Themis (NM_178666) Mouse Tagged ORF Clone

CAT#: MG223329

Themis (GFP-tagged) - Mouse thymocyte selection associated (Themis), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Themis
• Synonyms: E430004N04Rik; Gasp; Spot; thylex; Tsepa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223329 representing NM_178666
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTATCTCTGGAAGAATTTGTCTACTCCCTTGACCTGAGGACCCTCCCCAGGGTCCTAGAAATCC
  AGTCAGGCATCTACTTTGAAGGGTCTGTTTATGAGATGTTTGGCAATGAATGCTGTCTGTCAACAGGAGA
  AGTGATTAAAATCACCGGTCTCAAAATTAAGAAGATGATGGCTGAAATTTGTGAGGGCGCTATTGGAGGT
  TGTGAGTCTCAGAAGCCTTTTGAGCTGCCCATGAATTTTCCAGGTCTCTTTAAGGTCATGGCTGATAAAA
  CTCCATACCTGTCAATTGAAGAAATCACAAGAACCGTTAATATTGGACCCAGCAGACTAGGTCATCCTTG
  CTTTTATCACCTGAAGGATATAAAACTAGAGAATCTCATTATTAAGCAGGGTGAGCCAATCCGGTTCAAC
  TCAGTTGAAGAGATTAATGGAGAAACGCTGGTGAATTGTGGTGTGGTAAGGAATCATCAGAGCCACTCAT
  TTACTTTGCCATTGTCACAAGAAGGAGAATTTTATGAGTGTGAGGATGAACATATCTATACCCTAAAAGA
  AATTGTTGAATGGAAGATTCCCAAGAACAGAACACGGACAGTGAAACTTACAGATTTCTCGAATAAGTGG
  GACTCAACCAATCCATTCCCAGAAGACTTTTATGGTACACTGATTCTCAAACCTGTTTATGAAATCCAAG
  GTGTGCTGAAATTTCAAAAGGACATAGTCCGGATTCTCCCAAGTTTAGACGTTGAAGTCAAAGATATTAC
  TGATTCTTATGATGCTAACTGGTTTCTTCAGTTGCTGTCTACGGACGACCTTTTTGAAATGACCAGCAAA
  GAGTTTCCTGTAGTAGCTGAAGTTGTCGAAATATCTCAAGGGAACCACCTGCCCCAAAGTATTTTGCAGC
  GAGAGAAAACCATTGTCATCCACAAAAAGTACCAGGCTTCGAGGATCTTAGCTTCAGAAATTCGCAGCAA
  TTTCCCTAAAAGACACTTCTTGATCCCTATTAGCTACAAAGGCAAGTTCAAAAGAAGACCACGGGAGTTC
  CCCACGGCCTATGACCTGCAGATAGCTAAGAGCAGGAAAGAAACTCTCCACGTGGTGGCCACCAAAGCTT
  TCCATACACTTCACAAGGAGCTGTCCCCTGTGTCTGTTGGAGACCAGTTTCTAGTGCATCATTCAGAGAC
  CACAGAAGTTGTCTTTGAGGGGACAAGAAAAGTAAACGTTCTGACCTGTGAAAAAGTCCTCAATAAGACC
  CGTGAAGATGCACAGCTTCCTCTATACATGGAAGGAGGTTTTGTAGAGGTGATTCATGATAAGAAACAGT
  ATCAGATTTCCGAGCTCTGTACCCAGTTTTGCTGGCCCTTCAATGTGAAGGTGGCTGTGAGAGATCTCTC
  CATTAAAGATGACATTTTGGCAGCTACCCCAGGACTTCAGTTAGAGGAGGACATCACAGATTCTTATCTA
  CTTATCAGTGACTTTGCAAATCCTGAGGAATGTTGGGAAATTCCCATGAGCCGCTTGAATATGACTGTTC
  GACTAGTTAATGGTAGCAGTTTGCCTGCAGATGCAGGGCTACTTCAAGTCAGGAGTTTTGTAGAAGAGAT
  TACAGAAGAACAGTATTACATGATGAGGAGGTACGAAAGTTCTCTATCACATCCCCCACCTCGCCCTCCC
  AAACATCCCTCAGCTGAGGAAATGAAGCTCACCTTGCTCAGCTTAGCAGAAGAGAGAACAATAAATCTGC
  CCAAGTCTCTCAAGAGTCACCATGTAGACAGACCCAAGAAGCTTCCCTCAGATGAAAGTGGCCAGGATTC
  AAGAGCTCCAGTTGGTTTTCAGAATGATGTGGCTGATGTGGAGAGACAGAAGAGCAAGCATGGGCCACTG
  CAGCCGCAAGCACCACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223329 representing NM_178666
  Red=Cloning site Green=Tags(s)
  
  MALSLEEFVYSLDLRTLPRVLEIQSGIYFEGSVYEMFGNECCLSTGEVIKITGLKIKKMMAEICEGAIGG
  CESQKPFELPMNFPGLFKVMADKTPYLSIEEITRTVNIGPSRLGHPCFYHLKDIKLENLIIKQGEPIRFN
  SVEEINGETLVNCGVVRNHQSHSFTLPLSQEGEFYECEDEHIYTLKEIVEWKIPKNRTRTVKLTDFSNKW
  DSTNPFPEDFYGTLILKPVYEIQGVLKFQKDIVRILPSLDVEVKDITDSYDANWFLQLLSTDDLFEMTSK
  EFPVVAEVVEISQGNHLPQSILQREKTIVIHKKYQASRILASEIRSNFPKRHFLIPISYKGKFKRRPREF
  PTAYDLQIAKSRKETLHVVATKAFHTLHKELSPVSVGDQFLVHHSETTEVVFEGTRKVNVLTCEKVLNKT
  REDAQLPLYMEGGFVEVIHDKKQYQISELCTQFCWPFNVKVAVRDLSIKDDILAATPGLQLEEDITDSYL
  LISDFANPEECWEIPMSRLNMTVRLVNGSSLPADAGLLQVRSFVEEITEEQYYMMRRYESSLSHPPPRPP
  KHPSAEEMKLTLLSLAEERTINLPKSLKSHHVDRPKKLPSDESGQDSRAPVGFQNDVADVERQKSKHGPL
  QPQAPL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178666
• ORF Size: 1908 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_178666.5
• RefSeq Size: 3981
• RefSeq ORF: 1911
• Locus ID: 210757
• Gene Summary: This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]