Sts (NM_009293) Mouse Tagged ORF Clone

CAT#: MG223101

Sts (GFP-tagged) - Mouse steroid sulfatase (Sts), (10ug). Note: ORF is codon optimized

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sts
• Synonyms: ArsC
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223101 representing NM_009293, codon optimized.
  Due to the complexity of NM_009293, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCGGCCCAGACCCTTGCTGCTGGCGGTGATGGCCGCTACTTTGGCCGATATAATCCTGGCTGCCG
  ATCCCGCGCCCGCCGGACCCGCTCCGAGGCCTCCCAACTTCCTTTTGATCATGGCAGATGATCTTGGAAT
  CGGAGATCTTGGCTGCTACGGAAACAAAACGTTGCGGACTCCACACCTGGACCGCCTGGCTAGGGAGGGA
  GTGAAACTCACTCAACACCTGGCTGCTGCTCCCCTTTGTACTCCTTCCCGGGCCGCCTTCCTGACCGGCC
  GCTACCCACCACGGTCAGGCATGGCTGCCCACGGCCGGGTCGGTGTATACTTGTTTACCGCGTCTAGCGG
  GGGGCTCCCTCCATCCGAAGTCACCATGGCACGCCTGCTGAAGGGACGGGGCTATGCTACCGCTCTTATC
  GGCAAATGGCACCTGGGGCTGTCCTGTAGGGGCGCCACCGACTTTTGTCACCATCCGCTGAGGCATGGCT
  TTGATAGGTTCCTCGGAGTGCCAACTACCAACTTGCGCGACTGCAGACCTGGCGCCGGGACCGTATTTGG
  CCCTGCTCTCCGGGTGTTTGCGGCAGGCCCACTGGCTGCCCTCGGGGCATCACTTGCAGCTATGGCCGCT
  GCCAGATGGGCAGGGCTTGCTAGAGTGCCAGGGTGGGCTTTGGCTGGTACTGCCGCCGCAATGCTGGCCG
  TCGGGGGCCCTCGGTCCGCTAGTTGCTTGGGGTTTAGGCCAGCAAACTGCTTCCTCATGGATGATCTGGC
  TGTTGCACAAAGGCCAACCGACTATGGCGGCCTCACCAGACGGCTGGCTGACGAGGCCGCCCTGTTTCTT
  CGGCGCAATCGCGCAAGACCGTTCCTCCTTTTCCTGAGCTTCCTGCACGTTCACACAGCACACTTCGCCG
  ACCCTGGGTTCGCAGGGCGCTCTCTGCACGGGGCCTACGGGGATTCTGTAGAAGAGATGGACTGGGGTGT
  GGGTAGGGTCCTCGCTGCCCTGGATGAGCTGGGACTGGCTAGAGAAACCCTGGTTTACTTTACTTCAGAC
  CACGGGGCACACGTGGAGGAGCTGGGGCCCAGAGGAGAGCGAATGGGAGGCTCCAACGGTGTCTTCCGAG
  GCGGCAAGGGAAATAACTGGGAAGGCGGCGTCCGCGTGCCTTGCCTGGTCAGATGGCCTCGCGAACTCAG
  TCCTGGACGGGTCGTTGCCGAACCCACCAGCTTGATGGATGTCTTCCCCACAGTAGCCAGACTCGCCGGA
  GCCGAACTGCCAGGCGACAGGGTTATCGATGGTCGCGACCTGATGCCTTTGCTTCGCGGCGATGCGCAGA
  GGAGCGAGCATGAATTCCTTTTTCACTACTGTAACGCCTACTTGCAGGCAGTCCGCTGGCACAATGGAAG
  CGCTGTGTGGAAGGCCTTTTACTTCACCCCTAATTTCGCCCCCGCTGGCGCCAACGGGTGTTTCAGCACT
  CACGTCTGCCTTTGCGCCGGTCCTGCCCACGTTACTGCACACGACCCTCCCCTGCTGTTCGACCTGACTC
  GGGATCCTGGAGAAAGGCGACCTCTTACGCCAGAAGCCGAGCCGCGGCACCGAGAGGTCCTCGATGCAAT
  CGACGCAGCAGCCAGAGCTCACCGAGCACGCCTGCGGCCAGCCCCCGACCAGCTGGCTCCTCGCCACCTT
  ATGTGGAAACCATGGCTGCAGCTGTGGGGTGGAGGGGGTGCTGGGGGTGGCGCCGGTGCACAGGACGATT
  CCGGGCACGCCCACGGCGACGGCAGCCATGCCCATGACGACCCTGGTCACGCCCAAGACCGGGGTGACGA
  TGATGCACATTATGGTGGCCACGCGACCACTCGCACACAGGCCACCCCACGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223101 representing NM_009293
  Red=Cloning site Green=Tags(s)
  
  MPRPRPLLLAVMAATLADIILAADPAPAGPAPRPPNFLLIMADDLGIGDLGCYGNKTLRTPHLDRLAREG
  VKLTQHLAAAPLCTPSRAAFLTGRYPPRSGMAAHGRVGVYLFTASSGGLPPSEVTMARLLKGRGYATALI
  GKWHLGLSCRGATDFCHHPLRHGFDRFLGVPTTNLRDCRPGAGTVFGPALRVFAAGPLAALGASLAAMAA
  ARWAGLARVPGWALAGTAAAMLAVGGPRSASCLGFRPANCFLMDDLAVAQRPTDYGGLTRRLADEAALFL
  RRNRARPFLLFLSFLHVHTAHFADPGFAGRSLHGAYGDSVEEMDWGVGRVLAALDELGLARETLVYFTSD
  HGAHVEELGPRGERMGGSNGVFRGGKGNNWEGGVRVPCLVRWPRELSPGRVVAEPTSLMDVFPTVARLAG
  AELPGDRVIDGRDLMPLLRGDAQRSEHEFLFHYCNAYLQAVRWHNGSAVWKAFYFTPNFAPAGANGCFST
  HVCLCAGPAHVTAHDPPLLFDLTRDPGERRPLTPEAEPRHREVLDAIDAAARAHRARLRPAPDQLAPRHL
  MWKPWLQLWGGGGAGGGAGAQDDSGHAHGDGSHAHDDPGHAQDRGDDDAHYGGHATTRTQATPR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009293
• ORF Size: 1872 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009293.1, NP_033319.1
• RefSeq Size: 2283
• RefSeq ORF: 1875
• Locus ID: 20905
• Gene Summary: Conversion of sulfated steroid precursors to estrogens during pregnancy. [UniProtKB/Swiss-Prot Function]