Fga (NM_001111048) Mouse Tagged ORF Clone

CAT#: MG222073

Fga (GFP-tagged) - Mouse fibrinogen alpha chain (Fga) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fga
• Synonyms: Fib
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222073 representing NM_001111048
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCCCTGAGGGTCACCTGCCTCATCTTGAGCGTGGCCAGCACAGTCTGGACTACAGATACCGAAG
  ATAAAGGTGAATTCCTATCAGAAGGAGGAGGAGTACGTGGCCCAAGAGTTGTGGAGAGACATCAGAGTCA
  ATGCAAGGACTCAGACTGGCCCTTCTGCTCTGATGATGACTGGAACCACAAATGCCCTTCAGGCTGCAGG
  ATGAAAGGGTTGATTGACGAAGCCAACCAGGATTTTACAAACAGAATCAACAAGCTCAAAAACTCACTAT
  TTGACTTTCAAAGGAACAACAAGGATTCTAACTCACTGACCAGGAATATCATGGAGTATTTGAGAGGGGA
  CTTCGCGAATGCCAACAACTTTGATAACACTTATGGGCAAGTGTCAGAAGACCTGAGGCGCAGAATTGAG
  ATCCTGAGGCGAAAAGTCATAGAGAAAGCGCAACAGATTCAAGCTCTGCAGAGCAATGTCCGGGCTCAGT
  TGATAGACATGAAGCGCCTGGAGGTGGATATTGATATCAAGATCCGCTCTTGCAAAGGGTCCTGCAGCAG
  GGCTGTAAACCGTGAGATAAATCTACAGGACTATGAAGGTCACCAAAAACAGCTTCAACAGGTCATCGCT
  AAAGAATTGCTTCCTACAAAAGACAGGCAGTACTTGCCAGCACTAAAAATGTCTCCAGTTCCCGACTTGG
  TTCCCGGAAGTTTTAAGAGCCAGCTTCAGGAGGCCCCTCCAGAGTGGAAGGCATTAACAGAAATGCGGCA
  GATGAGAATGGAGCTGGAGAGACCTGGGAAGGATGGGGGTTCGCGAGGAGATTCACCAGGAGACTCGCGG
  GGAGATTCTAGAGGGGACTTTGCAACACGTGGACCAGGGTCAAAGGCAGAAAACCCCACGAACCCTGGAC
  CTGGTGGATCTGGGTATTGGCGTCCTGGGAACTCCGGGTCTGGAAGTGATGGAAATCGGAACCCTGGGAC
  CACGGGGTCTGATGGCACTGGAGACTGGGGTACCGGAAGCCCTAGACCTGGCTCAGACTCTGGGAACTTT
  AGGCCTGCCAACCCTAACTGGGGTGTGTTTTCAGAGTTTGGAGACAGTAGCAGCCCAGCCACAAGAAAAG
  AGTATCACACAGGTAAAGCGGTCACTTCTAAAGGAGATAAAGAGCTCCTGATTGGAAAGGAGAAAGTCAC
  CTCTTCTGGCACAAGCACCACACATCGTTCATGCTCTAAAACCATTACCAAGACTGTCACAGGTCCTGAT
  GGTCGCCGAGAAGTGGTCAAAGAAGTGATCACCTCGGATGATGGCTCAGACTGTGGCGATGCCACCGAGT
  TAGACATATCCCACAGTTTTAGCGGCAGTCTCGACGAACTCTCTGAAAGGCATCCTGACCTTTCTGGGTT
  TTTTGACAACCACTTTGGTTTAATCTCACCTAACTTCAAAGAATTTGGCAGTAAGACCCATTCTGATTCC
  GACATCCTCACAAACATTGAGGACCCCAGCTCCCATGTACCTGAGTTTTCTTCCAGTAGTAAAACCTCAA
  CTGTCAAAAAACAAGTAACCAAGACCTATAAAATGGCAGACGAGGCAGGAAGCGAAGCTCACCGGGAAGG
  AGAAACTCGCAACACTAAGAGGGGCCGTGCCAGAGCTCGCCCAACGAGAGACTGTGATGATGTCCTCCAA
  ACACAAACTTCAGGTGCCCAAAATGGCATTTTCAGTATCAAGCCACCTGGATCCAGTAAGGTTTTTTCTG
  TTTATTGCGATCAAGAGACCAGTTTGGGAGGATGGCTTTTGATCCAGCAAAGAATGGATGGATCGCTGAA
  TTTTAACCGGACCTGGCAAGACTACAAGAGAGGTTTCGGCAGCCTGAATGACAAAGGGGAAGGAGAATTC
  TGGCTAGGCAATGACTACCTCCACTTACTCACTCTGAGGGGCTCAGTTCTCAGGGTTGAATTAGAGGACT
  GGGCTGGAAAAGAGGCTTATGCGGAGTACCACTTCCGGGTAGGCTCTGAGGCAGAGGGCTACGCGCTGCA
  GGTCTCCTCCTACCGGGGCACTGCTGGAGATGCTCTGGTGCAGGGCTCTGTGGAGGAGGGGACAGAATAC
  ACCTCACACAGCAACATGCAGTTCAGTACCTTTGACAGAGATGCCGACCAATGGGAAGAGAACTGTGCAG
  AGGTCTATGGGGGAGGCTGGTGGTACAACAGCTGTCAGGCCGCCAATCTCAATGGCATTTACTACCCCGG
  GGGCACCTACGATCCCAGGAACAACAGCCCCTATGAGATAGAGAACGGGGTGGTCTGGGTTCCCTTCAGA
  GGAGCAGATTATTCCCTCAGGGCCGTTCGGATGAAAATCAGACCGCTGGTGGGACAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222073 representing NM_001111048
  Red=Cloning site Green=Tags(s)
  
