Alox12b (NM_009659) Mouse Tagged ORF Clone

CAT#: MG223970

Alox12b (GFP-tagged) - Mouse arachidonate 12-lipoxygenase 12R type (Alox12b), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Alox12b
• Synonyms: 12R-LOX; Aloxe2; e-LOX2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223970 representing NM_009659
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCTATAAAGTCAAGGTGGCCACGGGCACTGACTTCTTTTCGGGGACGCTGGATTCAATTTCAC
  TGACCATCGTGGGGACCCAAGGAGAGAGCCATAAGCAGCGGTTGAACCACTTTGGGAGAGACTTTGCCAC
  GGGGGCGGTGGATGACTACACGGTACAGTGCCAGCAGGACCTGGGGGAGCTTATCATCATCCGTCTGCAC
  AAGGAGCCTCATTCCTTCCTAGCCAAGGACCCCTGGTACTGCAACTATGTGCAGATCTGTGCTCCCGACT
  GCCGTGTCTACCACTTCCCAGCTTACCAGTGGATGGATGGCTATGAGACCCTGGCACTCCGGGAGGCCAC
  AGGAAAGATAACAGCAGACGACACACTCCCTATCCTTTTGGAACACAGACAAGAAGAGATCAGAGCCAAG
  AAGGACTTCTACCACTGGAGGGTCTTTGTTCCCGGCCTTCCCAACTATGTGGATATCCCCAGTTACCATC
  CTCCTCCCCGACGGTGCCGCAATCCCAACCGGCCTGAGTGGGATGGTTATATTCCCGGATTCCCAATTCT
  CATCAACATTAAGGCTACCAGGTTCTTAAACTCAAATCTTCGTTTCTCCTTCGTCAAGACAGCCTCCTTC
  TTCTACCGCCTAGGGCCCATGGCGCTGGCCTTCAAACTGCGTGGCCTGGTGGATCGAAAACGCTCCTGGA
  AGAGACTAAAGGACATTAAGAATATTTTCCCTGCTACCAAGTCTGTCGTCTCAGAGTATGTGGCCGAGCA
  CTGGACAGAGGACAGCTTCTTTGGGTACCAGTACCTTAATGGTATTAACCCAGGCCTGATCCGAAGATGT
  ACACAGATCCCAGACAAATTCCCAGTCACAGATGAAATGGTGGCCCCATTCCTGGGTGAAGGAACATGCC
  TGCAAGCAGAGCTGGAGAGGGGGAATATTTACCTGGCAGACTACCGCATCCTGGATGGCATCCCCACCGT
  GGAGCTCAATGGTCAGCAGCAGCATCACTGTGCCCCGATGTGCTTGCTGCACTTTGGTCCTGATGGCAAC
  ATGATGCCCATCGCCATTCAGCTCAGTCAGACTCCTGGGCCTGATTGTCCTATCTTCCTGCCGAATGATT
  CTGAGTGGGACTGGCTGTTGGCTAAAACGTGGGTGCGCTACGCGGAGTTCTACAGCCACGAGGCCGTGGC
  ACACTTGCTGGAGAGCCACCTCATCGGGGAAGCTTTCTGCTTGGCCCTCCTGAGGAACCTGCCCATGTGC
  CACCCCCTGTATAAGCTTCTCATCCCTCACACTCGGTACAACGTCCAGATCAATAGCATTGGGCGGGCCC
  TCCTTCTCAACAAGGGAGGTCTCTCTGCCAGGGCCATGTCCCTGGGCCTAGAGGGCTTCGCACAGGTGAT
  GGTCCGGGGTCTGTCTGAGCTCACCTACAAAAGCCTCTGCATTCCCAACGACTTTGTGGAGCGCGGGGTC
  CAGGACCTGCCTGGGTATTATTTCCGTGATGACAGCCTGGCGGTGTGGTACGCGATGGAGAGGTACGTGA
  CAGAGATCATCACTTATTATTACCCAAATGACGCAGCCGTAGAGGGCGACCCGGAACTGCAGTGCTGGGT
  ACAGGAGATATTCAAGGAGTGTCTCCTGGGGCGTGAGAGCTCAGGCTTCCCCACCTGCTTGCGAACTATA
  CCTGAGCTGATCGAATACGTCACAATGGTCATGTACACCTGCTCGGCCAGGCATGCAGCTGTCAACTCAG
  GCCAGCTGGAGTACACCTCTTGGATGCCCAACTTCCCATCATCCATGAGGAACCCACCGATGCAGACCAA
  GGGGCTAACTACTCTGCAGACATACATGGACACTCTTCCGGACGTGAAGACCACATGCATTGTGCTCTTG
  GTGCTCTGGACTCTCTGTCGGGAGCCTGATGACAGGCGGCCGCTTGGCCACTTCCCAGACATCCATTTTG
  TGGAGGAGGGTCCTCGGAGGAGTATTGAGGCTTTTCGTCAGAACCTCAACCAGATCTCTCACAACATCCG
  ACAGCGCAATAAGTGCCTCACCCTCCCGTACTACTACCTGGACCCAGTGCTGATTGAGAACAGTATTTCC
  ATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223970 representing NM_009659
  Red=Cloning site Green=Tags(s)
  
  MATYKVKVATGTDFFSGTLDSISLTIVGTQGESHKQRLNHFGRDFATGAVDDYTVQCQQDLGELIIIRLH
  KEPHSFLAKDPWYCNYVQICAPDCRVYHFPAYQWMDGYETLALREATGKITADDTLPILLEHRQEEIRAK
  KDFYHWRVFVPGLPNYVDIPSYHPPPRRCRNPNRPEWDGYIPGFPILINIKATRFLNSNLRFSFVKTASF
  FYRLGPMALAFKLRGLVDRKRSWKRLKDIKNIFPATKSVVSEYVAEHWTEDSFFGYQYLNGINPGLIRRC
  TQIPDKFPVTDEMVAPFLGEGTCLQAELERGNIYLADYRILDGIPTVELNGQQQHHCAPMCLLHFGPDGN
  MMPIAIQLSQTPGPDCPIFLPNDSEWDWLLAKTWVRYAEFYSHEAVAHLLESHLIGEAFCLALLRNLPMC
  HPLYKLLIPHTRYNVQINSIGRALLLNKGGLSARAMSLGLEGFAQVMVRGLSELTYKSLCIPNDFVERGV
  QDLPGYYFRDDSLAVWYAMERYVTEIITYYYPNDAAVEGDPELQCWVQEIFKECLLGRESSGFPTCLRTI
  PELIEYVTMVMYTCSARHAAVNSGQLEYTSWMPNFPSSMRNPPMQTKGLTTLQTYMDTLPDVKTTCIVLL
  VLWTLCREPDDRRPLGHFPDIHFVEEGPRRSIEAFRQNLNQISHNIRQRNKCLTLPYYYLDPVLIENSIS
  I
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009659
• ORF Size: 2103 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009659.2, NP_033789.1
• RefSeq Size: 2347
• RefSeq ORF: 2106
• Locus ID: 11686
• Gene Summary: This gene encodes an enzyme involved in the conversion of arachidonic acid to 12R-hydroxyeicosatetraenoic acid. Mutations in this gene can prevent the formation of the epidermal permeability barrier and cause an ichthyosiform phenotype. [provided by RefSeq, Sep 2015]