Pde4b (NM_001177981) Mouse Tagged ORF Clone

CAT#: MG223615

Pde4b (GFP-tagged) - Mouse phosphodiesterase 4B cAMP specific (Pde4b) transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pde4b
• Synonyms: Dpde4; dunce; R74983
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223615 representing NM_001177981
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAGGCAAACTATTTATTATCTGTTTCTTGGGGTTATATCAAGTTCAAAAGGATGCTGAACCGGG
  AGCTGACACACCTCTCAGAGATGAGCAGATCAGGGAACCAGGTGTCTGAGTACATTTCAAACACGTTCTT
  AGACAAGCAGAACGATGTGGAAATCCCATCTCCCACGCAGAAGGACAGGGAGAAGAAGAAGAAGCAGCAG
  CTCATGACCCAGATAAGTGGAGTGAAGAAACTGATGCACAGCTCAAGCCTGAACAACACAAGCATCTCAC
  GCTTCGGAGTCAACACGGAAAATGAGGATCATCTAGCCAAGGAGCTGGAAGACCTGAACAAATGGGGCCT
  TAACATCTTCAATGTGGCTGGGTACTCACATAATCGGCCCCTTACGTGCATCATGTATGCAATATTCCAG
  GAAAGAGACCTTCTGAAGACGTTTAAAATCTCATCTGACACCTTTGTAACCTACATGATGACTTTAGAAG
  ACCATTACCATTCTGATGTGGCATATCACAACAGCCTGCATGCTGCTGACGTGGCCCAGTCAACTCACGT
  TCTCCTTTCTACGCCGGCACTGGATGCTGTCTTCACAGACCTGGAAATCCTGGCTGCCATTTTTGCAGCT
  GCCATCCATGATGTCGATCATCCTGGAGTCTCCAATCAGTTTCTCATCAATACAAATTCTGAACTTGCTT
  TGATGTATAATGATGAATCTGTTCTGGAAAACCATCACCTTGCTGTGGGATTCAAATTGCTACAAGAGGA
  ACACTGCGACATCTTTCAGAATCTTACCAAGAAGCAACGCCAGACACTCAGGAAAATGGTGATTGACATG
  GTGTTGGCAACTGATATGTCCAAACACATGAGCCTCCTGGCAGACCTTAAAACAATGGTAGAAACCAAGA
  AGGTGACAAGCTCCGGTGTTCTCCTCCTGGACAACTATACTGACCGGATACAGGTTCTTCGCAACATGGT
  ACACTGTGCAGACCTGAGCAACCCCACCAAGTCCTTGGAATTGTATCGGCAATGGACCGATCGTATCATG
  GAGGAGTTTTTCCAGCAGGGAGACAAAGAACGGGAGAGGGGAATGGAGATTAGCCCAATGTGTGATAAGC
  ACACAGCTTCTGTGGAAAAATCCCAGGTTGGTTTCATTGACTACATTGTCCATCCACTGTGGGAGACCTG
  GGCAGACCTGGTTCAACCGGATGCTCAAGATATTCTGGATACACTAGAAGATAACAGGAACTGGTACCAG
  AGTATGATACCCCAGAGCCCTTCCCCGCCACTGGATGAGAGGAGCAGGGACTGCCAAGGCCTGATGGAGA
  AGTTTCAGTTTGAACTGACCCTTGAGGAAGAGGATTCTGAGGGACCGGAAAAGGAGGGAGAAGGCCACAG
  CTATTTCAGCAGCACAAAGACGCTTTGTGTGATTGATCCAGAGAACAGGGATTCTCTGGAAGAGACTGAC
  ATAGACATTGCAACAGAAGACAAGTCTCCGATCGACACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223615 representing NM_001177981
  Red=Cloning site Green=Tags(s)
  
  MPEANYLLSVSWGYIKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQNDVEIPSPTQKDREKKKKQQ
  LMTQISGVKKLMHSSSLNNTSISRFGVNTENEDHLAKELEDLNKWGLNIFNVAGYSHNRPLTCIMYAIFQ
  ERDLLKTFKISSDTFVTYMMTLEDHYHSDVAYHNSLHAADVAQSTHVLLSTPALDAVFTDLEILAAIFAA
  AIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQTLRKMVIDM
  VLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIM
  EEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQ
  SMIPQSPSPPLDERSRDCQGLMEKFQFELTLEEEDSEGPEKEGEGHSYFSSTKTLCVIDPENRDSLEETD
  IDIATEDKSPIDT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177981
• ORF Size: 1509 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001177981.1, NP_001171452.1
• RefSeq Size: 3746
• RefSeq ORF: 1512
• Locus ID: 18578