Kcnk9 (NM_001033876) Mouse Tagged ORF Clone

CAT#: MG223955

Kcnk9 (GFP-tagged) - Mouse potassium channel subfamily K member 9 (Kcnk9), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kcnk9
• Synonyms: Task3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223955 representing NM_001033876
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGGCAGAACGTGCGTACCCTGTCCTTGATCGCCTGTACCTTCACCTACCTGCTGGTCGGTGCCG
  CGGTGTTCGACGCCCTCGAGTCGGACCATGAGATGCGCGAGGAGGAGAAACTTAAAGCCGAAGAAGTCCG
  TCTCAGAGGCAAGTACAACATCAGCTCCGATGACTACCAGCAGCTGGAGCTGGTAATCCTGCAGTCTGAG
  CCCCACCGCGCTGGTGTCCAGTGGAAATTCGCCGGTTCCTTCTACTTCGCCATCACTGTCATCACAACTA
  TCGGATATGGACATGCTGCACCTGGAACTGATGCTGGCAAGGCCTTCTGTATGTTCTACGCTGTGCTGGG
  TATCCCCCTGACGCTGGTTATGTTCCAGAGCCTGGGCGAGCGCATGAACACCTTCGTGCGCTACCTGCTG
  AAACGTATCAAGAAGTGCTGTGGCATGCGCAACACTGAAGTTTCTATGGAGAACATGGTGACCGTCGGCT
  TCTTTTCCTGCATGGGCACCCTGTGCCTTGGGGCGGCTGCCTTTTCCCAGTGTGAAGATTGGAGCTTCTT
  CCATGCCTACTACTACTGCTTCATAACATTGACTACTATAGGGTTCGGCGATTTTGTGGCCCTGCAGGCC
  AAGGGTGCGCTGCAGAGGAAGCCATTCTATGTGGCCTTCAGCTTCATGTATATCCTGGTGGGCCTGACCG
  TCATCGGTGCCTTCCTCAATCTTGTGGTCCTGCGATTCCTGACCATGAATACCGATGAGGAGCTACTGGA
  GGGAGAAGTTGCGGAGATTCTTGCTGGAAATCCAAGAAGAGTGTCTGTCCGTGCGCCTCAGAGACGCAAG
  AGGCACCACGCCATGTACTTCCTCAGGAAATACGGCAGAACCCTGTGCTATCTCTGCTTCCCTGGTACCA
  ACTGGGGTAAAGATGATGACGATGATGATGATGATGATGTCGTCGATAATGTTGTTGTTACTGCTCCTAT
  TTCTGCTCCTGCGCCTGCTCCTGCTCCTGCGCCTGCTCCTGCTGCTGTGGCTGCTGGTGCCACTATCAGG
  AGTGTCCGGGCTACCGTCCACACCGTTTCCTGCAGGGTTGAAGAGATCCCTCCAGACGTGCTGAGGAACA
  CCTACTTCCGGTCCGTTTTCGGCGCCATTCCTCCTGGAATGCACACCTGCGGGGACCATAGGCTGCACCT
  CCGTCGCAAGTCCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223955 representing NM_001033876
  Red=Cloning site Green=Tags(s)
  
  MKRQNVRTLSLIACTFTYLLVGAAVFDALESDHEMREEEKLKAEEVRLRGKYNISSDDYQQLELVILQSE
  PHRAGVQWKFAGSFYFAITVITTIGYGHAAPGTDAGKAFCMFYAVLGIPLTLVMFQSLGERMNTFVRYLL
  KRIKKCCGMRNTEVSMENMVTVGFFSCMGTLCLGAAAFSQCEDWSFFHAYYYCFITLTTIGFGDFVALQA
  KGALQRKPFYVAFSFMYILVGLTVIGAFLNLVVLRFLTMNTDEELLEGEVAEILAGNPRRVSVRAPQRRK
  RHHAMYFLRKYGRTLCYLCFPGTNWGKDDDDDDDDDVVDNVVVTAPISAPAPAPAPAPAPAAVAAGATIR
  SVRATVHTVSCRVEEIPPDVLRNTYFRSVFGAIPPGMHTCGDHRLHLRRKSI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033876
• ORF Size: 1206 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033876.1, NP_001029048.1
• RefSeq Size: 1209 bp
• RefSeq ORF: 1209 bp
• Locus ID: 223604
• Cytogenetics: 15 D3