Sema5a (NM_009154) Mouse Tagged ORF Clone

CAT#: MG223912

Sema5a (GFP-tagged) - Mouse sema domain seven thrombospondin repeats (type 1 and type 1-like) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 5A (Sema5a), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sema5a
• Synonyms: 5930434A13; 9130201M22Rik; AI464145; Semaf; semF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223912 representing NM_009154
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGGAGCCTGCATCCTTGCATGGCTGTTCTCAAGCCTGGGAGTGTGGAGACTTGCTAGGCCCGAGA
  CCCAGGACCCTGCCAAGTGCCAGAGAGCTGAGCACCCTGTCGTCTCTTACAAAGAAATTGGCCCCTGGTT
  ACGGGAGTTCAGAGCCGAGAATGCTGTGGATTTCTCGAGGTTAACATTTGACCCAGGACAGAAAGAACTT
  GTCGTAGGAGCGAGAAACTATCTCTTCAGATTAGAGCTTGAGGATCTGTCTCTCATCCAGGCTGTGGAGT
  GGGAGTGTGACGAAGCCACCAAGAAGGCCTGTTACAGCAAGGGCAAATCGAAGGAGGAATGTCAGAACTA
  CATACGAGTGCTCTTAGTTGGTGGGGACCGGCTATTCACCTGTGGGACCAATGCCTTCACACCTGTCTGT
  ACCATCCGCTCGTTAAGTAACCTGACTGAGATCCATGATCAGATCAGTGGCATGGCCCGCTGTCCCTACA
  GTCCCCAGCACAATTCTACCGCCCTACTCACTGCCAGTGGTGAGCTCTATGCTGCAACAGCCATGGATTT
  CCCAGGGCGAGATCCAGCCATTTACCGAAGTCTGGGCACTTTGCCTCCTCTTCGAACTGCACAGTACAAC
  TCCAAATGGCTCAATGAACCAAACTTTGTGTCTTCCTACGACATCGGAAATTTCACCTACTTCTTCTTCC
  GAGAAAACGCCGTGGAACATGACTGTGGGAAGACGGTGTTCTCCAGGGCTGCCCGGGTCTGTAAAAATGA
  CATTGGAGGGCGATTTCTTCTGGAGGACACCTGGACTACCTTCATGAAGGCTCGCCTCAACTGCTCCCGG
  CCTGGTGAGGTGCCCTTCTACTACAATGAGCTGCAGGGTACTTTCTTCCTGCCGGAGCTGGATCTGATCT
  ATGGTATCTTCACCACCAATGTAAACAGCATCGCTGCCTCTGCTGTCTGTGTCTTCAACCTGAGCGCCAT
  CTCACAGGCCTTCAATGGACCCTTCAAGTACCAAGAAAACTCTCGCTCAGCCTGGCTGCCTTATCCCAAC
  CCCAACCCCAATTTTCAGTGCGGCACCATGGACCAGGGCCTGTACGTAAACCTGACGGAAAGAAACCTAC
  AGGATGCTCAGAAGTTCATTCTGATGCATGAAGTGGTGCAGCCAGTGACCACAGTGCCCTCTTTCATGGA
  GGACAACAGCCGCTTCTCCCACTTAGCTGTTGATGTTGTGCAAGGCAGGGAGACTCTGGTCCACATCATT
  TATCTGGCCACAGATTATGGCACCATTAAGAAAGTACGAGCTCCCCTGAGTCAGAGCTCAGGCAGCTGTT
  TGCTGGAAGAGATTGAGCTTTTCCCAGAGAGGAGGAGTGAGCCCATCAGGAGCCTGCAGATCCTCCACAG
  CCAGAGTGTCCTATTTGTGGGACTACAGGAGCATGTGGCCAAGATCCCCCTGAAGAGGTGCCACTTCCAT
  CAAACACGCAGTGCCTGCATTGGCGCTCAGGACCCTTACTGTGGCTGGGATGCGGTGATGAAGAAATGCA
  CCAGCCTGGAGGAGAGTCTGAGCATGACCCAGTGGGATCAGAGCATCCCCACCTGTCCGACCAGAAATCT
  CACTGTAGATGGGAGCTTTGGCCCATGGTCACCGTGGACACCCTGTACACACACTGATGGCACTGCTGTG
  GGCTCCTGCCTCTGCCGGTCCCGCTCCTGTGACAGCCCAGCTCCTCAGTGTGGTGGTTGGCAGTGTGAGG
  GCCCTAGAATGGAGATCACCAACTGTTCCAGGAATGGAGGCTGGACTCCCTGGACCTCCTGGTCTCCGTG
  CAGCACGACCTGTGGCATTGGCTTCCAAGTGCGGCAGCGATCCTGCAGCAACCCCACGCCCAGGCATGGC
  GGGCGCGTGTGCGTGGGCCAGAACAGGGAGGAAAGATACTGCAATGAACATTTGCTCTGCCCACCACACG
  TGTTCTGGACAGGCTGGGGACCTTGGGAACGGTGCACAGCCCAGTGCGGGGGTGGCATTCAAGCCCGCCG
  GAGGACCTGTGAAAATGGGCCTGACTGTGCAGGATGCAATGTGGAATACCAGCCTTGTAACACCAATGCA
  TGCCCGGAGCTGAAGAAGACCACACCCTGGACACCCTGGACCCCTGTCAACATCTCTGACAATGGAGGTC
  ACTATGAGCAGCGTTTCCGCTATACCTGTAAAGCTCGCCTGCCAGATCCAAATTTGCTGGAAGTAGGAAG
  ACAGAGGATAGAAATGCGGTACTGTTCCAGCGATGGAACCAGTGGCTGCTCCACAGACGGACTTTCTGGA
  GACTTTCTAAGAGCTGGGAGATACTCTGCTCATACAGTCAATGGGGCATGGTCAGCCTGGACTTCCTGGT
  CACAGTGCAGCAGAGACTGCAGCAGGGGCATTCGGAACCGGAAGCGTGTTTGCAACAACCCAGAACCCAA
  GTTTGGGGGCATGCCATGCCTTGGCCCATCGCTGGAGTTCCAGGAATGCAACATTTTACCTTGTCCAGTG
  GATGGTGTGTGGTCTTGCTGGTCATCCTGGTCTAAATGTTCAGCAACCTGTGGAGGTGGCCACTACATGA
  GAACCCGTTCTTGTTCGAATCCAGCCCCAGCATATGGAGGGGACATCTGCCTGGGACTGCACACAGAAGA
  GGCACTCTGCAACACACAGACCTGCCCAGAAAGCTGGTCAGAGTGGTCAGACTGGTCTGTGTGCGATGCA
  TCTGGTACCCAGGTCCGTGCTCGGCAGTGCATCCTTCTGTTTCCAGTGGGCAGCCAATGTTCTGGAAATA
  CTACAGAGAGCCGGCCTTGTGTATTTGACTCTAATTTCATCCCAGAAGTATCTGTGGCAAGATCCAGCAG
  CGTAGAAGAAAAAAGGTGTGGAGAGTTCAACATGTTCCACATGATGGCCGTGGGGCTTAGCAGTTCCATT
  CTTGGCTGCCTCCTCACACTGCTTGTCTACACCTACTGCCAGAGGTACCAGCAGCAGTCCCATGATGCAA
  CTGTCATCCACCCTGTCTCTCCTGCCGCCCTCAACAGCAGCATAACTAACCACATCAACAAACTGGACAA
  ATATGATTCTGTGGAGGCCATCAAGGCATTTAACAAAAACAACTTGATCCTAGAGGAGAGAAACAAATAC
  TTCAACCCACATCTCACTGGGAAGACCTATTCCAACGCCTACTTTACAGATCTCAACAATTATGATGAAT
  AC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223912 representing NM_009154
  Red=Cloning site Green=Tags(s)
  
