Sptan1 (NM_001177668) Mouse Tagged ORF Clone

CAT#: MG222896

Sptan1 (GFP-tagged) - Mouse spectrin alpha 2 (Spna2) transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sptan1
• Synonyms: 2610027H02Rik; Spna-2; Spna2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222896 representing NM_001177668
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCAAGTGGGGTCAAAGTGCTGGAAACAGCCGAGGACATCCAGGAGAGACGACAGCAAGTCCTGG
  ATCGGTACCACCGCTTCAAGGAGCTCTCTACCTTACGGCGTCAGAAGCTGGAGGATTCCTATCGGTTCCA
  GTTTTTTCAAAGAGATGCTGAGGAGTTGGAGAAGTGGATTCAGGAGAAGCTTCAAGTTGCATCTGATGAG
  AACTACAAAGACCCAACCAACTTGCAGGGAAAGCTGCAGAAGCACCAAGCCTTTGAAGCTGAAGTACAGG
  CTAACTCAGGAGCCATTGTGAAGCTCGACGAGACAGGAAACTTGATGATTTCTGAAGGGCACTTTGCATC
  TGAAACCATCCGGACACGTTTAATGGAGCTGCACCGGCAGTGGGAACTGCTTTTGGAGAAGATGCGGGAG
  AAAGGAATCAAACTGCTGCAGGCACAGAAGCTGGTGCAGTATTTGCGGGAGTGTGAGGATGTAATGGACT
  GGATCAATGACAAGGAAGCAATTGTGACTTCTGAGGAGCTGGGCCAGGACCTGGAGCATGTAGAGGTGCT
  ACAGAAGAAGTTTGAAGAGTTTCAGACTGATCTGGCTGCTCATGAAGAAAGAGTTAATGAAGTGAGCCAG
  TTTGCTGCCAAACTCATCCAGGAGCAGCACCCGGAAGAGGAGCTGATCAAGACCAAGCAGGATGAGGTGA
  ATGCAGCATGGCAGCGACTGAAAGGCCTGGCTCTTCAAAGGCAGGGCAAGCTGTTCGGTGCTGCTGAGGT
  CCAGCGCTTTAACAGGGATGTAGATGAGACCATTGGTTGGATTAAGGAGAAAGAGCAGTTAATGGCCTCT
  GATGACTTCGGCAGAGACTTAGCAAGTGTTCAAGCTCTGCTTCGAAAGCATGAGGGTCTGGAGAGAGATC
  TTGCTGCTCTAGAGGACAAGGTGAAAGCCCTGTGTGCTGAGGCTGACCGCCTGCAACAGTCACACCCTCT
  GAGTGCCAGCCAGATCCAGGTGAAGCGAGAGGAGCTGATTACCAACTGGGAACAGATCCGAACTCTGGCA
  GCAGAGAGACATGCACGGCTTGATGACTCGTACAGGCTTCAGCGCTTTCTTGCTGACTTCCGTAACCTCA
  CAAGCTGGGTAACTGAAATGAAAGCCCTCATCAATGCAGATGAACTGGCCAATGATGTGGCTGGTGCTGA
  AGCCCTGCTGGACAGGCATCAAGAGCACAAGGGTGAGATCGACGCTCATGAAGATAGCTTTAAGTCTGCA
  GATGAGTCTGGGCAAGCTCTGCTTGCGGCTAGTCACTATGCCTCAGATGAAGTGAGGGAGAAGCTGAGCA
  TCCTCTCCGAGGAGAGAACTGCCCTGCTGGAGCTGTGGGAGCTTCGGAGGCAGCAGTATGAGCAGTGCAT
