Tia1 (NM_011585) Mouse Tagged ORF Clone

CAT#: MG222478

Tia1 (GFP-tagged) - Mouse cytotoxic granule-associated RNA binding protein 1 (Tia1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tia1
• Synonyms: 2310050N03Rik; AI256674; mTIA-1; TIA-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222478 representing NM_011585
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACGAGATGCCCAAGACTCTATACGTCGGTAACCTTTCTCGAGATGTGACAGAAGCTCTCATCC
  TCCAGCTCTTTAGCCAGATTGGACCTTGTAAAAACTGCAAAATGATTATGGATACAGCAGGAAATGACCC
  ATACTGTTTTGTGGAGTTCCATGAGCACCGTCATGCAGCTGCAGCCCTGGCTGCCATGAATGGGCGGAAG
  ATAATGGGTAAGGAAGTGAAAGTGAATTGGGCAACAACCCCTAGCAGTCAAAAGAAGGATACGAGCAGTA
  GTACCGTTGTCAGCACACAGCGTTCACAAGATCATTTCCATGTGTTTGTTGGTGACCTCAGTCCAGAAAT
  CACAACCGAAGACATCAAAGCAGCGTTTGCACCATTTGGAAGAATTTCAGATGCCCGTGTGGTAAAAGAC
  ATGGCTACCGGGAAGTCTAAGGGATATGGCTTTGTCTCCTTTTTCAACAAATGGGATGCAGAAAATGCCA
  TTCAGCAGATGGGTGGCCAGTGGCTTGGTGGAAGACAAATCAGAACTAACTGGGCAACCCGAAAGCCTCC
  AGCTCCAAAGAGTACATATGAGTCGAACACCAAACAGCTGTCATATGATGAGGTTGTGAGTCAGTCTAGT
  CCCAACAACTGCACTGTGTACTGTGGAGGGGTGACGTCAGGACTGACAGAACAACTAATGCGTCAGACTT
  TTTCTCCATTTGGACAAATAATGGAAATTCGAGTCTTCCCAGATAAAGGATACTCATTTGTTCGGTTCAG
  TTCCCATGAAAGTGCAGCACATGCGATTGTTTCTGTTAATGGTACTACCATTGAAGGGCATGTGGTGAAA
  TGTTATTGGGGCAAAGAGACTCTTGATATGATAAACCCTGTGCAACAGCAAAATCAAATTGGATATCCAC
  CAACATATGGCCAGTGGGGCCAGTGGTATGGAAATGCACAACAGATTGGCCAGTATGTGCCTAACGGTTG
  GCAAGTACCTGCCTATGGAGTGTATGGCCAGCCATGGAGCCAGCAGGGGTTCAATCAGACCCAGTCTTCT
  GCACCGTGGATGGGACCCAATTACAGTGTGCCACCACCTCAAGGGCAGAATGGCAGCATGTTGCCTAGTC
  AGCCTGCTGGGTATCGAGTGGCCGGGTATGAAACCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222478 representing NM_011585
  Red=Cloning site Green=Tags(s)
  
  MEDEMPKTLYVGNLSRDVTEALILQLFSQIGPCKNCKMIMDTAGNDPYCFVEFHEHRHAAAALAAMNGRK
  IMGKEVKVNWATTPSSQKKDTSSSTVVSTQRSQDHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKD
  MATGKSKGYGFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVSQSS
  PNNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFSSHESAAHAIVSVNGTTIEGHVVK
  CYWGKETLDMINPVQQQNQIGYPPTYGQWGQWYGNAQQIGQYVPNGWQVPAYGVYGQPWSQQGFNQTQSS
  APWMGPNYSVPPPQGQNGSMLPSQPAGYRVAGYETQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011585
• ORF Size: 1158 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011585.4, NP_035715.1
• RefSeq Size: 4477
• RefSeq ORF: 1161
• Locus ID: 21841