Med1 (NM_013634) Mouse Tagged ORF Clone

CAT#: MG222482

Med1 (GFP-tagged) - Mouse mediator complex subunit 1 (Med1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Med1
• Synonyms: AI480703; CRSP210; DRIP205; l11Jus15; PBP; Pparbp; TRAP220; TRIP-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222482 representing NM_013634
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTCCCTCCTGGAACGGCTCCATGCAAAATTTAACCAGAACAGACCTTGGAGTGAAACCATTAAGC
  TTGTGCGTCAAGTAATGGAGAAGAGGGTCGTAATGAGTTCTGGAGGGCATCAGCATTTGGTCAGCTGTTT
  GGAGACATTGCAGAAGGCTCTCAAAGTAACATCTTTGCCAGCAATGACTGATCGTTTGGAATCTATAGCC
  AGACAGAATGGACTGGGCTCTCACCTCAGTGCCAGTGGCACTGAGTGTTACATCACGTCAGATATGTTCT
  ATGTGGAAGTGCAGTTAGATCCTGCAGGACAGCTTTGTGATGTCAAAGTGGCTCACCATGGGGAGAATCC
  TGTGAGCTGTCCAGAGCTTGTACAGCAGTTAAGGGAAAAGAATTTTGAGGAATTTTCCAAGCATCTTAAG
  GGTCTTGTTAATCTGTATAATCTCCCAGGGGACAACAAACTGAAGACTAAAATGTATCTGGCTCTCCAAT
  CCTTAGAACAGGACCTTTCTAAAATGGCTATTATGTACTGGAAGGCAACCAACGCCGCTCCCTTGGATAA
  GATTCTTCATGGAAGTGTTGGTTATCTCACCCCGCGGAGTGGGGGTCATTTAATGAATATGAAATACTAT
  GCCTCTCCATCTGACCTGCTGGATGATAAGACTGCCTCTCCTATCATTTTGCATGAAAAGAATGTTCCTC
  GGTCTTTGGGAATGAATGCCTCAGTGACAATTGAAGGAACCTCTGCTATGTACAAACTCCCAATTGCCCC
  ATTAATTATGGGGTCACACCCAGCTGACAACAAATGGACCCCTTCTTTCTCCGCAGTCACTAGTGCCAAC
  AGTGTTGATCTTCCTGCGTGTTTCTTCTTGAAATTTCCCCAGCCAATTCCAGTATCTAAAGCATTTGTTC
  AGAAACTGCAAAATTGCACAGGAATCCCGTTGTTTGAGACTCCGCCCACTTACCTGCCCCTGTATGAACT
  CATCACTCAGTTTGAGCTGTCAAAGGATCCTGACCCTTTACCTTTGAATCACAACATGCGATTTTACGCT
  GCTCTTCCAGGTCAGCAGCACTGCTATTTTCTCAATAAAGATGCTCCTCTTCCTGATGGTCAGAGCCTGC
  AGGGAACACTGGTCAGCAAAATCACCTTCCAGCACCCTGGCCGAGTTCCTCTTATCTTGAATATGATCAG
  ACACCAAGTGGCCTATAACACTCTAATTGGAAGCTGTGTCAAAAGAACTATTTTAAAAGAAGATTCTCCT
  GGGCTCCTCCAGTTTGAAGTGTGTCCTCTCTCAGAATCTCGCTTCAGTGTATCTTTTCAGCACCCTGTGA
  ATGACTCCCTTGTGTGTGTGGTGATGGATGTGCAAGACTCAACACATGTGAGCTGTAAACTCTACAAGGG
  GCTGTCAGATGCACTAATCTGTACAGACGACTTCATTGCCAAAGTTGTTCAAAGATGTATGTCCATTCCT
  GTGACGATGAGGGCTATTCGGAGGAAGGCTGAAACCATACAGGCTGACACCCCAGCACTGTCTCTCATTG
  CAGAGACAGTTGAAGACATGGTGAAAAAGAACCTGCCCCCGGCTAGCAGCCCAGGGTATGGCATGACCAC
  AGGCAACAACCCAATGAGTGGTACCACTACACCAACCAACACCTTTCCGGGGGGTCCCATTACCACCTTG
  TTTAATATGAGCATGAGCATCAAAGATCGGCATGAGTCGGTGGGCCATGGGGAGGACTTCAGCAAGGTGT
  CTCAGAACCCAATTCTTACCAGTTTGTTGCAAATCACAGGGAACGGGGGGTCTACCATTGGCTCGAGTCC
  GACCCCTCCTCATCACACGCCGCCACCTGTCTCTTCGATGGCCGGCAACACCAAGAACCACCCGATGCTC
  ATGAACCTTCTTAAAGATAACCCTGCCCAGGATTTCTCAACCCTTTATGGAAGCAGCCCTTTAGAAAGGC