  GGACTTGCAGCTCTTCTACCGTGACACTGAGCAGGTGGACAACTGGATGAGCAAGCAGGAGGCATTCCTG
  CTAAATGAAGACTTGGGTGACTCCTTAGACAGTGTGGAAGCTCTTTTGAAGAAGCATGAGGACTTTGAAA
  AATCTCTCAGTGCCCAGGAAGAAAAAATCACAGCCCTTGATGAATTTGCAACCAAACTCATCCAAAATAA
  CCACTACGCAATGGAAGATGTAGCGACTCGACGAGATGCTCTCCTGAGCCGCCGCAATGCCCTTCATGAG
  CGAGCCATGCATCGCCGGGCACAGCTGGCTGATTCCTTCCATCTGCAGCAGTTCTTCCGTGATTCTGATG
  AGCTCAAAAGTTGGGTCAACGAGAAGATGAAAACGGCCACCGATGAAGCTTATAAAGATCCATCCAACCT
  GCAAGGGAAAGTCCAAAAGCATCAGGCTTTTGAGGCTGAGCTTTCAGCAAACCAGAGCCGGATTGATGCC
  CTTGAGAAAGCTGGGCAGAAGCTAATAGATGTCAACCACTATGCCAAGGAAGAAGTAGCAGCTCGTATGA
  ATGAGGTCATCAGTTTGTGGAAGAAACTTCTAGAGGCCACAGAACTGAAAGGAATAAAGCTCCGAGAAGC
  CAACCAGCAGCAACAATTTAATCGAAATGTTGAAGACATTGAATTGTGGCTGTATGAAGTAGAAGGTCAT
  TTGGCTTCAGATGATTATGGTAAAGACCTCACTAATGTCCAGAACCTCCAGAAGAAGCATGCTCTGCTAG
  AGGCGGATGTTGCTGCTCACCAGGATCGAATTGATGGCATCACAATTCAGGCCCGCCAGTTCCAAGATGC
  TGGCCATTTTGATGCCGAAAATATTAAGAAGAAGCAAGAGGCCCTTGTGGCTCGCTATGAGGCTCTTAAG
  GAACCCATGGTGGCCCGGAAACAGAAGCTGGCAGATTCTCTTCGTCTGCAGCAGCTCTTCCGGGATGTGG
  AGGACGAAGAAACCTGGATTCGAGAAAAGGAGCCTATTGCTGCATCCACTAACAGAGGCAAAGATCTTAT
  TGGAGTCCAGAATCTGCTAAAGAAACACCAAGCTTTGCAAGCAGAGATTGCTGGGCATGAACCTCGAATC
  AAAGCAGTGACACAAAAGGGAAATGCCATGGTGGAGGAAGGCCATTTTGCTGCAGAGGATGTGAAGGCCA
  AACTGAGTGAGCTCAACCAGAAGTGGGAGGCTCTGAAAGCCAAAGCCTCCCAGCGGAGGCAGGATCTAGA
  GGACTCGCTGCAGGCCCAGCAGTACTTTGCCGATGCCAATGAGGCTGAGTCCTGGATGCGGGAGAAGGAG
  CCCATTGTGGGCAGTACGGACTATGGGAAGGATGAAGACTCTGCTGAGGCTCTACTCAAGAAGCATGAAG
  CTTTGATGTCCGATCTCAGCGCCTATGGCAGCAGCATCCAGGCTCTGCGAGAACAGGCCCAGTCATGCCG
  GCAACAAGTGGCCCCCATGGATGATGAGACTGGGAAGGAGCTAGTCTTGGCTCTCTATGACTATCAAGAG
  AAGAGTCCTCGTGAGGTCACCATGAAGAAAGGGGATATCCTCACCTTGCTCAACAGCACAAACAAGGATT
  GGTGGAAAGTGGAAGTGAATGACCGGCAGGGTTTCGTGCCAGCTGCATATGTGAAGAAGCTGGACCCCGC
  CCAGTCAGCCTCGAGGGAGAACCTCCTAGAAGAGCAGGGCAGCATTGCTCTGCGGCAGGGGCAAATTGAC
