Adam22 (NM_001098225) Mouse Tagged ORF Clone

CAT#: MG222780

Adam22 (GFP-tagged) - Mouse a disintegrin and metallopeptidase domain 22 (Adam22) transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adam22
• Synonyms: 2900022I03Rik; AI854032; MDC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222780 representing NM_001098225
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCAGCGGCGGCCGCGTCCTTCTGGCTGCTCTGCGTCTTGGGGACCTGCCCCCTGGCGCGCTGCG
  GTCGGGCAGGAGTCGCCTCACTGAAAGGACTGGAGAGGGGGAAAGAAAACCGCTTCCTGGAGCGCCAGAG
  CATCATACCACTGCGCCTCATCTATCGCTTGGGCGGCGAGGATGAAACTCAGCACAACCAGCTCGACACG
  CGGGTGCGGGGCGACCCTGGCGGACCGCAGCTGACGCATGTGGACAAGGCAAGCTTCCGGGTGGACGCCT
  TTGGAACCTCTTTCGTTCTCGACGTCCTGCTGAACCACGAGTTGCTGTCGTCAGGTTATGTAGAGAGACA
  GATTGAACATGGAGGCAAGGTCGTGGAGAACAAAGGAGGAGAACACTGTTATTACCAGGGCCAGATTCGA
  GGAAACCCTGTCTCATTTGTTGCCCTATCAACATGCCATGGACTCCATGGGATGTTCTATGATGGAAACC
  ACACATATCTCATTGAGCCAGAAGAAAATGAGAAGTCTCAAGAGTCGTCTCATTGTCACTCCGTGTACAA
  ATCCAGACAGTTTGAATTTCCCTTGGATGACCTTCCGTCTGAATTTCAACGCGTAAACATTACTCCCCCT
  CAGTTTATTTTGAAGCCAAGACTAAAGAGGAGAAAAAGACAGCTTCTTCGATTTCCTCGGAATGTAGAGG
  AGGAAACCAAATACATCGAACTGATGATTGTGAATGATCATCTCATGTTTAAAAAGCACCGGCTTTCTGT
  TGTATATACTAATACCTATGCGAAGTCTGTGGTGAACATGGCAGATGTGATCTATAAAGACCAACTGAAA
  ACTAGGATTGTATTGGTTGCCATGGAAACCTGGGCTGCTGACAACAAGTTTGCCATATCTGAAAACCCAC
  TGATTACCCTTCGTGAGTTCATGAAATACAGGAGGGATTTTATCAAGGAGAAGGCTGATGCAGTTCATCT
  TTTCTCGGGCAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCGCTGCTG
  AGAGGAGGAGGCGTGAATGAATTTGGAAAAACGGATTTAATGGCAGTTACCCTCGCTCAGTCACTAGCCC
  ACAATGTCGGTATAATCTCTGACAAAAGAAAACTAGCAAGTGGTGAGTGTAAATGTGAGGACACCTGGTC
  CGGGTGCATAATGGGAGACACCGGCTACTATCTCCCGAAAAAGTTCACTCAGTGCAATGTCGAGGAATAT
  CATGACTTCCTGAATAGTGGTGGAGGTGCCTGCCTGTTCAACAAGCCTTCCAAGCTTCTTGATCCTCCTG
  AGTGTGGAAACGGCTTCATTGAAACCGGAGAGGAGTGTGATTGTGGCACCCCTGCAGAATGTGCCCTTGA
  AGGGGCAGAGTGTTGTAAGAAATGCACCTTGACCCAGGATTCTCAATGCAGTGATGGCCTTTGTTGTAAA
  AAGTGCAAGTTTCAGCCTCTGGGGACCGTGTGCCGAGAGGCAGTGAACGACTGTGACATTCGTGAAATAT
  GTTCAGGAAATTCGAGCCAGTGTGCCCCCAATGTGCATAAAATGGATGGATATTCGTGTGATGGTACTCA
  GGGAATTTGCTTTGGAGGAAGATGTAAAACCAGAGATAGACAATGCAAATACATCTGGGGGCAAAAGGTA
  ACAGCATCTGACAGGTACTGCTATGAGAAACTGAACATTGAGGGGACGGAGAAGGGTAACTGTGGGAAAG
  ACAAGGACACGTGGACACAGTGCAACAAACGGGATGTACTTTGTGGTTACCTCCTGTGCACAAACATTGG
  CAACATCCCAAGGCTCGGGGAGCTTGATGGTGAGATCACATCCACATTAGTTGTGCAGCAAGGAAGAACA
  TTAAACTGCAGTGGCGCGCATGTTAAACTTGAAGAAGATGTGGATCTTGGCTATGTGGAAGATGGGACGC
  CCTGTGGCCCTCAGATGATGTGCTTGGAACACAGGTGTCTTCCTGTGGCTTCCTTCAACTTCAGTACTTG
  CTCCAGCAGTAAAGCAGGCACCGTGTGCTCAGGAAATGGAGTTTGCAGCAATGAGCTCAAGTGTGTGTGC
  AACAGGCACTGGACAGGGGCTGACTGCGGCACCCACTTTCCTCACAATGATGATGCAAAGACTGGCATCA
  CCCTGTCTGGCAATGGTGTTGCTGGCACTAATATCATAATAGGAATAATTGCCGGCACCATTTTAGTGCT
  GGCCCTCATCTTAGGAATAACTGCCTGGGGTTATAAAAACTACCGAGAACAGAGGTCAAATGGACTCTCT
  CATTCTTGGAGTGAAAGGATTCCAGACACAAAACATATTTCAGACATCTGTGAAAATGGGAGACCTCGCA
  GTAACTCCTGGCAAGGTAACATGGGAGGCAACAAAAAGAAAATCAGAGGGAAAAGATTTAGACCTCGATC
  TAACTCAACTGAGACCCTGTCACCTGCCAAGTCTCCTTCTTCATCAACTGGGTCTATTGCCTCCAGCAGA
  AAATACCCGTACCCTATGCCTCCGCTTCCAGACGAGGGGAAGACAGCGGGCCGACAGAGCGCCAGGCTAT
  GGGAGACATCCATT
  
  CTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222780 representing NM_001098225
  Red=Cloning site Green=Tags(s)
  
  MQAAAAASFWLLCVLGTCPLARCGRAGVASLKGLERGKENRFLERQSIIPLRLIYRLGGEDETQHNQLDT
  RVRGDPGGPQLTHVDKASFRVDAFGTSFVLDVLLNHELLSSGYVERQIEHGGKVVENKGGEHCYYQGQIR
  GNPVSFVALSTCHGLHGMFYDGNHTYLIEPEENEKSQESSHCHSVYKSRQFEFPLDDLPSEFQRVNITPP
  QFILKPRLKRRKRQLLRFPRNVEEETKYIELMIVNDHLMFKKHRLSVVYTNTYAKSVVNMADVIYKDQLK
  TRIVLVAMETWAADNKFAISENPLITLREFMKYRRDFIKEKADAVHLFSGSQFESSRSGAAYIGGICSLL
  RGGGVNEFGKTDLMAVTLAQSLAHNVGIISDKRKLASGECKCEDTWSGCIMGDTGYYLPKKFTQCNVEEY
  HDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECALEGAECCKKCTLTQDSQCSDGLCCK
  KCKFQPLGTVCREAVNDCDIREICSGNSSQCAPNVHKMDGYSCDGTQGICFGGRCKTRDRQCKYIWGQKV
  TASDRYCYEKLNIEGTEKGNCGKDKDTWTQCNKRDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQGRT
  LNCSGAHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCSSSKAGTVCSGNGVCSNELKCVC
  NRHWTGADCGTHFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWGYKNYREQRSNGLS
  HSWSERIPDTKHISDICENGRPRSNSWQGNMGGNKKKIRGKRFRPRSNSTETLSPAKSPSSSTGSIASSR
  KYPYPMPPLPDEGKTAGRQSARLWETSI
  
  LE - GFP Tag - V
• Restriction Sites: SgfI-XhoI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098225
• ORF Size: 2604 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001098225.1, NP_001091695.1
• RefSeq Size: 9068
• RefSeq ORF: 2607
• Locus ID: 11496
• Gene Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. The protein encoded by this gene is believed to lack metalloproteinase activity due to the lack of a critical catalytic motif. Mice lacking the encoded protein exhibit severe ataxia, hypomyelination and premature death. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016]