Chrnd (NM_021600) Mouse Tagged ORF Clone

CAT#: MG222857

Chrnd (GFP-tagged) - Mouse cholinergic receptor nicotinic delta polypeptide (Chrnd), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chrnd
• Synonyms: Achr-4; Acrd; L10076
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222857 representing NM_021600
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGGCCTGTGCTCACACTGGGGCTGCTGGCTGCCCTTGTTGTGTGTGCCCTCCCAGGCAGTTGGG
  GCCTGAATGAGGAACAAAGGCTGATCCAGCACCTATTCAATGAGAAGGGCTACGACAAAGACCTGCGGCC
  GGTGGCTCGAAAGGAGGACAAAGTGGATGTCGCCCTAAGTCTCACCCTCTCCAACCTCATCTCCCTGAAA
  GAAGTGGAGGAGACCCTCACCACCAACGTGTGGATAGATCATGCCTGGGTAGACAGCCGTCTACAGTGGG
  ATGCCAACGACTTCGGGAACATCACTGTCCTGCGCCTGCCCCCTGACATGGTGTGGCTACCAGAGATTGT
  ACTGGAGAACAACAATGATGGCTCGTTCCAGATTTCTTATGCCTGCAATGTGCTTGTCTATGACTCGGGC
  TATGTGACCTGGCTGCCGCCCGCCATCTTCCGCTCCTCCTGCCCCATCTCAGTCACCTACTTCCCTTTCG
  ACTGGCAGAACTGCTCCCTCAAATTCAGTTCACTCAAGTATACAGCCAAGGAGATCACACTTAGCCTGAA
  GCAGGAGGAGGAAAACAACCGCTCTTACCCCATCGAGTGGATCATCATTGACCCTGAAGGTTTCACAGAG
  AACGGTGAGTGGGAGATAGTGCATCGGGCAGCCAAGCTCAATGTGGACCCCAGTGTCCCGATGGACAGCA
  CCAACCACCAAGATGTCACCTTCTACCTTATCATCCGCCGCAAGCCCCTCTTCTACATCATCAACATTCT
  GGTACCCTGCGTGCTCATCTCCTTCATGATCAACCTAGTCTTCTACCTGCCAGGCGACTGTGGAGAGAAG
  ACCTCCGTGGCCATCTCAGTGCTCCTGGCCCAATCTGTCTTCCTGCTGCTTATCTCCAAGAGGCTGCCGG
  CCACTTCCATGGCCATCCCCTTGGTAGGCAAGTTCCTGCTTTTCGGCATGGTGCTGGTCACCATGGTGGT
  GGTGATCTGTGTCATCGTACTGAACATCCACTTCCGGACGCCCAGCACCCATGTGCTGTCTGAGGGAGTC
  AAGAAGTTCTTCCTGGAGACCCTGCCCAAGCTCCTGCACATGTCCCGCCCAGCAGAGGAAGACCCAGGCC
  CCAGGGCTCTCATCCGGAGAAGCAGCTCTCTGGGATACATCTGCAAAGCAGAGGAGTATTTCTCACTTAA
  GTCCCGCAGTGACCTCATGTTTGAGAAGCAATCAGAGCGGCATGGGCTGGCCCGGCGCCTCACCACAGCC
  CGCAGGCCTCCAGCAAGCTCTGAGCAGGTCCAACAGGAGCTCTTCAATGAGATGAAGCCAGCTGTGGATG
  GGGCAAACTTCATCGTCAACCATATGAGAGACCAAAACAGTTACAATGAGGAGAAGGACAACTGGAACCA
  GGTGGCCCGCACAGTGGACCGTCTCTGCCTGTTTGTGGTGACACCCGTCATGGTGGTGGGCACAGCCTGG
  ATCTTCCTGCAGGGTGTCTACAACCAGCCCCCACTCCAGCCTTTCCCTGGGGACCCCTTCTCCTATAGCG
  AACAGGACAAGCGCTTCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222857 representing NM_021600
  Red=Cloning site Green=Tags(s)
  
  MAGPVLTLGLLAALVVCALPGSWGLNEEQRLIQHLFNEKGYDKDLRPVARKEDKVDVALSLTLSNLISLK
  EVEETLTTNVWIDHAWVDSRLQWDANDFGNITVLRLPPDMVWLPEIVLENNNDGSFQISYACNVLVYDSG
  YVTWLPPAIFRSSCPISVTYFPFDWQNCSLKFSSLKYTAKEITLSLKQEEENNRSYPIEWIIIDPEGFTE
  NGEWEIVHRAAKLNVDPSVPMDSTNHQDVTFYLIIRRKPLFYIINILVPCVLISFMINLVFYLPGDCGEK
  TSVAISVLLAQSVFLLLISKRLPATSMAIPLVGKFLLFGMVLVTMVVVICVIVLNIHFRTPSTHVLSEGV
  KKFFLETLPKLLHMSRPAEEDPGPRALIRRSSSLGYICKAEEYFSLKSRSDLMFEKQSERHGLARRLTTA
  RRPPASSEQVQQELFNEMKPAVDGANFIVNHMRDQNSYNEEKDNWNQVARTVDRLCLFVVTPVMVVGTAW
  IFLQGVYNQPPLQPFPGDPFSYSEQDKRFI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021600
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021600.2, NP_067611.2
• RefSeq Size: 2948
• RefSeq ORF: 1563
• Locus ID: 11447
• Gene Summary: This gene encodes the delta subunit of the muscle-derived nicotinic acetylcholine receptor, a pentameric neurotransmitter receptor and member of the ligand-gated ion channel superfamily. The delta subunit together with the alpha subunit forms the ligand-binding site. [provided by RefSeq, Nov 2012]