  MLSLRVTCLILSVASTVWTTDTEDKGEFLSEGGGVRGPRVVERHQSQCKDSDWPFCSDDDWNHKCPSGCR
  MKGLIDEANQDFTNRINKLKNSLFDFQRNNKDSNSLTRNIMEYLRGDFANANNFDNTYGQVSEDLRRRIE
  ILRRKVIEKAQQIQALQSNVRAQLIDMKRLEVDIDIKIRSCKGSCSRAVNREINLQDYEGHQKQLQQVIA
  KELLPTKDRQYLPALKMSPVPDLVPGSFKSQLQEAPPEWKALTEMRQMRMELERPGKDGGSRGDSPGDSR
  GDSRGDFATRGPGSKAENPTNPGPGGSGYWRPGNSGSGSDGNRNPGTTGSDGTGDWGTGSPRPGSDSGNF
  RPANPNWGVFSEFGDSSSPATRKEYHTGKAVTSKGDKELLIGKEKVTSSGTSTTHRSCSKTITKTVTGPD
  GRREVVKEVITSDDGSDCGDATELDISHSFSGSLDELSERHPDLSGFFDNHFGLISPNFKEFGSKTHSDS
  DILTNIEDPSSHVPEFSSSSKTSTVKKQVTKTYKMADEAGSEAHREGETRNTKRGRARARPTRDCDDVLQ
  TQTSGAQNGIFSIKPPGSSKVFSVYCDQETSLGGWLLIQQRMDGSLNFNRTWQDYKRGFGSLNDKGEGEF
  WLGNDYLHLLTLRGSVLRVELEDWAGKEAYAEYHFRVGSEAEGYALQVSSYRGTAGDALVQGSVEEGTEY
  TSHSNMQFSTFDRDADQWEENCAEVYGGGWWYNSCQAANLNGIYYPGGTYDPRNNSPYEIENGVVWVPFR
  GADYSLRAVRMKIRPLVGQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001111048
• ORF Size: 2367 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001111048.1, NP_001104518.1
• RefSeq Size: 2652
• RefSeq ORF: 2370
• Locus ID: 14161
• Gene Summary: This gene encodes a subunit of the coagulation factor fibrinogen, which is a component of the blood clot. The encoded protein is proteolytically processed by thrombin during the conversion of fibrinogen to fibrin. Mice lacking the encoded protein display bleeding in the peritoneal cavity, skin and soft tissues around joints immediately after birth, and are predisposed to spontaneous fatal abdominal hemorrhage as they grow. Pregnant mice lacking the encoded protein succumb to uterine bleeding during gestation. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Nov 2015]