  AGAACTCCTCTTCCGGATCACCCCGGATGGAAATGTGCTCGGGGAGCAACAAGGCCAAGAAGAAGAAGTC
  GTCAAGAGTCCCACCTGACAAACCCAAGCACCAGACTGAAGACGATTTCCAGAGGGAGCTCTTTTCCATG
  GATGTCGACTCACAGAACCCTATGTTTGACGTCAGCATGACCGCTGACGCGCTGGATACACCTCATATCA
  CCCCAGCTCCAAGCCAGTGTAGCACTCCCCCAGCAACGTACCCACAGCCAGTGTCTCACCCCCAGCCCAG
  TATTCAGAGGATGGTCCGACTGTCCAGTTCAGACAGCATTGGCCCAGATGTAACTGATATTCTTTCAGAT
  ATTGCCGAAGAAGCTTCAAAGCTTCCCAGCACGAGTGATGACTGCCCACCAATTGGCACCCCTGTTCGAG
  ATTCCTCAAGTTCTGGGCATTCTCAGAGTGCCCTCTTTGATTCTGATGTCTTTCAAACTAATAATAATGA
  AAATCCATACACTGATCCAGCTGACCTTATTGCAGATGCTGCTGGAAGCCCCAATAGTGATTCTCCTACC
  AATCATTTTTTCCCTGATGGAGTAGATTTCAATCCTGATTTGTTGAACAGCCAAAGCCAAAGTGGTTTTG
  GAGAGGAGTATTTTGATGAAAGTAGTCAGAGTGGGGATAATGATGATTTCAAAGGATTTGCATCTCAGGC
  ATTAAATACATTGGGGATGCCAATGCTTGGAGGTGACAATGGGGAGCCAAAATTTAAGGGCAGCAGCCAG
  GCTGACACGGTGGACTTCAGTATTATATCAGTAGCCGGTAAGGCTTTGGGTGCTGCAGATCTGATGGAGC
  ACCACAGTGGGAGTCAGAGTCCTTTACTGACCACTGGAGAATTAGGGAAAGAAAAAACTCAAAAGAGGGT
  GAAGGAAGGCAACGGCACAGGTGCTAGCAGTGGATCAGGTCCAGGGTCAGACAGCAAGCCAGGCAAGCGC
  AGCCGCACTCCCTCCAATGATGGGAAGAGCAAGGATAAGCCTCCAAAGCGGAAGAAGGCAGACACTGAGG
  GGAAGTCCCCATCTCACAGTTCTTCTAATAGACCTTTCACCCCACCTACCAGCACGGGTGGGTCCAAATC
  CCCAGGCAGTTCAGGACGATCTCAGACGCCCCCAGGTGTTGCCACCCCGCCCATTCCCAAGATTACCATT
  CAGATTCCTAAAGGGACAGTGATGGTGGGCAAGCCCTCCTCTCACAGTCAGTACACTAGCAGTGGTTCTG
  TGTCTTCCTCTGGCAGCAAAAGCCACCATAGTCATTCTTCCTCCTCCTCTTCCTTAGCTTCTGCTTCCAC
  CTCAGGCAAGGTGAAAAGCAGTAAATCTGAAGGCTCATCAAGTTCCAAGCTCAGTGGCAGTATGTATGCT
  AGCCAAGGGTCTTCTGGATCCAGCCAGTCCAAAAATTCATCTCAGACTGGGGGGAAGCCAGGCTCCTCTC
  CCATTACCAAACATGGACTGAGCAGTGGGTCCAGCAGTACCAAGATGAAACCTCAAGGCAAGCCATCCTC
  CCTTATGAACCCTTCTATAAGTAAGCCAAACATATCCCCTTCCCATTCAAGGCCTCCCGGAGGCTCAGAT
  AAGCTTGCCTCTCCAATGAAGCCTGTTCCTGGAACCCCCCCATCCTCTAAAGCCAAGTCCCCTATCAGTT
  CAGGTTCCAGTGGTTCTCATGTGTCAGGAACTAGTTCAAGCTCTGGTATGAAGTCATCTTCAGGGTCAGC
  ATCCTCAGGCTCAGTGTCTCAAAAAACCCCTCCAGCATCTAATTCTTGTACACCATCTTCCTCTTCGTTT
  TCCTCAAGTGGTTCTTCCATGTCATCCTCTCAGAATCAACATGGCAGTTCCAAAGGGAAATCTCCCAGTA
  GGAATAAGAAGCCTTCCTTGACAGCTGTCATAGATAAATTGAAGCATGGGGTGGTTACCAGTGGGCCTGG
  GGGTGAGGATCCAATAGACAGTCAGATGGGCGCAAGCACAAATTCTTCTAACCATCCCATGTCCTCCAAA
  CATAACACGTCAGGAGGGGAGTTCCAGAGCAAACGTGAGAAAAGTGATAAAGACAAATCCAAGGTCTCTG
  CTTCTGGGGGGTCAGTGGATTCCTCTAAGAAGACTTCAGAGTCAAAAAATGTGGGGAGCACGGGGGTGGC
  AAAAATCATTATCAGCAAGCACGACGGAGGCTCCCCGAGCATCAAAGCCAAAGTGACGCTACAGAAACCT
  GGAGAAAGTGGTGGAGATGGGCTCAGGCCACAGATAGCCTCATCAAAGAACTATGGCTCTCCACTTATCA
