Brdt (NM_054054) Mouse Tagged ORF Clone

CAT#: MG223393

Brdt (GFP-tagged) - Mouse bromodomain testis-specific (Brdt) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Brdt
• Synonyms: 7420412D09Rik; Brd6; Fsrg3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223393 representing NM_054054
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTGCCAAGTCGACAAACAGCTATTGTTAACCCCCCTCCACCAGAGTACATAAACACTAAGAAGA
  GTGGGCGGTTGACGAATCAGCTGCAGTTCCTACAGAGGGTTGTGCTGAAGGCCCTGTGGAAGCACGGCTT
  CTCTTGGCCTTTCCAACAGCCGGTGGACGCCGTGAAACTAAAGCTGCCTGACTATTACACCATCATAAAA
  ACCCCAATGGATTTAAATACAATTAAGAAGCGGTTGGAAAATAAGTATTATGAGAAGGCTTCCGAATGCA
  TAGAAGACTTCAACACAATGTTCTCAAACTGCTACTTATACAACAAGACTGGAGATGACATTGTTGTTAT
  GGCCCAAGCTCTAGAGAAGTTATTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAAGTTGTGGGT
  GGTAAGGAGAGAATAAAGAAAGATATCCAACAAAAGATAGCGGTTTCCTCTGCTAAGGAACAAATCCCCT
  CCAAAGCGGCAGAGAATGTCTTTAAGCGGCAAGAGATTCCTTCTGGGCTTCCTGACATCTCTCTCTCGCC
  CTTAAACATGGCACAAGAAGCTCCCCCGATCTGTGACTCGCAGTCTCTGGTCCAAATTACAAAGGGTGTG
  AAGAGGCGAGCAGACACAACAACTCCTACCACTTCCATAGCTAAAGCAAGTAGCGAATCTCCTCCGACAC
  TTAGAGAAACAAAGCCAGTGAACATGCCAGTAAAAGAAAATACAGTAAAGAACGTTTTGCCAGACTCTCA
  GCAACAACACAAAGTTTTAAAAACAGTCAAAGTAACGGAGCAATTAAAGCACTGCAGCGAGATTCTTAAA
  GAAATGCTTGCCAAGAAACATTTGCCGTATGCATGGCCCTTCTATAATCCTGTGGATGCTGATGCTTTGG
  GACTCCACAACTACTATGACGTTGTCAAAAATCCCATGGATCTCGGAACGATCAAGGGGAAAATGGACAA
  TCAAGAATATAAGGATGCATATGAATTTGCTGCAGATGTCAGACTGATGTTCATGAACTGTTACAAGTAT
  AATCCTCCCGATCATGAAGTTGTGGCGATGGCTCGGACCCTTCAGGATGTTTTTGAACTACATTTTGCCA
  AGATTCCCGATGAACCTATTGAGAGTATGCATGCATGCCACCTTACAACAAACAGTGCACAAGCCCTCAG
  CAGAGAAAGCAGCAGCGAGGCCTCCTCGGGGGACGCCTCTTCTGAAGACTCTGAGGATGAACGAGTGCAG
  CATCTTGCCAAGCTTCAGGAGCAGCTTAATGCTGTTCATCAGCAGCTGCAAGTTCTGTCCCAAGTTCCAT
  TACGTAAGCTGAAGAAAAAGAATGAGAAGTCCAAAAGGGCGCCAAAAAGGAAAAAGGTTAATAACAGAGA
  TGAAAACCCAAGGAAAAAGCCTAAGCAAATGAAGCAAAAGGAGAAGGCCAAAATCAATCAACCAAAGAAG
  AAGAAGCCGCTTTTGAAATCGGAAGAGGAAGACAATGCGAAGCCTATGAACTACGATGAGAAAAGGCAGC
  TGAGCTTGGACATAAACAAACTGCCTGGAGACAAGCTTGGTCGAATAGTTCACATAATACAGTCAAGGGA
  GCCTTCCCTGAGAAACTCCAACCCGGACGAGATCGAGATCGACTTTGAGACCCTGAAGGCATCCACACTT
  AGAGAACTGGAGAAGTATGTCCTGGCCTGTCTGAGGAAGAGGTCGCTGAAGCCCCAAGCAAAGAAGGTTG
  TCAGGTCTAAGGAGGAGCTCCATTCTGAAAAAAAGCTGGAGCTGGAGAGGCGGTTGCTGGACGTCAACAA
  TCAGCTGAATTGTAGGAAACGGCAAACGAAACGTCCAGCAAAAGTGGAGAAGCCGCCCCCGCCCCCGCCC
  CCGCCCCCGCCCCCGCCGCCTCCCCCGGAGCTGGCCAGTGGGTCTCGCCTGACCGACAGCAGCAGCAGCA
  GCGGCAGCGGCAGCGGCAGCAGCAGCAGCAGCAGCGGCAGCAGCAGCAGCAGCAGCTCCTCGGGGTCCGC
  GAGCAGCAGCAGCGACTCGAGCTCCTCCGACAGCAGCGATTCTGAACCAGAAATATTTCCTAAATTTACT
  GGAGTAAAACAGAATGATTTACCTCCAAAAGAAAATATAAAGCAGATACAATCTTCTGTGCAAGACATAA
  CCTCTGCTGAAGCTCCCCTTGCTCAGCAGAGCACAGCACCATGCGGAGCCCCAGGGAAGCACTCCCAGCA
  GATGCTTGGTTGCCAAGTGACACAGCATCTGCAGGCCACAGAGAACACTGCATCTGTGCAGACTCAGCCT
  CTTTCAGGTGATTGCAAGCGAGTATTGCTTGGCCCCCCTGTGGTGCACACCTCTGCTGAGAGCCTCACAG
  TGCTGGAGCCAGAATGCCATGCTCCCGCGCAGAAGGATATAAAGATTAAGAATGCAGACTCTTGGAAAAG
  TTTAGGGAAGCCAGTGAAAGCATCAAGTGTACTGAAGTCTTCAGATGAGCTTTTCAACCAGTTTAGAAAA
  GCTGCAATAGAAAAGGAGGTAAAGGCCCGGACACAGGAGCAGATGCGGAAACACCTGGAGCATAATGCGA
  AGGACCCAAAAGTATCTCAAGAAAATCAGAGGGAGCCTGGAAGTGGATTGACTCTAGAATCTTTATCAAG
  TAAAGTGCAAGATAAGTCTCTTGAAGAAGATCAGAGTGAACAGCAGCCACCGTCGGAAGCTCAAGACGTA
  AGCAAGCTCTGGCTTCTCAAAGACCGGAATCTAGCGAGGGAGAAAGAGCAAGAGCGCAGGAGAAGAGAAG
  CAATGGCGGGTACCATTGATATGACTCTTCAAAGTGACATTATGACAATGTTTGAAAATAACTTTGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223393 representing NM_054054
  Red=Cloning site Green=Tags(s)
  
