Tle3 (NM_009389) Mouse Tagged ORF Clone

CAT#: MG223072

Tle3 (GFP-tagged) - Mouse transducin-like enhancer of split 3 homolog of Drosophila E(spl) (Tle3) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tle3
• Synonyms: 2610103N05Rik; ESG; Grg3a; Grg3b; mKIAA1547
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223072 representing NM_009389
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCCGCAAGGCAGACATCCGGCACCCCATCAACCCGGGCAGCCGGGATTTAAATTCACTGTGGCCG
  AGTCCTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTA
  TGACAAGCTGGCTAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGC
  TTGAATATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATCCTAGCCCAGATCATGC
  CTTTTTTGTCACAGGAGCATCAGCAGCAAGTGGCGCAGGCTGTGGAACGCGCCAAGCAGGTCACCATGAC
  GGAGTTGAACGCCATCATCGGGCAGCAGCAACTCCAGGCCCAGCACCTCTCCCATGCCACGCATGGTCCC
  CCGGTCCAGCTGCCACCCCACCCGTCAGGCCTCCAGCCTCCTGGGATTCCCCCAGTGACAGGAAGCAGCT
  CTGGGTTGCTGGCACTTGGTGCCCTGGGAAGTCAAGCTCACTTGGCGGTGAAGGATGAGAAGAACCACCA
  TGAACTGGATCACAGAGAGAGAGAGTCCAGCACGAACAATTCCGTGTCACCCTCTGAAAGCCTCCGGGCC
  AGTGAGAAGCACCGGGGCTCTGCAGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAAGAGAAAGACA
  GCCTCAGCAGATACGATAGCGATGGGGACAAGAGTGACGACCTGGTGGTGGATGTCTCTAATGAGGACCC
  AGCAACACCCCGGGTGAGCCCAGCACACTCCCCTCCTGAAAATGGGCTGGACAAAGCCCGTGGTCTGAAG
  AAAGATGCCCCCACCAGCCCAGCCTCCGTGGCTTCCTCCAGCAGCACACCTTCCTCCAAGACCAAAGACC
  TTGGTCATAATGACAAATCTTCCACACCTGGGCTCAAGTCCAACACACCAACGCCAAGAAATGATGCCCC
  AACTCCAGGCACCAGCACCACCCCGGGGCTCCGGTCAATGCCGGGCAAACCTCCAGGCATGGACCCGATA
  GGTATAATGGCCTCGGCCCTGCGAACACCCATCACCCTCACCAGCTCCTATCCAGCACCCTTTGCCATGA
  TGAGCCACCACGAGATGAATGGCTCCCTCACCAGCCCAAGCGCCTATGCTGGCCTACACAACATCCCATC
  CCAGATGAGCGCCGCCGCAGCCGCTGCAGCCGCCGCCTATGGCCGATCGCCAATGGTGAGCTTTGGAGCT
  GTTGGTTTTGACCCTCACCCCCCAATGAGGGCCACAGGCCTGCCTTCCAGTCTCGCCTCCATTCCTGGAG
  GGAAACCGGCATACTCCTTCCATGTGAGTGCTGATGGGCAGATGCAACCTGTGCCCTTCCCCCATGATGC
  ACTAGCAGGCCCTGGCATTCCCAGGCATGCCCGGCAGATCAATACGCTCAGCCATGGAGAGGTGGTATGT
  GCTGTGACCATCAGCAACCCCACACGACACGTCTACACAGGCGGCAAGGGCTGTGTGAAGATATGGGACA
  TCAGCCAGCCGGGCAGCAAGAGTCCCATCTCCCAGCTGGACTGCCTGAACAGGGACAACTACATCCGCTC
  GTGCAAGCTTCTCCCCGATGGGCGCACGCTCATTGTGGGTGGTGAGGCCAGCACGCTCACCATCTGGGAC
  CTGGCCTCACCCACACCCCGCATCAAGGCTGAGCTGACGTCCTCGGCTCCAGCCTGTTATGCCCTGGCCA
  TCAGTCCTGATGCCAAAGTCTGTTTTTCCTGCTGCAGCGACGGGAACATTGCGGTTTGGGATCTGCACAA
  CCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGGGGCCAGCTGTATAGACATCTCTCATGATGGC
  ACTAAGCTGTGGACCGGGGGCCTGGACAACACCGTGCGCTCCTGGGACCTACGTGAAGGACGGCAGTTAC
  AGCAACACGATTTCACCTCCCAGATCTTCTCCCTGGGTTACTGCCCCACTGGGGAGTGGCTGGCCGTGGG
  CATGGAGAGCAGCAATGTGGAGGTCCTGCACCACACTAAGCCCGACAAATACCAGCTGCACCTGCACGAG
  AGCTGCGTGCTGTCCCTCAAGTTCGCCTATTGTGGCAAGTGGTTTGTGAGCACTGGGAAAGACAACCTTC
  TCAATGCCTGGAGGACGCCTTATGGAGCCAGCATCTTCCAGTCAAAAGAATCCTCATCTGTCTTGAGCTG
  TGACATTTCAGCGGATGACAAATATATTGTAACAGGCTCTGGTGACAAGAAGGCCACAGTTTACGAGGTC
  ATCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223072 representing NM_009389
  Red=Cloning site Green=Tags(s)
  
  MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYG
  LNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGP
  PVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLAVKDEKNHHELDHRERESSTNNSVSPSESLRA
  SEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARGLK
  KDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPPGMDPI
  GIMASALRTPITLTSSYPAPFAMMSHHEMNGSLTSPSAYAGLHNIPSQMSAAAAAAAAAYGRSPMVSFGA
  VGFDPHPPMRATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQINTLSHGEVVC
  AVTISNPTRHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWD
  LASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDG
  TKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHE
  SCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEV
  IY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009389
• ORF Size: 2316 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009389.2, NP_033415.2
• RefSeq Size: 5172
• RefSeq ORF: 2319
• Locus ID: 21887