Dbf4 (NM_001190717) Mouse Tagged ORF Clone

CAT#: MG222368

Dbf4 (GFP-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dbf4
• Synonyms: AA545217; Ask
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222368 representing NM_001190717
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCTCGAGACCATGAGGATCCACAGCAAAGCACCTCTCCCGGGTGGCATCCAAGACAGAAATGAAA
  AGAATCGACCATCCTTGAAATCCCTGAAGGCTGATAATAGACTGGAAAAATCCAAATATAAGCCCCTTTG
  GGGAAAAATATTTTACCTTGACTTGCCTTCTATCACCATATGTGAGAAACTACAAAAAGATATAAAGGAA
  CTCGGAGGGCGAGTTGAAGAATTTCTCAGCAAAGATATCAGTTATTTTGTTTCAAATAAAAAGGAAGCAA
  AGTATGCACAGACATTGGGTCGCGTTTCTCCTGTACCAAGTCCAGAATCTGCATATACTGCAGAAACCAC
  CTCTCCTCATCCCAGCCATGATGGAAGTTCCTTTAAGTCTCAAGACAGAGTATGTTTGAGCCGAGGAAAA
  TTGTTAGCTGAAAAAGCTGTGAAGGATCATGATTTTATTCCTGCTAATAGTATATTATCAAATGCCTTAT
  CATGGGGAGTAAAGATTCTTCATATTGATGACATTCGATACTACATTGAACAGAAGAAGAAAGCACTGAG
  TGCACTCAAGAAATCCAGTGCTTCTGGGAAGGATGCGGGGAAAAAAGCTGGTCCTGGAATACAAAAGACA
  AGAACAGGGAGACTCAAGAAGCCTTTTTTAAAGGTTGAAGATGTGAACCGGTGCTACAGGCCATTTTACC
  TTCAGCTCCCCAGTCTGCCGTGTATAAACTACTTTCTTCAGAAGCCCTGCAGTCCGTTTGATATAGAGAA
  GTCATCCAGCGTCCAGAAACAAGCTCAGCCCAAGCTCAGAATTAATATGGACGGCGATAAATGTGGAACC
  CCAGTTCAACTCCAGTTGAAGGAAAAGAGAAAAAAAGGATATTGTGAATGTTGCTTACAGAAATACGAAG
  ATCTCGAAACTCACCTTCTGAGTGAGAAACACAGGAACTTTGCACAGAGTAACCAGTATCAAGTTGTTGA
  TGATATTGTATCTCAGTTAGTTTTCGATTTTGTGGAATATGGAAGAGACACACCTCAAAAGAAAAGAATA
  AGATACAGTGTCGGGTCCCTTTCTTCTGTTTCTGCAAATGTCCTGAAAAACACTGCACCGAAGGAGAAGC
  CACTCTTGGAGCCCAATTTCCAGAAGGACGTGGGCGAAAGCAGTGGGCACCTGCTCAAGCCGAACTCTCA
  GTATGAAGAAACCCAGAAACCTGAAGAAAAGCATGGGTTTGCTTCAGAGCCCACAACCTATTCTTCAGCT
  GGATTGAAAGGATGTGACAGGAAACCAGTGAGCATGTTCAATGCAAGTGAGCCTGACCCAGAGCAGGAGT
  ATGCACAGCTACCTCTGCGTGACAGCACACCGGAACACCAAGTGACCGAGGGTAGAAATGACGGAGAACA
  GAGAGTAGACCCTGCTCCAGGCGTGTCGCAGTCATGTGGACAAGTTTCTCATCTCAGTACAGAAAGTAAT
  CTACCCCAACCACAGTTAGCTGCAGACATTACACAGCTTTCAGCAAAAGATCTGCAAGAAAAGGGCTTTC
  ATGTTGTGATAGGTCATGCTTCTGACCTGGTGGCATTAAATACATCAAAAGAGCAGCTAACAATGAAGGC
  GAGAACTCCACCCTGTAGTCCCCAGGAGCCTCATGAGTGTGACACTGAGAACATGGAGAATTTGCCTTGT
  GGTAAAATCCAGCGGAAAGTGAGAATGCTATTAGGGCAACAGAAAGCAAATGCTGAACCGAGTGCTGAAT
  TGGATAAAAAGAGAACTGAGTATCTTCCTGCACATGAAGACAGAACTTGTGGGTCACCAGTACAGTCTCT
  ATTGGACCTTTTTCAGACAAGCGAGGAGAAATCAGAATTTTTGGGTTTTACAGGCTACACTGAAAACAGT
  GGAATATGTGATGTTTTAGATATTTGGGAGGAAGAAAATTCAAGTACCCTGTTGTCAACGTTTTTTTCTT
  CCCCTTCAACTTCTGCATTCGTTGGATTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222368 representing NM_001190717
  Red=Cloning site Green=Tags(s)
  
  MNLETMRIHSKAPLPGGIQDRNEKNRPSLKSLKADNRLEKSKYKPLWGKIFYLDLPSITICEKLQKDIKE
  LGGRVEEFLSKDISYFVSNKKEAKYAQTLGRVSPVPSPESAYTAETTSPHPSHDGSSFKSQDRVCLSRGK
  LLAEKAVKDHDFIPANSILSNALSWGVKILHIDDIRYYIEQKKKALSALKKSSASGKDAGKKAGPGIQKT
  RTGRLKKPFLKVEDVNRCYRPFYLQLPSLPCINYFLQKPCSPFDIEKSSSVQKQAQPKLRINMDGDKCGT
  PVQLQLKEKRKKGYCECCLQKYEDLETHLLSEKHRNFAQSNQYQVVDDIVSQLVFDFVEYGRDTPQKKRI
  RYSVGSLSSVSANVLKNTAPKEKPLLEPNFQKDVGESSGHLLKPNSQYEETQKPEEKHGFASEPTTYSSA
  GLKGCDRKPVSMFNASEPDPEQEYAQLPLRDSTPEHQVTEGRNDGEQRVDPAPGVSQSCGQVSHLSTESN
  LPQPQLAADITQLSAKDLQEKGFHVVIGHASDLVALNTSKEQLTMKARTPPCSPQEPHECDTENMENLPC
  GKIQRKVRMLLGQQKANAEPSAELDKKRTEYLPAHEDRTCGSPVQSLLDLFQTSEEKSEFLGFTGYTENS
  GICDVLDIWEEENSSTLLSTFFSSPSTSAFVGF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190717
• ORF Size: 1986 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001190717.1, NP_001177646.1
• RefSeq Size: 2441
• RefSeq ORF: 1989
• Locus ID: 27214