  GACCAATATCAGTCTCTGCTGGAACTGGGTGAGAAGAGAAAAGGCATGTTGGAGAAGAGTTGCAAGAAGT
  TCATGTTGTTCCGGGAAGCAAACGAGTTACAGCAGTGGATCACTGAGAAGGAGGCTGCTCTAACCAATGA
  GGAGGTTGGCGCTGACTTGGAGCAGGTTGAGGTGCTGCAGAAGAAGTTTGATGACTTCCAGAAGGATCTG
  AAAGCCAATGAGTCTCGGCTGAAGGACATTAACAAAGTAGCAGAAGACCTGGAGTCTGAAGGTCTGATGG
  CAGAGGAAGTGCAAGCTGTGCAGCAGCAGGAGGTGTATGGTGCGATGCCCAGGGATGAAGCAGATTCCAA
  GACGGCCTCCCCATGGAAGTCTGCTCGACTGATGGTCCATACAGTGGCTACCTTCAACTCCATCAAGGAG
  CTGAATGAGCGTTGGCGGTCCCTGCAACAGCTGGCTGAGGAACGTAGCCAGCTCTTGGGCAGTGCACACG
  AAGTACAGAGGTTTCACAGAGATGCTGATGAAACTAAAGAATGGATTGAAGAGAAGAATCAGGCTCTGAA
  CACAGACAACTATGGCCATGATTTAGCTAGTGTCCAGGCCCTGCAGCGCAAACATGAAGGCTTTGAGAGG
  GACCTTGCAGCTCTTGGTGACAAGGTGAATTCCCTTGGGGAAACAGCCCAAAGGCTGATCCAGTCCCACC
  CTGAATCTGCAGAGGACTTAAAGGAAAAGTGCACAGAGTTAAACCAGGCCTGGACCAGCCTAGGGAAGCG
  TGCAGACCAGCGCAAGGCCAAATTGGGTGACTCCCATGATCTGCAGCGCTTCCTTAGCGATTTCCGGGAC
  CTCATGTCTTGGATCAATGGAATACGAGGGTTGGTATCTTCAGATGAACTAGCCAAGGACGTCACTGGAG
  CTGAGGCTTTGCTGGAGCGACACCAGGAACACCGGACAGAAATTGATGCCAGGGCTGGCACTTTCCAGGC
  ATTTGAGCAGTTTGGGCAGCAGCTGTTAGCTCACGGGCACTATGCCAGCCCGGAGATCAAAGAGAAACTT
  GATATTCTTGACCAGGAGCGCACAGACCTGGAGAAGGCCTGGGTCCAGCGCAGGATGATGCTGGACCACT
  GCCTGGAGTTGCAGCTGTTCCATCGGGACTGTGAACAAGCAGAAAACTGGATGGCTGCCCGGGAGGCCTT
  CCTAAACACAGAAGACAAAGGAGACTCGCTGGACAGTGTGGAGGCACTGATCAAAAAACATGAAGACTTT
  GACAAAGCTATCAATGTCCAGGAAGAAAAGATAGCTGCCCTGCAGGCCTTTGCCGACCAGCTCATTGCCG
  TTGACCACTATGCCAAGGGAGACATTGCAAACCGACGCAATGAGGTCCTTGACCGGTGGCGCCGCCTAAA
  AGCCCAGATGATTGAAAAAAGGTCAAAACTTGGAGAATCTCAAACACTTCAGCAGTTCAGCCGGGATGTG
  GATGAGATTGAAGCCTGGATCAGTGAGAAGTTACAGACAGCCAGTGATGAGTCATACAAGGACCCCACCA
  ACATCCAGCTTTCCAAGCTGTTGAGCAAGCACCAGAAGCACCAAGCCTTTGAGGCAGAACTGCACGCCAA
  TGCTGACCGAATCCGTGGAGTTATCGACATGGGCAACTCCCTCATTGAGCGTGGGGCCTGTGCTGGCAGT
  GAGGATGCTGTCAAGGCCCGCCTGGCTGCCCTTGCAGACCAGTGGCAGTTCCTGGTGCAGAAATCAGCTG
