Rapgef4 (NM_019688) Mouse Tagged ORF Clone

CAT#: MG222195

Rapgef4 (GFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 4 (Rapgef4), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rapgef4
• Synonyms: 1300003D15Rik; 5730402K07Rik; 6330581N18Rik; Epac-2; EPAC 2; Epac2; mKIAA4040
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222195 representing NM_019688
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGCTGCGCACGCTGCACACTCTCAGTCCTCGGCCGAGTGGATCGCCTGCCTGGATAAAAGGCCGT
  TGGAGCGATCTAGTGAAGATGTGGACATAATTTTCACGCGGCTGAAAGGAGTTAAAGCTTTTGAGAAATT
  TCACCCAAACCTCCTTCGTCAGATTTGTTTATGCGGTTACTATGAGAACCTGGAAAAAGGAATCACACTG
  TTTCGCCAAGGGGATATTGGAACCAACTGGTATGCTGTCCTGGCTGGGTCTTTGGATGTTAAAGTGTCTG
  AGACCAGCAGTCACCAGGATGCGGTGACCATCTGCACTCTGGGAATTGGGACAGCCTTTGGAGAGTCCAT
  TCTGGATAACACCCCTCGCCATGCAACCATCGTTACCAGGGAGAGCAGCGAACTTCTCCGCATTGAGCAG
  GAGGACTTCAAGGCACTATGGGAGAAATACCGACAGTATATGGCCGGACTTCTGGCTCCTCCCTATGGTG
  TTATGGAAACGGGCTCTAACAATGACAGGATTCCTGACAAGGAGAATGTCCCTTCAGAGAAGATCCTCAG
  AGCTGGAAAAATTTTACGAATTGCCATTCTCTCTCGAGCTCCCCACATGATAAGAGACAGAAAGTACCAC
  CTAAAGACATACAGACAATGCTGTGTTGGGACTGAGCTGGTAGACTGGATGATACAGCAGACATCCTGTG
  TTCACTCGCGGACTCAAGCTGTTGGCATGTGGCAAGTCTTGCTGGAAGATGGTGTCCTCAACCATGTGGA
  CCAGGAGCGCCATTTCCAAGACAAATATTTATTTTATCGATTTCTGGATGACGAGCGTGAGGATGCCCCT
  TTGCCTACTGAGGAAGAGAAGAAGGAGTGTGATGAAGAACTTCAGGACACCATGCTGCTGCTCTCACAGA
  TGGGCCCTGACGCCCACATGAGAATGATCCTGCGAAAACCACCTGGCCAGAGGACTGTGGATGACCTAGA
  GATTATCTACGACGAGCTCCTTCATATTAAAGCCTTATCCCATCTCTCTACCACAGTGAAACGGGAGTTA
  GCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGAACTGTGTTGTTTAACCAGGGGGAAGAAGGTA
  CCTCCTGGTACATCATTCTGAAAGGATCCGTGAATGTAGTCATTTATGGCAAGGGTGTGGTCTGCACCCT
  GCACGAAGGAGATGACTTTGGCAAGTTAGCTCTAGTGAACGATGCTCCAAGAGCTGCCTCCATTGTTCTT
  CGGGAAGATAATTGTCACTTCCTAAGAGTCGACAAGGAAGACTTCAATCGGATTCTGAGGGACGTTGAGG
  CGAATACAGTCAGACTTAAAGAACATGACCAAGATGTCTTGGTACTGGAGAAGGTCCCAGCAGGGAACAG
  AGCTGCTAATCAAGGAAACTCACAGCCTCAGCAAAAGTATACTGTGATGTCAGGAACACCTGAAAAGATT
  TTAGAGCATTTTCTAGAAACAATACGCCTTGAGCCATCGTTGAATGAAGCAACAGATTCGGTTTTAAATG
  ACTTTGTTATGATGCACTGTGTTTTTATGCCAAATACCCAGCTTTGCCCTGCCCTTGTGGCCCATTACCA
  CGCACAGCCTTCTCAAGGTACCGAGCAGGAGAGAATGGATTATGCCCTCAACAACAAGAGGCGGGTCATC
  CGCTTGGTCCTGCAGTGGGCGGCCATGTATGGCGATCTCCTCCAAGAAGATGATGTGGCCATGGCCTTCC
  TGGAGGAGTTCTATGTGTCTGTATCAGATGACGCACGGATGATGGCTGCCTTCAAGGAGCAGCTGCCAGA
  GCTGGAGAAGATTGTCAAGCAAATCTCAGAAGACGCAAAAGCTCCACAGAAGAAGCACAAGGTGCTTTTG
  CAACAGTTCAACACAGGTGACGAGAGGGCCCAGAAGCGTCAGCCTATTCGTGGCTCTGATGAGGTTTTGT
  TCAAGGTCTACTGCATCGACCACACCTATACTACCATTCGTGTGCCGGTAGCTGCCTCGGTGAAGGAAGT
  CATCAGTGCAGTAGCTGACAAACTGGGCTCAGGGGAAGGCCTGATCATCGTCAAGATGAACTCTGGAGGA
  GAAAAGGTGGTGCTGAAATCTAATGATGTTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTG
  CCTGCCCGAGAGAGCAATTCGACTCACTGACTCCCTTGCCGGAACAGGAAGGCCCGACCACTGGGACAGT
  GGGAACATTTGAGCTGATGAGCTCGAAAGACCTGGCGTACCAGATGACAACCTACGATTGGGAACTCTTC
  AACTGTGTGCATGAGCTGGAGCTAATCTACCACACATTTGGAAGGCATAATTTTAAAAAGACCACGGCAA
  ACTTGGATTTGTTCCTGAGGAGGTTTAATGAAATTCAGTTTTGGGTTGTCACTGAGGTCTGCCTTTGTTC
  CCAGCTCAGCAAACGTGTTCAGCTTTTGAAAAAATTTATCAAGATAGCGGCTCACTGCAAGGAGTACAAA
  AATCTAAATTCCTTTTTCGCCATCGTCATGGGACTCAGCAACGTGGCCGTGAGCCGCTTGGCACTAACGT
  GGGAGAAACTGCCGAGCAAGTTTAAGAAGTTCTATGCGGAGTTTGAGAGCTTGATGGATCCTTCCAGAAA
  CCACAGGGCATACAGGCTGACAGCAGCCAAGCTGGAGCCCCCTCTCATCCCTTTCATGCCCTTGCTTATT
  AAAGATATGACATTTACTCATGAGGGGAACAAGACGTTCATTGACAATCTAGTAAACTTTGAAAAAATGC
  GCATGATTGCAAACACTGCCAGAACAGTACGGTACTACAGGAGCCAGCCCTTCAATCCGGATGCCGCTCA
  AGCTAATAAGAACCATCAGGATGTCCGGAGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACT
  TTATCACAGATGTCACACAGATTAGAGCCTCGAAGGCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222195 representing NM_019688
  Red=Cloning site Green=Tags(s)
  
