Agrn (NM_021604) Mouse Tagged ORF Clone

CAT#: MG224120

Agrn (GFP-tagged) - Mouse agrin (Agrn), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Agrn
• Synonyms: Agrin; nmf380
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224120 representing NM_021604
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGGCGCGCCC
  
  ATGCCTCCTGATGTGTGCAGGGGAATGTTATGTGGCTTTGGTGCTGTGTGTGAACCTAGTGTTGAGGATC
  CAGGCCGGGCCTCCTGTGTGTGCAAGAAGAATGTCTGCCCTGCTATGGTAGCTCCTGTGTGTGGCTCAGA
  TGCTTCCACCTATAGCAACGAGTGTGAGCTACAGCGTGCACAGTGCAACCAGCAACGGCGCATCCGCCTA
  CTCCGCCAAGGGCCATGTGGGTCCCGGGACCCCTGTGCCAATGTGACCTGCAGTTTCGGTAGTACCTGTG
  TACCTTCAGCCGATGGACAGACCGCCTCGTGTCTGTGTCCTACAACCTGCTTTGGGGCCCCTGATGGCAC
  AGTGTGTGGCAGTGATGGTGTTGACTACCCTAGTGAGTGCCAGCTGCTCCGTCATGCCTGTGCCAACCAG
  GAGCACATCTTTAAGAAGTTCGATGGTCCTTGTGACCCCTGCCAGGGCAGCATGTCAGACCTGAATCATA
  TTTGCCGGGTGAACCCACGTACACGGCACCCAGAAATGCTTCTGCGGCCTGAGAACTGCCCCGCCCAACA
  CACACCTATCTGTGGAGATGATGGGGTCACCTATGAAAACGACTGTGTCATGAGCCGTATAGGTGCAGCC
  CGTGGCCTGCTTCTCCAGAAAGTGCGCTCTGGTCAATGCCAGACTCGAGACCAGTGCCCGGAGACCTGCC
  AGTTTAACTCTGTATGCCTGTCCCGCCGCGGCCGTCCCCACTGTTCCTGCGATCGCGTCACCTGTGATGG
  GGCTTACAGGCCAGTGTGTGCCCAGGATGGGCACACGTATGACAATGACTGTTGGCGCCAACAGGCCGAG
  TGTCGACAACAGCAGACCATTCCCCCCAAGCACCAGGGCCCGTGTGACCAGACCCCATCCCCGTGCCGTG
  GAGCGCAGTGTGCATTTGGGGCAACATGCACAGTGAAGAATGGGAAAGCTGTGTGCGAGTGCCAGCGGGT
  GTGCTCGGGCGGCTACGATCCTGTGTGCGGCAGTGATGGTGTCACTTACGGCAGTGTGTGCGAGCTGGAA
  TCCATGGCCTGTACCCTTGGGCGGGAAATCCGAGTGGCCCGCAGAGGACCGTGTGACCGATGTGGGCAGT
  GCCGGTTTGGATCCTTGTGCGAGGTGGAGACTGGACGCTGTGTGTGCCCCTCTGAGTGTGTGGAGTCAGC
  CCAGCCCGTATGTGGCTCTGACGGACACACATATGCTAGTGAATGTGAGCTGCATGTCCACGCCTGTACA
  CACCAGATCAGCCTATACGTGGCCTCAGCCGGACACTGCCAGACCTGTGGAGAAACAGTTTGTACCTTTG
  GGGCTGTGTGCTCAGCTGGACAGTGTGTATGTCCCCGTTGTGAGCACCCCCCACCTGGCCCTGTGTGCGG
  CAGTGATGGCGTCACCTACCTCAGTGCCTGTGAGCTCCGAGAGGCTGCCTGTCAGCAGCAGGTACAAATT
  GAGGAGGCCCGTGCAGGGCCGTGTGAGCCGGCTGAGTGTGGCTCAGGGGGCTCTGGGTCTGGGGAAGACA
  ATGCGTGTGAGCAGGAGCTGTGTCGGCAGCATGGTGGTGTCTGGGATGAGGACTCAGAAGACGGGCCGTG
  TGTCTGTGACTTTAGTTGCCAGAGTGTCCTTAAAAGCCCAGTGTGTGGCTCAGATGGAGTCACCTATAGC
  ACGGAGTGCCATCTGAAGAAGGCCAGATGTGAAGCGCGGCAAGAGCTGTACGTCGCTGCTCAGGGAGCCT
  GCCGGGGCCCTACCTTGGCTCCACTGCTACCTATGGCCTCCCCACACTGTGCCCAAACCCCCTATGGCTG
  CTGCCAGGACAATGTCACTGCTGCCCAGGGTGTGGGCTTGGCTGGCTGTCCCAGCACCTGCCATTGCAAC
