Abcb4 (NM_008830) Mouse Tagged ORF Clone

CAT#: MG223301

Abcb4 (GFP-tagged) - Mouse ATP-binding cassette sub-family B (MDR/TAP) member 4 (Abcb4), (10ug).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abcb4
• Synonyms: mdr-2; Mdr2; Pgy-2; Pgy2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223301 representing NM_008830, codon optimized.
  Due to the complexity of NM_008830, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTCGAAGCTGCTCGCAACGGGACCGCTCGGAGGCTTGACGGAGATTTTGAGCTGGGGAGCATAA
  GCAACCAGGGCAGGGAAAAGAAAAAAAAGGTGAATTTGATTGGCCTGCTTACCCTTTTCCGCTACTCCGA
  TTGGCAGGACAAACTCTTCATGTTCCTGGGGACTCTGATGGCCATTGCTCATGGATCTGGATTGCCTCTG
  ATGATGATCGTCTTCGGGGAGATGACCGATAAGTTCGTCGATAATACAGGCAACTTCAGCTTGCCAGTCA
  ACTTTAGCCTCTCTATGCTGAACCCCGGACGAATCTTGGAGGAGGAGATGACAAGATATGCTTATTATTA
  TTCAGGACTGGGAGGCGGCGTGCTGGTTGCCGCCTACATACAGGTCAGTTTTTGGACCCTGGCAGCGGGG
  AGACAGATCAAGAAAATCCGACAGAAATTTTTTCATGCAATTCTCAGGCAGGAGATGGGCTGGTTTGATA
  TCAAAGGAACCACTGAGCTTAATACGCGGCTGACCGATGACGTGAGTAAAATCAGCGAGGGCATTGGAGA
  TAAAGTTGGTATGTTTTTCCAGGCCATCGCCACATTTTTTGCAGGATTCATCGTAGGATTTATTCGGGGA
  TGGAAGCTGACTTTGGTTATAATGGCTATCAGCCCTATCCTCGGACTCAGCACAGCTGTGTGGGCCAAGA
  TCCTGAGCACTTTTAGTGATAAAGAGCTCGCAGCATATGCCAAGGCTGGGGCGGTTGCTGAAGAGGCTCT
  GGGTGCCATTAGGACCGTCATCGCATTCGGAGGACAAAATAAAGAATTGGAGCGGTATCAGAAGCATCTC
  GAGAATGCTAAGAAAATTGGCATCAAGAAAGCAATTAGCGCAAATATATCCATGGGGATTGCTTTCCTGC
  TGATATATGCCTCCTACGCCCTGGCCTTTTGGTACGGGTCAACCCTGGTGATCTCTAAGGAGTACACCAT
  TGGCAATGCAATGACGGTTTTTTTTAGTATTCTGATCGGAGCCTTCTCCGTTGGACAGGCCGCCCCTTGC
  ATTGACGCTTTCGCTAACGCCCGGGGAGCAGCCTACGTTATATTTGATATTATTGATAACAATCCTAAAA
  TCGACTCCTTTAGCGAGCGGGGGCACAAGCCGGATAATATTAAAGGGAACCTGGAGTTCAGCGACGTTCA
  CTTCAGTTACCCAAGCCGCGCCAACATCAAAATTCTGAAAGGACTCAACCTCAAAGTTAAAAGCGGACAG
  ACAGTTGCCCTCGTGGGCAACAGTGGTTGCGGCAAGTCAACTACCGTCCAGCTTTTGCAGCGACTTTATG
  ATCCTACAGAAGGGAAAATCAGTATAGATGGGCAGGATATCAGGAATTTTAATGTTCGCTGCCTGAGGGA
  AATCATCGGAGTCGTTTCTCAGGAGCCGGTGCTGTTTTCCACTACCATAGCAGAAAACATTAGGTACGGC
  CGAGGGAATGTGACCATGGATGAGATAGAGAAGGCCGTCAAGGAAGCAAACGCTTACGATTTTATCATGA
  AACTGCCCCAGAAATTTGACACGCTGGTCGGTGACCGCGGGGCTCAGCTGAGTGGAGGCCAAAAGCAGAG
  GATAGCCATCGCTCGGGCCCTGGTGCGCAATCCAAAGATCTTGCTGCTGGACGAGGCCACATCAGCTCTT
  GACACAGAGTCCGAAGCGGAAGTGCAAGCCGCACTCGACAAGGCACGAGAAGGCAGAACTACTATTGTTA
  TCGCTCACAGACTTTCCACCATCAGGAATGCCGATGTTATCGCCGGATTCGAAGACGGGGTGATTGTAGA
  GCAGGGCTCTCACAGCGAGCTCATGAAGAAAGAGGGTATCTATTTTAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223301 representing NM_008830
  Red=Cloning site Green=Tags(s)
  
  MDLEAARNGTARRLDGDFELGSISNQGREKKKKVNLIGLLTLFRYSDWQDKLFMFLGTLMAIAHGSGLPL
  MMIVFGEMTDKFVDNTGNFSLPVNFSLSMLNPGRILEEEMTRYAYYYSGLGGGVLVAAYIQVSFWTLAAG
  RQIKKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDVSKISEGIGDKVGMFFQAIATFFAGFIVGFIRG
  WKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHL
  ENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPC
  IDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDNIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQ
  TVALVGNSGCGKSTTVQLLQRLYDPTEGKISIDGQDIRNFNVRCLREIIGVVSQEPVLFSTTIAENIRYG
  RGNVTMDEIEKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSAL
  DTESEAEVQAALDKAREGRTTIVIAHRLSTIRNADVIAGFEDGVIVEQGSHSELMKKEGIYFR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008830
• ORF Size: 3828 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008830.2, NP_032856.2
• RefSeq Size: 4083
• RefSeq ORF: 3831
• Locus ID: 18670
• MW: 140.4 kDa