  AGAAGAGCCAGAAGCTAAAAGAGGCAAATAAGCAGCAGAACTTCAACACTGGAATCAAAGACTTTGACTT
  CTGGCTCTCTGAGGTGGAGGCTCTCCTGGCATCGGAAGATTATGGCAAAGACCTGGCTTCTGTGAACAAC
  TTGCTGAAAAAGCATCAGCTGCTAGAGGCAGACATCTCGGCCCACGAGGATCGTCTGAAGGACCTGAACA
  GCCAGGCTGACAGCCTGATGACTAGCAGTGCCTTCGACACCTCCCAAGTGAAAGAGAAGCGGGACACCAT
  CAATGGACGCTTCCAGAAGATCAAGAGCATGGCCACCTCCCGAAGAGCGAAACTGAGCGAGTCCCACCGC
  CTGCACCAGTTCTTCCGGGACATGGACGACGAGGAGTCCTGGATCAAGGAGAAGAAGTTGTTAGTGAGCT
  CTGAGGACTATGGCAGAGACCTCACTGGTGTTCAGAATCTGAGGAAGAAACACAAGCGGCTAGAAGCTGA
  ACTGGCTGCACATGAACCCGCCATTCAGGGTGTCCTGGACACTGGGAAGAAGCTGTCTGATGACAACACC
  ATCGGGCAGGAGGAGATCCAGCAACGGCTTGCACAGTTTGTGGAGCACTGGAAGGAACTAAAGCAGCTGG
  CGGCTGCGCGGGGCCAGCGGCTAGAGGAGTCCTTGGAGTATCAGCAGTTTGTAGCCAACGTGGAAGAGGA
  GGAGGCTTGGATCAATGAGAAGATGACTCTGGTGGCCAGCGAAGACTATGGAGACACTCTTGCTGCTATC
  CAGGGCTTACTGAAGAAACACGAAGCTTTTGAGACAGACTTCACTGTCCACAAGGATCGGGTGAATGATG
  TCTGTACTAATGGACAAGACCTCATTAAGAAGAACAATCACCATGAGGAGAACATCTCTTCAAAGATGAA
  GGGTCTGAATGGAAAGGTGTCTGATCTGGAGAAAGCAGCAGCTCAGAGGAAAGCAAAGCTGGATGAGAAC
  TCAGCCTTCCTTCAGTTCAACTGGAAGGCTGACGTGGTAGAGTCCTGGATTGGTGAAAAGGAGAACAGCT
  TGAAAACAGATGACTATGGCCGAGATCTGTCTTCTGTCCAAACTCTGCTCACAAAACAGGAGACATTTGA
  TGCTGGCCTGCAGGCCTTCCAGCAGGAGGGTATTGCCAATATCACTGCCCTCAAAGACCAGCTGCTAGCT
  GCCAAGCACATTCAGTCTAAGGCCATCGAGGCCCGACATGCCTCCCTCATGAAGAGGTGGACCCAGCTGT
  TGGCCAATTCAGCTACCCGGAAGAAGAAGTTGCTGGAGGCCCAGAGTCATTTCCGTAAGGTAGAAGACCT
  CTTCCTGACCTTTGCCAAAAAGGCATCGGCTTTCAATAGCTGGTTTGAGAATGCAGAGGAAGACCTCACA
  GACCCAGTGCGCTGCAACTCTCTGGAAGAAATCAAAGCCCTTCGAGAGGCTCACGATGCCTTCCGCTCAT
  CCCTCAGCTCTGCACAGGCTGACTTCAACCAGCTAGCCGAGCTGGACCGCCAGATCAAGAGTTTCCGGGT
  GGCCTCCAACCCCTATACCTGGTTCACGATGGAGGCCCTGGAAGAGACCTGGAGAAACCTCCAGAAGATC
  ATTAAGGAACGAGAACTGGAGCTGCAGAAGGAACAGCGGCGGCAGGAGGAGAATGACAAGCTGCGCCAAG
  AATTTGCCCAGCATGCCAACGCCTTCCACCAGTGGATCCAGGAAACAAGAACGTATCTCCTCGACGGGTC
