Nedd4 (NM_010890) Mouse Tagged ORF Clone

CAT#: MG222243

Nedd4 (GFP-tagged) - Mouse neural precursor cell expressed developmentally down-regulated 4 (Nedd4), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nedd4
• Synonyms: AA959633; AL023035; AU019897; E430025J12Rik; EG639396; Gm7265; KIAA0093; Nedd4-1; Nedd4a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222243 representing NM_010890
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCGGACATGGCAGCCGACGAGTCGGAGGCCCCAGTACTCTCGGAGGACGAGGTATGGGAGTTTT
  GCCTGGATAAGACAGAAGATGGTGGCGGATCCCCCGGAAGTGATGTTACAGACACTTGTGAGCCTCCATG
  TGGATGCTGGGAGTTGAATCCGAATTCCCTGGAAGAGGAGCACGTGCTGTTCACTGCTGATCCGTACCTG
  GAGCTCCACAACGATGACACACGAGTTGTGAGAGTGAAGGTTATAGCTGGCATAGGCCTGGCCAAGAAAG
  ACATCTTGGGAGCCAGTGATCCTTACGTAAGAGTGACATTGTATGACCCGATGAGTGGAATCCTTACCAG
  CGTGCAGACAAAAACTATCAAAAAGTCTTTGAATCCAAAATGGAATGAAGAAATACTGTTCAGGGTCCTT
  CCACAGCGACACCGCATTCTTTTCGAAGTGTTTGATGAAAATCGTTTGACAAGAGATGATTTCCTAGGTC
  AAGTGGATGTCCCTCTCTATCCTTTACCGACTGAAAACCCAAGAATGGAGAGACCATATACATTTAAGGA
  TTTTGTTCTTCATCCAAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGATTAAAAATGACTTATTTA
  CCTAAAAATGGCTCAGAAGATGAAAATGCAGACCAGGCTGAGGAGTTAGAGCCTGGCTGGGTTGTTTTGG
  ACCAACCAGATGCTGCCACTCATTTGCCGCATCCACCAGAACCCTCTCCCCTACCTCCAGGATGGGAAGA
  GAGGCAGGATGTCCTTGGAAGGACCTACTACGTAAACCATGAATCTAGAAGAACACAGTGGAAAAGGCCA
  AGCCCTGACGATGACCTCACGGATGAAGACAATGATGATATGCAGCTGCAAGCGCAGCGAGCATTCACCA
  CCAGGCGGCAGATATCGGAGGATGTGGATGGCCCTGACAACCGGGAGTCCCCTGAGAATTGGGAAATCGT
  ACGAGAAGATGAAAACACCGAGTATAGTGGTCAGGCTGTCCAGTCACCTCCATCGGGTCACATTGATGTG
  CAGACTCACCTTGCAGAAGAGTTTAATACCAGACTTGCCGTGTGTGGAAATCCAGCCACCAGCCAGCCGG
  TTACCAGCTCAAATCATTCCAGCAGAGGAGGCAGCTTGCAGACCTGTATCTTTGAGGAACAGCCTACACT
  TCCTGTGCTTTTGCCTACTTCATCTGGATTGCCACCAGGTTGGGAAGAAAAACAAGATGACAGAGGAAGA
  TCATACTATGTAGACCACAACTCTAAAACCACCACATGGTCCAAGCCCACCATGCAGGATGATCCAAGAT
  CGAAAATCCCTGCTCATCTGAGAGGAAAGACTGACTCCAATGACCTGGGACCCTTACCTCCAGGCTGGGA
  AGAAAGAACCCACACAGATGGGCGAGTCTTCTTCATAAACCACAATATAAAGAAGACCCAGTGGGAAGAT
  CCTCGCCTGCAGAACGTGGCAATCACTGGACCAGCAGTGCCCTACTCCAGAGATTACAAGAGAAAGTACG
  AGTTCTTCAGAAGGAAGCTCAAGAAGCAGACTGACATTCCAAACAAATTTGAAATGAAGCTTCGCCGCGC
  AAACATTCTGGAGGATTCTTACCGGAGGATTATGGGTGTGAAGAGAGCTGACTTGCTCAAGGCCAGACTC
  TGGATTGAGTTTGATGGTGAAAAGGGCCTTGACTATGGAGGGGTTGCCAGAGAGTGGTTCTTCCTCATCT
  CGAAGGAAATGTTCAACCCTTACTACGGCCTGTTTGAATATTCTGCTACGGATAATTACACCCTACAGAT
  AAATCCTAACTCGGGCTTGTGTAATGAAGATCACCTCTCATACTTCAAGTTCATTGGCCGTGTGGCTGGG
  ATGGCAGTTTATCATGGCAAGCTGTTGGATGGGTTTTTCATCCGTCCGTTTTACAAGATGATGCTTCAGA
  AACTGATAACACTGCACGACATGGAGTCCGTGGATAGTGAATATTACAGTTCTCTGCGATGGATTCTTGA
  AAATGACCCGACGGAGCTGGACCTGAGATTTATCATAGATGAAGAACTTTTTGGACAGACACATCAGCAC
  GAACTGAAAACCGGAGGATCAGAGATTGTTGTCACCAATAAGAACAAAAAGGAGTATATCTACCTTGTAA
  TACAATGGCGATTTGTGAACCGTATCCAGAAGCAAATGGCAGCTTTTAAAGAGGGATTTTTTGAACTGAT
  ACCACAGGATCTCATCAAGATATTTGATGAAAATGAGCTAGAGCTTCTCATGTGTGGTCTGGGAGATGTG
  GATGTGAACGACTGGCGGGAACACACAAAATACAAAAATGGCTACAGCATGAACCACCAGGTCATCCACT
  GGTTCTGGAAGGCTGTTTGGATGATGGATTCGGAAAAAAGAATACGCTTACTTCAGTTTGTCACTGGCAC
  ATCCCGTGTGCCGATGAATGGGTTTGCTGAACTCTATGGCTCGAATGGACCACAATCCTTCACAGTGGAA
  CAATGGGGCACCCCTGATAAGCTGCCAAGAGCACACACCTGCTTCAATCGCCTGGACCTGCCACCCTACG
  AATCCTTTGACGAACTCTGGGATAAACTTCAGATGGCAATTGAGAACACACAGGGCTTTGATGGCGTTGA
  T
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222243 representing NM_010890
  Red=Cloning site Green=Tags(s)
  
  MSSDMAADESEAPVLSEDEVWEFCLDKTEDGGGSPGSDVTDTCEPPCGCWELNPNSLEEEHVLFTADPYL
  ELHNDDTRVVRVKVIAGIGLAKKDILGASDPYVRVTLYDPMSGILTSVQTKTIKKSLNPKWNEEILFRVL
  PQRHRILFEVFDENRLTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYL
  PKNGSEDENADQAEELEPGWVVLDQPDAATHLPHPPEPSPLPPGWEERQDVLGRTYYVNHESRRTQWKRP
  SPDDDLTDEDNDDMQLQAQRAFTTRRQISEDVDGPDNRESPENWEIVREDENTEYSGQAVQSPPSGHIDV
  QTHLAEEFNTRLAVCGNPATSQPVTSSNHSSRGGSLQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGR
  SYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQWED
  PRLQNVAITGPAVPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRADLLKARL
  WIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAG
  MAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQH
  ELKTGGSEIVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDV
  DVNDWREHTKYKNGYSMNHQVIHWFWKAVWMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVE
  QWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010890
• ORF Size: 2661 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010890.3, NP_035020.2
• RefSeq Size: 5494
• RefSeq ORF: 2664
• Locus ID: 17999