATCC
  TTAATGGCATGGATGAAGAGCTGGGGAGCCTGGAGGAGCTGGAGAAGAAACGTGTGTGCCGCATCATCAC
  CACCGACTTCCCTCTGTACTTTGTCATCATGTCCCGGCTCTGCCAGGACTACGACACCATCGGTCCTGAA
  GGGGGTTCCCTGAGAAGCAAGCTGGTACCGCTGGTACAGGCAACGTTCCCTGAAAACGCTGTGACCAAGA
  AAGTGAAGCTGGCTCTGCAGGCCCAGCCTGTCCCGGATGAGCTGGTGACCAAGCTCCTGGGTAACCAGGC
  CACATTCAGCCCCATTGTGACCGTGGAGCCGAGGCGTCGGAAGTTCCACCGTCCCATCGGGCTGCGAATC
  CCGCTCCCTCCCTCATGGACGGACAACCCCAGGGACAGTGGGGAGGGAGACACCACCAGCCTGCGCCTAC
  TGTGTAGCGTCATCGGTGGAACTGACCAAGCCCAGTGGGAAGATATAACGGGAACAACCAAACTTATATA
  CGCCAATGAGTGTGCCAACTTTACCACCAATGTCTCAGCCAGGTTCTGGCTGTCAGACTGTCCCCGGACT
  GCGGAGGCTGTGCACTTTGCCACTCTCCTGTACAAAGAGCTCACAGCAGTCCCCTACATGGCTAAGTTTG
  TCATTTTTGCCAAGATGAACGACGCTCGGGAAGGACGGCTCCGTTGCTACTGCATGACGGATGACAAAGT
  GGACAAGACCCTGGAGCAGCATGAAAACTTCGTGGAGGTGGCCCGGAGCAGGGACATAGAGGTTCTGGAA
  GGGATGCCTCTGTTTGCAGAACTCTCTGGGAACTTGGTTCCTGTCAAGAAAGCGGCCCAACAGCGGAGCT
  TCCATTTCCAGTCGTTTCGAGAGAATCGTCTGGCCATCCCCGTGAAGGTGAGGGACAGCAGCCGCGAGCC
  AGGAGGGTTCTTGTCGTTCCTACGAAAGACAATGAAGTACGAGGACACACAGCACATCCTCTGTCACCTG
  AATATCACCATGCCCCCCTGCACCAAGGGCAGTGGAGCAGAAGACAGGAGAAGGACCCTGACACCCCTGA
  CCCTTCGATACAGCATTCTCAGCGAGTCGCGGCTGGGTTTTACCAGTGACACAGACCGTGTCGAAATGAG
  GATGGCTGTCATCAGAGAACACCTCGGCCTAAGCTGGGCAGAGCTGGCCCGGGAGCTGCAGTTCAGCGTG
  GAAGACATCAACCGGATCCGTGTAGAAAACCCTAACTCCTTGTTGGACCAGAGTACAGCCTTGCTGACCC
  TCTGGGTGGACCGTGAAGGCGAAAATGCAAAGATGGAGAATTTGTACACAGCCCTGCGGAACATCGACAG
  GAGTGAGATTGTTAACATGTTGGAGGGCTCCGGCAGGCAGAGCAGAAACCTCAAACCAGAGCGGAGACAT
  GGGGACCGGGAGTACTCATTGTCACCCTCCCAGGTGAATGGTTACTCCTCGCTGCAGGACGAGCTGCTGT
  CCCCCGCCTCCCTGCAGTACGCTCTCCCCTCTCCACTGTGTGCAGACCAGTACTGGAACGAAGTGGCCGT
  CATAGACGCCATCCCCCTGGCGGCTACAGAGCATGACACCATGCTGGAGATGTCTGACATGCAGGTGTGG
  TCTGCGGGCCTCACACCCTCCCTGGTCACTGCTGAGGACTCCTCTCTGGAGTGCAGCAAGGCTGAGGACT
  CTGACGCCATACCAGAGTGGAAGTTGGAAGGGGCACACTCAGAGGACACGCAGGGCCCGGAGCTGGGCTC
  TCAGGACCTGGTGGAGGACGACACAGTGGATTCAGATGCCACAAATGGCCTGGCAGATTTGCTAGGTCAG
  GAGGAAGGTCAGAGGTCAGAAAAGAAGAGGCAGGAAGTCTCAGGCACAGAGCAGGACACGGAGACTGAAG
  TGTCTCTTGTTTCAGGCCAGCAGCGAGTTCACGCCCGAATCACAGACTCACCCTCAGTGAGGCAGGTGCT
  GGACAGAAGCCAGGCCAGAACACTGGACTGGGATAAACAGGGTTCCACAGCGGTACACCCGCAAGAAGCC
  ACACAGAGCTCCTGGCAAGAGGAGGTCACGCAGGGCCCACACTCATTCCAGAGAAGGATCACCACCATCC
  AAGGGCCGGAGCCTGGTGCGCTTCAGGAATACGAGCAGGTGCTGGTGTCTACCAGGGAGCATGTGCAGAG
  GGGGCCACCTGAGACCGGCAGCCCCAAAGCTGGCAAGGAACCTAGCCTGTGGGCACCTGAGAGCGCCTTC
  TCTCAAGAGGTGCAGGGGGATGAGCTTCAGAATATTCCAGGAGAGCAGGTGACGGAGGAACAATTCACAG
  ATGAACAGGGCAACATTGTTACCAAGAAGATCATTCGCAAAGTCGTCCGGCAGGTAGACTCGTCTGGTGC
  CATCGACACCCAGCAGCACGAGGAGGTGGAGCTAAGAGGGAGTGGACTCCAGCCGGACCTGATAGAGGGC
  AGGAAGGGGGCTCAGATAGTGAAGCGGGCCAGCCTGAAAAGGGGCAAGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221878 representing NM_001110783
  Red=Cloning site Green=Tags(s)
  
  MAQAAKQLKKIKDIEAQALQEQKEKEESNRKRRNRSRDRKKKADAATSFLRAARSGNLDKALDHLRNGVD
  INTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQ
  SQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPAL
  HIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIAS
  RRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDH
  LDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVM
  ELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL
  QNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEA
  SQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHS
  PAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKS
  GLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSP
  LHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTDETSVVLVSDKHRMSYP
  ETVDEILDVSEDEGTAHISIMGDELVGSKAERRDSRDVGEEKELLDFVPKLDQVVESPAIPRIPCVTPET
  VVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIP
  PRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVL
  RSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPE
  GGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRI
  PLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLIYANECANFTTNVSARFWLSDCPRT
  AEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLE
  GMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSREPGGFLSFLRKTMKYEDTQHILCHL
  NITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVEMRMAVIREHLGLSWAELARELQFSV
  EDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNIDRSEIVNMLEGSGRQSRNLKPERRH
  GDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEVAVIDAIPLAATEHDTMLEMSDMQVW
  SAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPELGSQDLVEDDTVDSDATNGLADLLGQ
  EEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQVLDRSQARTLDWDKQGSTAVHPQEA
  TQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHVQRGPPETGSPKAGKEPSLWAPESAF
  SQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSSGAIDTQQHEEVELRGSGLQPDLIEG
  RKGAQIVKRASLKRGKQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110783
• ORF Size: 5721 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001110783.1
• RefSeq Size: 8218
• RefSeq ORF: 5724
• Locus ID: 11733
• Gene Summary: Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu7 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils. [UniProtKB/Swiss-Prot Function]