  CTGCATGGTGGAAGAGTCGGGAACTCTGGAATCTCAGCTTGAAGCTACCAAACGCAAGCACCAGGAGATT
  CGAGCCATGAGAAGTCAGCTGAAGAAGATCGAGGACCTGGGGGCTGCCATGGAGGAAGCCCTCATCCTGG
  ACAACAAGTACACGGAGCACAGCACTGTGGGCCTGGCCCAGCAGTGGGACCAGTTAGACCAGCTGGGCAT
  GCGCATGCAGCACAACCTGGAGCAGCAGATCCAGGCCAGGAACACAACAGGAGTGACTGAGGAGGCCCTC
  AAGGAATTCAGCATGATGTTCAAACACTTCGACAAGGACAAGTCTGGCCGGCTGAACCATCAGGAGTTCA
  AATCCTGTCTTCGCTCCCTGGGCTATGATCTGCCAATGGTTGAGGAAGGAGAGCCTGATCCTGAGTTTGA
  GGCAATTCTGGACACTGTTGATCCCAACAGGGATGGCCACGTCTCCCTGCAAGAATACATGGCTTTCATG
  ATCAGCCGCGAAACGGAGAATGTCAAGTCCAGTGAAGAGATCGAGAGTGCTTTCCGGGCCCTCAGCTCTG
  AGGGCAAGCCTTATGTGACCAAGGAGGAGCTCTACCAGAACCTGACCCGGGAACAAGCTGACTATTGTGT
  CTCCCACATGAAGCCCTATGTGGATGGCAAGGGCCGAGAACTTCCAACTGCCTTCGACTACGTCGAGTTC
  ACCCGCTCACTCTTTGTGAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222896 representing NM_001177668
  Red=Cloning site Green=Tags(s)
  
  MDPSGVKVLETAEDIQERRQQVLDRYHRFKELSTLRRQKLEDSYRFQFFQRDAEELEKWIQEKLQVASDE
  NYKDPTNLQGKLQKHQAFEAEVQANSGAIVKLDETGNLMISEGHFASETIRTRLMELHRQWELLLEKMRE
  KGIKLLQAQKLVQYLRECEDVMDWINDKEAIVTSEELGQDLEHVEVLQKKFEEFQTDLAAHEERVNEVSQ
  FAAKLIQEQHPEEELIKTKQDEVNAAWQRLKGLALQRQGKLFGAAEVQRFNRDVDETIGWIKEKEQLMAS
  DDFGRDLASVQALLRKHEGLERDLAALEDKVKALCAEADRLQQSHPLSASQIQVKREELITNWEQIRTLA
  AERHARLDDSYRLQRFLADFRNLTSWVTEMKALINADELANDVAGAEALLDRHQEHKGEIDAHEDSFKSA
  DESGQALLAASHYASDEVREKLSILSEERTALLELWELRRQQYEQCMDLQLFYRDTEQVDNWMSKQEAFL
  LNEDLGDSLDSVEALLKKHEDFEKSLSAQEEKITALDEFATKLIQNNHYAMEDVATRRDALLSRRNALHE
  RAMHRRAQLADSFHLQQFFRDSDELKSWVNEKMKTATDEAYKDPSNLQGKVQKHQAFEAELSANQSRIDA
  LEKAGQKLIDVNHYAKEEVAARMNEVISLWKKLLEATELKGIKLREANQQQQFNRNVEDIELWLYEVEGH
  LASDDYGKDLTNVQNLQKKHALLEADVAAHQDRIDGITIQARQFQDAGHFDAENIKKKQEALVARYEALK
  EPMVARKQKLADSLRLQQLFRDVEDEETWIREKEPIAASTNRGKDLIGVQNLLKKHQALQAEIAGHEPRI
  KAVTQKGNAMVEEGHFAAEDVKAKLSELNQKWEALKAKASQRRQDLEDSLQAQQYFADANEAESWMREKE