  GTGGTTCCACTCCAAAGCACGAACGGGGTTCTCCCAGCCACAGTAAGTCGCCAGCATATACACCACAGAA
  TGTGGACAGTGAAAGTGAGTCAGGCTCCTCCATAGCAGAGAGATCCTACCAGAACAGTCCCAGCTCAGAG
  GATGGTATCCGACCACTTCCAGAGTACAGCACTGAGAAGCATAAGAAGCACAAAAAGGAAAAGAAGAAAG
  TCAGAGACAAAGACAGAGACAAGAAGAAGTCTCACAGCATGAAGCCAGAGAACTGGTCGAAATCCCCCAT
  TTCTTCAGATCCGACGGCGTCTGTGACAAATAACCCTATCTTATCTGCAGACAGGCCTTCTAGGCTCAGC
  CCTGACTTCATGATTGGGGAGGAAGATGATGATCTCATGGATGTGGCCCTGATTGGCAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222482 representing NM_013634
  Red=Cloning site Green=Tags(s)
  
  MSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIA
  RQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFEEFSKHLK
  GLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAAPLDKILHGSVGYLTPRSGGHLMNMKYY
  ASPSDLLDDKTASPIILHEKNVPRSLGMNASVTIEGTSAMYKLPIAPLIMGSHPADNKWTPSFSAVTSAN
  SVDLPACFFLKFPQPIPVSKAFVQKLQNCTGIPLFETPPTYLPLYELITQFELSKDPDPLPLNHNMRFYA
  ALPGQQHCYFLNKDAPLPDGQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVKRTILKEDSP
  GLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIP
  VTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTL
  FNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPML
  MNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNKAKKKKSSRVPPDKPKHQTEDDFQRELFSM
  DVDSQNPMFDVSMTADALDTPHITPAPSQCSTPPATYPQPVSHPQPSIQRMVRLSSSDSIGPDVTDILSD
  IAEEASKLPSTSDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPNSDSPT
  NHFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGMPMLGGDNGEPKFKGSSQ
  ADTVDFSIISVAGKALGAADLMEHHSGSQSPLLTTGELGKEKTQKRVKEGNGTGASSGSGPGSDSKPGKR
  SRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSSGRSQTPPGVATPPIPKITI
  QIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSLASASTSGKVKSSKSEGSSSSKLSGSMYA
  SQGSSGSSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSISKPNISPSHSRPPGGSD
  KLASPMKPVPGTPPSSKAKSPISSGSSGSHVSGTSSSSGMKSSSGSASSGSVSQKTPPASNSCTPSSSSF
  SSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPIDSQMGASTNSSNHPMSSK
  HNTSGGEFQSKREKSDKDKSKVSASGGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKP
  GESGGDGLRPQIASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAERSYQNSPSSE
  DGIRPLPEYSTEKHKKHKKEKKKVRDKDRDKKKSHSMKPENWSKSPISSDPTASVTNNPILSADRPSRLS
  PDFMIGEEDDDLMDVALIGN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013634
• ORF Size: 4680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013634.2, NP_038662.2
• RefSeq Size: 6383
• RefSeq ORF: 4683
• Locus ID: 19014