  MKGACILAWLFSSLGVWRLARPETQDPAKCQRAEHPVVSYKEIGPWLREFRAENAVDFSRLTFDPGQKEL
  VVGARNYLFRLELEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVC
  TIRSLSNLTEIHDQISGMARCPYSPQHNSTALLTASGELYAATAMDFPGRDPAIYRSLGTLPPLRTAQYN
  SKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSR
  PGEVPFYYNELQGTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAISQAFNGPFKYQENSRSAWLPYPN
  PNPNFQCGTMDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHLAVDVVQGRETLVHII
  YLATDYGTIKKVRAPLSQSSGSCLLEEIELFPERRSEPIRSLQILHSQSVLFVGLQEHVAKIPLKRCHFH
  QTRSACIGAQDPYCGWDAVMKKCTSLEESLSMTQWDQSIPTCPTRNLTVDGSFGPWSPWTPCTHTDGTAV
  GSCLCRSRSCDSPAPQCGGWQCEGPRMEITNCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHG
  GRVCVGQNREERYCNEHLLCPPHVFWTGWGPWERCTAQCGGGIQARRRTCENGPDCAGCNVEYQPCNTNA
  CPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLPDPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSG
  DFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKFGGMPCLGPSLEFQECNILPCPV
  DGVWSCWSSWSKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQTCPESWSEWSDWSVCDA
  SGTQVRARQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMMAVGLSSSI
  LGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSITNHINKLDKYDSVEAIKAFNKNNLILEERNKY
  FNPHLTGKTYSNAYFTDLNNYDEY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009154
• ORF Size: 3222 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009154.2, NP_033180.2
• RefSeq Size: 10809
• RefSeq ORF: 3225
• Locus ID: 20356