  PIVGSTDYGKDEDSAEALLKKHEALMSDLSAYGSSIQALREQAQSCRQQVAPMDDETGKELVLALYDYQE
  KSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQGQID
  DQYQSLLELGEKRKGMLEKSCKKFMLFREANELQQWITEKEAALTNEEVGADLEQVEVLQKKFDDFQKDL
  KANESRLKDINKVAEDLESEGLMAEEVQAVQQQEVYGAMPRDEADSKTASPWKSARLMVHTVATFNSIKE
  LNERWRSLQQLAEERSQLLGSAHEVQRFHRDADETKEWIEEKNQALNTDNYGHDLASVQALQRKHEGFER
  DLAALGDKVNSLGETAQRLIQSHPESAEDLKEKCTELNQAWTSLGKRADQRKAKLGDSHDLQRFLSDFRD
  LMSWINGIRGLVSSDELAKDVTGAEALLERHQEHRTEIDARAGTFQAFEQFGQQLLAHGHYASPEIKEKL
  DILDQERTDLEKAWVQRRMMLDHCLELQLFHRDCEQAENWMAAREAFLNTEDKGDSLDSVEALIKKHEDF
  DKAINVQEEKIAALQAFADQLIAVDHYAKGDIANRRNEVLDRWRRLKAQMIEKRSKLGESQTLQQFSRDV
  DEIEAWISEKLQTASDESYKDPTNIQLSKLLSKHQKHQAFEAELHANADRIRGVIDMGNSLIERGACAGS
  EDAVKARLAALADQWQFLVQKSAEKSQKLKEANKQQNFNTGIKDFDFWLSEVEALLASEDYGKDLASVNN
  LLKKHQLLEADISAHEDRLKDLNSQADSLMTSSAFDTSQVKEKRDTINGRFQKIKSMATSRRAKLSESHR
  LHQFFRDMDDEESWIKEKKLLVSSEDYGRDLTGVQNLRKKHKRLEAELAAHEPAIQGVLDTGKKLSDDNT
  IGQEEIQQRLAQFVEHWKELKQLAAARGQRLEESLEYQQFVANVEEEEAWINEKMTLVASEDYGDTLAAI
  QGLLKKHEAFETDFTVHKDRVNDVCTNGQDLIKKNNHHEENISSKMKGLNGKVSDLEKAAAQRKAKLDEN
  SAFLQFNWKADVVESWIGEKENSLKTDDYGRDLSSVQTLLTKQETFDAGLQAFQQEGIANITALKDQLLA
  AKHIQSKAIEARHASLMKRWTQLLANSATRKKKLLEAQSHFRKVEDLFLTFAKKASAFNSWFENAEEDLT
  DPVRCNSLEEIKALREAHDAFRSSLSSAQADFNQLAELDRQIKSFRVASNPYTWFTMEALEETWRNLQKI
  IKERELELQKEQRRQEENDKLRQEFAQHANAFHQWIQETRTYLLDGSCMVEESGTLESQLEATKRKHQEI
  RAMRSQLKKIEDLGAAMEEALILDNKYTEHSTVGLAQQWDQLDQLGMRMQHNLEQQIQARNTTGVTEEAL
  KEFSMMFKHFDKDKSGRLNHQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAFM
  ISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSHMKPYVDGKGRELPTAFDYVEF
  TRSLFVN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177668
• ORF Size: 7371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001177668.1, NP_001171139.1
• RefSeq Size: 7979
• RefSeq ORF: 7374
• Locus ID: 20740