Arhgef4 (NM_183019) Mouse Tagged ORF Clone

CAT#: MG222131

Arhgef4 (GFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 4 (Arhgef4), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arhgef4
• Synonyms: 9330140K16Rik; Asef; C230030N03Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222131 representing NM_183019
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGACCAGGAGCTGGGCTTCAAAGCTGGGGATGTCATCGAAGTGATGGATGCCACCAACAGGGAAT
  GGTGGTGGGGCCGAGTGGCAGATGGCGAGGGGTGGTTCCCTGCCAGCTTTGTACGGCTGCGCGTGAACCA
  GGATGAGCCCGCGGATGACTATGAGGCCCCGCGGGCCGGGGCTAGAGAGGCCGATGACAGCGGTCCAGAG
  GCTCAGAGCTGCAAGGACCAGATGCGCACAAATGTCATCAATGAGATCCTCAGCACTGAGCGAGACTACA
  TCAAGCACCTTCGCGACATCTGCGAGGGCTATGTCCGCCAGTGCCGCAAACGGGAGGATATGTTCAGTGA
  AGAGCAGCTGCGCACCATCTTTGGGAACATCGAGGACATCTACCGCTGCCAAAAGGCATTTGTGAAGGCT
  TTGGAGCAGAAGTTCAACACGGAGCGGCCACACCTGAGCGAGTTGGGGGCCTGCTTTCTGGAACACCAAG
  CAGACTTCCAGATCTATTCTGAGTACTGCAACAACCACCCCAATGCCTGCGTGGAGCTCTCCCGGCTCAC
  GAAGCTCAGCAAGTACGTGTACTTCTTCGAGGCCTGCCGGCTGCTGCAAAGGATGATTGACATCTCGCTG
  GATGGCTTTCTGCTGACCCCGGTGCAGAAGATCTGCAAGTATCCCCTCCAGCTGGCGGAGCTGCTCAAGT
  ACACACACCCCCAGCACAGGGACTTTAAGAATGTCGAAGCTGCCTTGCACGCCATGAAGAATGTGGCCCA
  GCTCATCAACGAACGGAAACGAAGACTTGAAAACATCGACAAGATTGCTCAGTGGCAGAGCTCCATAGAG
  GACTGGGAGGGGGAAGACCTTCTGGTCAGGAGCTCGGAACTCATCCACTCGGGGGAGCTGACCCGCGTGA
  CACAGCCTCAAGCCAGGAGTCAGCAGAGAATGTTTTTTCTCTTTGACCGCCAGCTCATCTACTGTAAAAA
  GGATCTGCTTCGCCGGGACGTGCTGTACTACAAGGGCCGGCTGGACATGGATGACCTGGAGGTGGTAGAT
  GTGGAAGATGGGAAGGACCGAGACCTCCATGTGAGTGTCAAGAACGCCTTCCGTCTGTACTGTGGTACCA
  CGGGGGACAGCCACCTACTGTGTGCCAGGAAGCCAGAACAGAAGCAGCGCTGGCTGAAGGCCTTTGCCAG
  AGAGAGGGAGCAGGTGCGGCTGGACCAGGAGACAGGCTTCTCCATCACCGAGCTGCAGAGGAAGCAGGCC
  ATGCTGAACGCCAGCAAGCAGCAGGCCACGGGGAAGCCTAAAGCTGTGGGAAGGCCAGGATACCTGACCC
  GCCACAAGCATCCATCATTGCCTGCCAGCCGGCCCCAGCAGCAAGTGTTGGTGCTAGCAGAGCCCAGGCG
  GAAGCCATCTAACTTCTGGCACAGCATCAGCAGACTGGCGCCTTTCCGCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222131 representing NM_183019
  Red=Cloning site Green=Tags(s)
  
  MDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDYEAPRAGAREADDSGPE
  AQSCKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKREDMFSEEQLRTIFGNIEDIYRCQKAFVKA
  LEQKFNTERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQRMIDISL
  DGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKNVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIE
  DWEGEDLLVRSSELIHSGELTRVTQPQARSQQRMFFLFDRQLIYCKKDLLRRDVLYYKGRLDMDDLEVVD
  VEDGKDRDLHVSVKNAFRLYCGTTGDSHLLCARKPEQKQRWLKAFAREREQVRLDQETGFSITELQRKQA
  MLNASKQQATGKPKAVGRPGYLTRHKHPSLPASRPQQQVLVLAEPRRKPSNFWHSISRLAPFRK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183019
• ORF Size: 1452 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_183019.2, NP_898840.2
• RefSeq Size: 2738
• RefSeq ORF: 1455
• Locus ID: 226970