  MSLPSRQTAIVNPPPPEYINTKKSGRLTNQLQFLQRVVLKALWKHGFSWPFQQPVDAVKLKLPDYYTIIK
  TPMDLNTIKKRLENKYYEKASECIEDFNTMFSNCYLYNKTGDDIVVMAQALEKLFMQKLSQMPQEEQVVG
  GKERIKKDIQQKIAVSSAKEQIPSKAAENVFKRQEIPSGLPDISLSPLNMAQEAPPICDSQSLVQITKGV
  KRRADTTTPTTSIAKASSESPPTLRETKPVNMPVKENTVKNVLPDSQQQHKVLKTVKVTEQLKHCSEILK
  EMLAKKHLPYAWPFYNPVDADALGLHNYYDVVKNPMDLGTIKGKMDNQEYKDAYEFAADVRLMFMNCYKY
  NPPDHEVVAMARTLQDVFELHFAKIPDEPIESMHACHLTTNSAQALSRESSSEASSGDASSEDSEDERVQ
  HLAKLQEQLNAVHQQLQVLSQVPLRKLKKKNEKSKRAPKRKKVNNRDENPRKKPKQMKQKEKAKINQPKK
  KKPLLKSEEEDNAKPMNYDEKRQLSLDINKLPGDKLGRIVHIIQSREPSLRNSNPDEIEIDFETLKASTL
  RELEKYVLACLRKRSLKPQAKKVVRSKEELHSEKKLELERRLLDVNNQLNCRKRQTKRPAKVEKPPPPPP
  PPPPPPPPPELASGSRLTDSSSSSGSGSGSSSSSSGSSSSSSSSGSASSSSDSSSSDSSDSEPEIFPKFT
  GVKQNDLPPKENIKQIQSSVQDITSAEAPLAQQSTAPCGAPGKHSQQMLGCQVTQHLQATENTASVQTQP
  LSGDCKRVLLGPPVVHTSAESLTVLEPECHAPAQKDIKIKNADSWKSLGKPVKASSVLKSSDELFNQFRK
  AAIEKEVKARTQEQMRKHLEHNAKDPKVSQENQREPGSGLTLESLSSKVQDKSLEEDQSEQQPPSEAQDV
  SKLWLLKDRNLAREKEQERRRREAMAGTIDMTLQSDIMTMFENNFD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_054054
• ORF Size: 2868 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_054054.2, NP_473395.2
• RefSeq Size: 4710
• RefSeq ORF: 2871
• Locus ID: 114642
• Gene Summary: The protein encoded by this gene belongs to the BET protein family. BET proteins have two N-terminal bromodomains and one C-terminal extraterminal domain (ET domain). BET proteins regulate chromatin reorganization via binding to acetylated histones. This gene is thought to play a role in the transcriptional regulation of spermatogenesis. Although referred to as testis-specific bromodomain (Brdt) protein, RT-PCR indicates that this gene is expressed in both mouse oocytes and testes. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Jul 2008]