Unc5a (NM_153131) Mouse Tagged ORF Clone

CAT#: MG223732

  • TrueORF®

Unc5a (GFP-tagged) - Mouse unc-5 homolog A (C. elegans) (Unc5a), (10ug)


  "NM_153131" in other vectors (4)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Unc5a
Synonyms mKIAA1976; Unc5h1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG223732 representing NM_153131
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGTCCGGCCCGGCCTGTGGCCAGCGCTCCTGGGCATAGTCCTCACTGCCTGGCTTCGTGGTTCGG
GTGCCCAGCAGAGTGCCACAGTGGCCAACCCAGTGCCTGGTGCCAACCCGGACCTGCTGCCCCACTTCCT
GGTAGAGCCGGAGGACGTGTACATTGTCAAGAACAAGCCCGTGCTGCTGGTGTGCAAGGCTGTGCCCGCC
ACCCAGATCTTCTTCAAGTGCAACGGGGAATGGGTTCGCCAGGTCGATCACGTCATTGAACGCAGCACTG
ACGGCAGCAGCGGATTGCCAACCATGGAGGTCCGGATCAACGTATCAAGGCAGCAGGTCGAGAAAGTGTT
TGGGCTGGAGGAGTACTGGTGCCAGTGTGTGGCATGGAGCTCCTCAGGAACCACCAAAAGCCAGAAGGCC
TACATCCGGATTGCCTATTTGCGCAAGAACTTTGAGCAGGAGCCGCTGGCCAAGGAAGTGTCACTGGAGC
AAGGCATTGTGCTACCTTGTCGCCCCCCGGAAGGAATCCCCCCAGCTGAGGTGGAGTGGCTCCGAAATGA
GGACCTCGTGGACCCCTCCCTCGACCCCAATGTGTACATCACACGGGAGCACAGCCTAGTCGTGCGGCAG
GCCCGCCTGGCCGACACTGCCAACTACACCTGCGTGGCCAAGAACATCGTGGCCCGTCGCCGAAGCGCCT
CTGCGGCCGTCATTGTTTATGTGAACGGTGGGTGGTCGACGTGGACCGAGTGGTCCGTCTGCAGTGCCAG
CTGTGGGCGTGGCTGGCAGAAACGGAGCCGGAGCTGCACCAACCCGGCACCTCTCAACGGGGGCGCCTTC
TGTGAGGGGCAGAATGTCCAGAAAACAGCCTGCGCCACTCTGTGCCCAGTGGATGGGAGCTGGAGCCCAT
GGAGTAAGTGGTCAGCCTGCGGGCTTGACTGCACCCACTGGCGGAGCCGGGAGTGCTCCGACCCAGCGCC
CCGCAACGGAGGTGAGGAGTGCCGGGGTGCTGACCTGGACACCCGCAACTGTACCAGTGACCTCTGCCTG
CACACCTCTTCCGGCCCCGAGGACGTGGCTCTCTACATCGGCCTCGTCGCCGTGGCCGTGTGCCTCATCT
TGCTGCTGCTGGTCCTCGTCCTCATCTACTGCCGCAAGAAGGAAGGACTGGACTCAGACGTGGCTGACTC
ATCCATCCTTACCTCAGGCTTCCAGCCTGTCAGCATCAAGCCCAGCAAAGCAGACAATCCCCATCTGCTC
ACCATCCAACCGGACCTCAGCACCACCACGACCACCTACCAGGGCAGCCTGTGTCCCCGGCAGGATGGAC
CCAGCCCCAAGTTCCAGCTCTCTAATGGTCACCTGCTCAGCCCACTGGGCAGTGGCCGCCATACGCTGCA
CCACAGCTCCCCCACCTCTGAGGCTGAGGACTTCGTCTCCCGCCTCTCCACCCAAAACTACTTTCGTTCT
CTGCCCCGCGGTACCAGCAACATGGCCTATGGGACCTTCAACTTCCTCGGGGGCCGGCTGATGATCCCTA
ACACAGGAATCAGCCTCCTCATACCCCCGGACGCCATCCCCCGAGGAAAGATCTACGAGATCTACCTCAC
TCTGCACAAGCCAGAAGACGTGAGGTTGCCCCTAGCTGGCTGTCAGACCCTGCTGAGTCCTATCGTTAGC
TGTGGGCCCCCAGGAGTCCTGCTCACCCGGCCAGTCATCCTTGCCATGGACCACTGCGGGGAGCCCAGTC
CCGACAGCTGGAGCCTGCGCCTCAAAAAGCAGTCCTGTGAGGGCAGCTGGGAGGACGTGCTGCACCTTGG
TGAGGAGTCGCCCTCTCATCTCTACTACTGCCAGCTGGAGGCCGGGGCCTGCTATGTCTTCACCGAGCAG
CTAGGCCGCTTTGCCCTGGTGGGAGAGGCCCTCAGCGTGGCTGCCACCAAGCGCCTCAGGCTCCTTCTGT
TTGCCCCTGTGGCCTGTACGTCCCTCGAGTACAACATCCGAGTGTACTGCCTGCACGACACCCACGATGC
TCTCAAGGAGGTGGTGCAGCTGGAGAAGCAGCTGGGTGGACAGCTGATCCAGGAGCCCCGTGTCCTGCAC
TTCAAAGACAGTTACCACAACCTACGTCTGTCCATCCACGACGTGCCCAGCTCCCTGTGGAAGAGCAAGC
TCCTTGTCAGCTACCAGGAGATCCCTTTTTACCACATCTGGAATGGCACTCAGCAGTATCTGCACTGCAC
CTTCACCCTGGAGCGCGTCAATGCCAGCACCAGCGACCTGGCCTGCAAGGTGTGGGTGTGGCAGGTGGAG
GGAGATGGACAGAGCTTCAACATCAACTTTAACATCACTAAGGACACGAGGTTTGCTGAAATGCTGGCTC
TGGAGAGTGAAGGGGGGGTCCCAGCCCTGGTGGGCCCCAGTGCCTTCAAGATCCCCTTCCTCATTCGGCA
AAAGATCATTACCAGCCTGGACCCACCCTGCAGCCGGGGCGCCGACTGGCGAACTCTAGCCCAGAAACTT
CACCTGGACAGCCATCTTAGCTTCTTTGCCTCCAAGCCCAGCCCTACAGCCATGATCCTCAACCTATGGG
AGGCGCGGCACTTCCCCAACGGCAACCTCGGCCAGCTGGCCGCAGCTGTGGCCGGACTGGGCCAGCCAGA
TGCTGGCCTCTTCACCGTGTCAGAGGCCGAGTGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG223732 representing NM_153131
Red=Cloning site Green=Tags(s)