  MVAAHAAHSQSSAEWIACLDKRPLERSSEDVDIIFTRLKGVKAFEKFHPNLLRQICLCGYYENLEKGITL
  FRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTICTLGIGTAFGESILDNTPRHATIVTRESSELLRIEQ
  EDFKALWEKYRQYMAGLLAPPYGVMETGSNNDRIPDKENVPSEKILRAGKILRIAILSRAPHMIRDRKYH
  LKTYRQCCVGTELVDWMIQQTSCVHSRTQAVGMWQVLLEDGVLNHVDQERHFQDKYLFYRFLDDEREDAP
  LPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYDELLHIKALSHLSTTVKREL
  AGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVL
  REDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRAANQGNSQPQQKYTVMSGTPEKI
  LEHFLETIRLEPSLNEATDSVLNDFVMMHCVFMPNTQLCPALVAHYHAQPSQGTEQERMDYALNNKRRVI
  RLVLQWAAMYGDLLQEDDVAMAFLEEFYVSVSDDARMMAAFKEQLPELEKIVKQISEDAKAPQKKHKVLL
  QQFNTGDERAQKRQPIRGSDEVLFKVYCIDHTYTTIRVPVAASVKEVISAVADKLGSGEGLIIVKMNSGG
  EKVVLKSNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTTGTVGTFELMSSKDLAYQMTTYDWELF
  NCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEVCLCSQLSKRVQLLKKFIKIAAHCKEYK
  NLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTAAKLEPPLIPFMPLLI
  KDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRT
  LSQMSHRLEPRRP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019688
• ORF Size: 2979 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019688.1, NP_062662.1
• RefSeq Size: 2982
• RefSeq ORF: 2982
• Locus ID: 56508