Lig4 (NM_176953) Mouse Tagged ORF Clone

CAT#: MG222165

Lig4 (GFP-tagged) - Mouse ligase IV DNA ATP-dependent (Lig4), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lig4
• Synonyms: 5830471N16Rik; tiny
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222165 representing NM_176953
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCTCACAAACTTCACAAACTGTTGCAGCTCATGTCCCCTTTGCAGACTTATGTTCCACACTAG
  AACGGATACAGAAAGGTAAAGACCGTGCAGAAAAAATCAGGCACTTCAAGGAGTTTTTGGATTCGTGGAG
  AAAATTTCATGATGCCCTTCATAAGAACAGAAAGGACGTTACAGACTCTTTTTACCCTGCAATGAGACTC
  ATTCTCCCCCAGTTAGAAAGAGAGAGGATGGCTTATGGAATCAAAGAAACCATGCTCGCTAAGCTCTACA
  TCGAATTGCTGAACTTACCACGAGAAGGCAAGGATGCCCAGAAGCTCCTCAATTACCGAACCCCCAGTGG
  AGCTCGCACGGATGCTGGGGACTTTGCCATGATTGCATACTTTGTTTTGAAGCCAAGGTGCTTACAGAAA
  GGAAGCTTAACCATACAGCAGGTAAATGAACTCTTAGACTTAGTTGCCAGCAATAACTCTGGCAAAAAAA
  AAGACCTAGTGAAAAAGAGCCTTCTTCAGTTAATAACCCAGAGTTCAGCACTGGAGCAAAAATGGCTGAT
  TCGCATGATTATCAAAGACTTAAAGCTTGGCATCAGTCAGCAAACAATATTTTCCATTTTCCACAATGAT
  GCAGTTGAGTTGCACAACGTCACCACAGATCTGGAAAAGGTCTGCAGGCAGCTGCATGACCCCTCTGTAG
  GGCTTAGTGACATCTCTATCACTCTGTTTTCTGCCTTTAAGCCAATGCTAGCTGCTGTAGCAGACGTGGA
  GCGTGTGGAGAAGGACATGAAGCAGCAGAGTTTCTACATCGAAACTAAGCTTGATGGTGAGCGCATGCAG
  ATGCACAAAGATGGCGCGCTGTACCGGTACTTCTCCAGAAACGGTTACAACTATACCGACCAGTTTGGTG
  AATCTCCACAGGAAGGCTCTCTCACCCCATTTATTCACAATGCGTTCGGGACAGATGTGCAAGCGTGCAT
  CCTTGACGGTGAGATGATGGCCTACAACCCAACAACACAGACTTTCATGCAGAAGGGGGTCAAGTTTGAT
  ATCAAAAGGATGGTGGAAGATTCTGGCCTACAGACTTGTTACTCTGTTTTTGACGTACTGATGGTTAATA
  AGAAGAAGCTAGGGCGCGAGACTCTTAGGAAGAGGTATGAGATCCTTAGTAGCACTTTCACACCCATACA
  AGGCCGCATAGAAATAGTGCAGAAAACTCAAGCTCATACAAAGAAGGAAGTAGTGGATGCATTAAATGAT
  GCCATAGACAAGAGAGAAGAGGGGATCATGGTTAAACACCCTCTGTCCATTTACAAGCCAGACAAAAGAG
  GTGAAGGGTGGCTAAAGATTAAACCAGAGTACGTCAGTGGACTAATGGATGAATTAGACGTCCTAATTGT
  GGGGGGCTACTGGGGTAAAGGTTCACGAGGTGGCATGATGTCTCACTTTTTGTGTGCAGTGGCAGAGACA
  CCACCTCCTGGTGACAGGCCATCTGTATTCCACACTCTGTGCCGTGTTGGGTCTGGTTACACCATGAAAG
  AACTCTATGACCTGGGCTTGAAATTGGCAAAATACTGGAAGCCTTTTCATAAGAAATCTCCACCAAGTAG
  CATTCTGTGTGGTACAGAGAAGCCGGAAGTGTACATAGAGCCTCAGAACTCTGTCATTGTTCAGATCAAG
  GCAGCTGAGATCGTCCCCAGTGACATGTACAAGACTGGCTCCACCCTGCGCTTCCCACGCATCGAAAAGA
  TCAGAGATGACAAAGAGTGGCACGAATGTATGACACTGGGTGACTTGGAGCAGCTGAGGGGGAAAGCATC
  TGGGAAGCTTGCCACGAAACACCTTCATGTAGGTGATGATGATGAACCTAGAGAAAAAAGGCGGAAGCCC
  ATCTCCAAGACGAAGAAAGCCATTAGAATCATTGAACACTTAAAAGCACCCAACCTTTCTAACGTAAACA
  AAGTTTCCAATGTATTTGAAGATGTTGAGTTTTGTGTTATGAGTGGATTAGATGGTTATCCAAAGGCTGA
  CCTAGAGAACAGAATTGCAGAATTCGGTGGTTATATAGTACAGAATCCAGGCCCGGATACATACTGTGTT
  ATTGCAGGTTCTGAGAACGTTAGAGTGAAAAACATTATTTCTTCAGATAAAAATGATGTTGTCAAGCCCG
  AGTGGCTTTTAGAGTGTTTTAAGACAAAAACATGCGTGCCGTGGCAACCTCGCTTTATGATTCACATGTG
  CCCGTCGACAAAGCAGCATTTTGCCCGTGAGTATGACTGCTATGGTGATAGCTATTTTGTTGACACAGAT
  TTGGATCAATTGAAAGAAGTGTTTCTAGGAATTAAACCCAGTGAGCAGCAGACTCCTGAAGAAATGGCCC
  CTGTGATTGCTGACTTAGAATGTCGTTATTCCTGGGACCACTCTCCTCTCAGTATGTTTCGACATTACAC
  CATTTATTTGGACTTGTATGCTGTTATTAATGACTTGAGTTCCAGAATTGAAGCCACGAGATTAGGTATT
  ACAGCCCTTGAGCTGCGGTTTCATGGAGCAAAGGTGGTTTCCTGCTTATCTGAAGGGGTATCTCATGTTA
  TCATTGGGGAGGATCAGAGACGAGTTACTGACTTTAAAATATTCAGAAGAATGCTTAAGAAAAAGTTTAA
  AATCCTGCAAGAAAGTTGGGTGTCCGATTCAGTAGACAAGGGCGAACTGCAGGAGGAAAACCAGTATTTG
  CTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222165 representing NM_176953
  Red=Cloning site Green=Tags(s)
  