MAVRPGLWPALLGIVLTAWLRGSGAQQSATVANPVPGANPDLLPHFLVEPEDVYIVKNKPVLLVCKAVPA
TQIFFKCNGEWVRQVDHVIERSTDGSSGLPTMEVRINVSRQQVEKVFGLEEYWCQCVAWSSSGTTKSQKA
YIRIAYLRKNFEQEPLAKEVSLEQGIVLPCRPPEGIPPAEVEWLRNEDLVDPSLDPNVYITREHSLVVRQ
ARLADTANYTCVAKNIVARRRSASAAVIVYVNGGWSTWTEWSVCSASCGRGWQKRSRSCTNPAPLNGGAF
CEGQNVQKTACATLCPVDGSWSPWSKWSACGLDCTHWRSRECSDPAPRNGGEECRGADLDTRNCTSDLCL
HTSSGPEDVALYIGLVAVAVCLILLLLVLVLIYCRKKEGLDSDVADSSILTSGFQPVSIKPSKADNPHLL
TIQPDLSTTTTTYQGSLCPRQDGPSPKFQLSNGHLLSPLGSGRHTLHHSSPTSEAEDFVSRLSTQNYFRS
LPRGTSNMAYGTFNFLGGRLMIPNTGISLLIPPDAIPRGKIYEIYLTLHKPEDVRLPLAGCQTLLSPIVS
CGPPGVLLTRPVILAMDHCGEPSPDSWSLRLKKQSCEGSWEDVLHLGEESPSHLYYCQLEAGACYVFTEQ
LGRFALVGEALSVAATKRLRLLLFAPVACTSLEYNIRVYCLHDTHDALKEVVQLEKQLGGQLIQEPRVLH
FKDSYHNLRLSIHDVPSSLWKSKLLVSYQEIPFYHIWNGTQQYLHCTFTLERVNASTSDLACKVWVWQVE
GDGQSFNINFNITKDTRFAEMLALESEGGVPALVGPSAFKIPFLIRQKIITSLDPPCSRGADWRTLAQKL
HLDSHLSFFASKPSPTAMILNLWEARHFPNGNLGQLAAAVAGLGQPDAGLFTVSEAEC

TRTRRLE - GFP Tag - V
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_153131
ORF Size 2694 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Reference Data
RefSeq NM_153131.3, NP_694771.1
RefSeq Size 3974
RefSeq ORF 2697
Locus ID 107448

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