  MASSQTSQTVAAHVPFADLCSTLERIQKGKDRAEKIRHFKEFLDSWRKFHDALHKNRKDVTDSFYPAMRL
  ILPQLERERMAYGIKETMLAKLYIELLNLPREGKDAQKLLNYRTPSGARTDAGDFAMIAYFVLKPRCLQK
  GSLTIQQVNELLDLVASNNSGKKKDLVKKSLLQLITQSSALEQKWLIRMIIKDLKLGISQQTIFSIFHND
  AVELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAVADVERVEKDMKQQSFYIETKLDGERMQ
  MHKDGALYRYFSRNGYNYTDQFGESPQEGSLTPFIHNAFGTDVQACILDGEMMAYNPTTQTFMQKGVKFD
  IKRMVEDSGLQTCYSVFDVLMVNKKKLGRETLRKRYEILSSTFTPIQGRIEIVQKTQAHTKKEVVDALND
  AIDKREEGIMVKHPLSIYKPDKRGEGWLKIKPEYVSGLMDELDVLIVGGYWGKGSRGGMMSHFLCAVAET
  PPPGDRPSVFHTLCRVGSGYTMKELYDLGLKLAKYWKPFHKKSPPSSILCGTEKPEVYIEPQNSVIVQIK
  AAEIVPSDMYKTGSTLRFPRIEKIRDDKEWHECMTLGDLEQLRGKASGKLATKHLHVGDDDEPREKRRKP
  ISKTKKAIRIIEHLKAPNLSNVNKVSNVFEDVEFCVMSGLDGYPKADLENRIAEFGGYIVQNPGPDTYCV
  IAGSENVRVKNIISSDKNDVVKPEWLLECFKTKTCVPWQPRFMIHMCPSTKQHFAREYDCYGDSYFVDTD
  LDQLKEVFLGIKPSEQQTPEEMAPVIADLECRYSWDHSPLSMFRHYTIYLDLYAVINDLSSRIEATRLGI
  TALELRFHGAKVVSCLSEGVSHVIIGEDQRRVTDFKIFRRMLKKKFKILQESWVSDSVDKGELQEENQYL
  L
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176953
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_176953.3, NP_795927.2
• RefSeq Size: 4081
• RefSeq ORF: 2736
• Locus ID: 319583