  CCACACGGCTCCTATAGCGGCACTTGTGACCCAGTCACAGGGCAGTGCTCCTGCCGACCAGGTGTAGGAG
  GCCTCAGGTGTGATCGCTGTGAGCCTGGCTTCTGGAACTTCCGTGGCATTGTCACCGATGGACATAGTGG
  TTGCACTCCCTGCAGCTGTGACCCTCGGGGTGCTGTAAGAGATGACTGTGAGCAGATGACTGGATTGTGT
  TCCTGTAGACCTGGTGTGGCTGGTCCCAAGTGTGGGCAGTGTCCAGATGGTCAAGCCCTGGGCCATCTTG
  GCTGTGAAGCAGATCCCACAACACCAGTGACTTGTGTGGAAATGCACTGTGAGTTTGGCGCCTCCTGCGT
  AGAGGAGGCTGGTTTTGCCCAGTGTGTCTGCCCAACTCTCACATGTCCAGAGGCTAACTCTACCAAGGTC
  TGTGGATCAGATGGTGTCACATACGGCAATGAATGCCAGCTGAAGACCATTGCCTGCCGCCAGCGTCTGG
  ACATCTCCATTCAGAGTCTTGGTCCATGCCGGGAGAGTGTTGCTCCTGGGGTTTCCCCTACATCTGCATC
  TATGACCACCCCAAGGCATATCCTGAGCAGGACACTGGCGTCTCCCCACAGCAGCCTTCCTCTGTCTCCC
  AGCACTACTGCCCATGATTGGCCCACCCCATTACCCACATCACCTCAGACCGTAGTCGGCACCCCCAGGA
  GCACTGCAGCCACACCCTCTGATGTGGCCAGTCTTGCTACAGCGATCTTCAGGGAATCTGGCAGCACCAA
  CGGCAGTGGCGATGAGGAGCTCAGTGGCGATGAGGAGGCCAGTGGGGGCGGGTCTGGGGGACTTGAGCCC
  CCGGTGGGCAGCGTTGTGGTGACCCACGGGCCACCCATCGAGAGGGCTTCCTGTTACAACTCACCTTTGG
  GCTGCTGCTCAGATGGCAAGACACCCTCACTGGACTCAGAAGGCTCCAACTGTCCAGCTACCAAGGCATT
  CCAGGGCGTGCTGGAGCTTGAGGGGGTCGAGGGACAGGAACTGTTCTACACACCAGAGATGGCTGACCCC
  AAGTCAGAGTTGTTTGGGGAGACTGCAAGGAGCATTGAGAGCACGCTGGACGACCTGTTCCGGAATTCGG
  ATGTTAAGAAGGACTTCTGGAGCATCCGCCTACGGGAACTGGGGCCTGGCAAATTAGTCCGTGCCATTGT
  GGATGTTCACTTTGACCCCACCACAGCCTTCCAGGCACCAGATGTGGGTCAGGCCTTGCTCCAACAGATC
  CAGGTATCCAGGCCGTGGGCCCTGGCAGTGAGGAGGCCTCTGCGGGAGCATGTGCGATTCTTGGACTTTG
  ACTGGTTTCCCACTTTTTTTACGGGAGCTGCAACAGGAACCACAGCTGCTGTGGCCACAGCCAGAGCCAC
  CACTGTGAGCCGACTGTCTGCCTCTTCTGTCACCCCACGAGTCTACCCCAGTTACACCAGCCGGCCTGTT
  GGCAGAACTACGGCACCGCTAACCACTCGCCGGCCACCAACCACTACCGCCAGTATTGACCGACCTCGGA
  CTCCAGGCCCGCAACGGCCCACAAAGTCCTGTGATTCCCAGCCTTGCCTCCACGGAGGTACCTGCCAGGA
  CCTGGATTCTGGCAAGGGTTTCAGCTGCAGCTGTACTGCAGGCAGGGCTGGCACTGTCTGTGAGAAAGTG
  CAACTCCCCTCTGTGCCAGCTTTTAAGGGCCACTCCTTCTTGGCCTTCCCCACCCTCCGAGCCTACCACA
  CGCTGCGTCTGGCACTAGAATTCCGGGCGCTGGAGACAGAGGGACTGCTGCTCTACAATGGCAATGCACG
  TGGCAAAGATTTCCTGGCTCTGGCTCTGTTGGATGGTCATGTACAGTTCAGGTTCGACACGGGCTCAGGG
  CCGGCGGTGCTAACAAGCTTAGTGCCAGTGGAACCGGGACGGTGGCACCGCCTCGAGTTGTCACGGCATT
  GGCGGCAGGGCACACTTTCTGTGGATGGCGAGGCTCCTGTTGTAGGTGAAAGTCCGAGTGGCACTGATGG
  CCTCAACTTGGACACGAAGCTCTATGTGGGTGGTCTCCCAGAAGAACAAGTTGCCACGGTGCTTGATCGG
  ACCTCTGTGGGCATCGGCCTGAAAGGATGCATTCGTATGTTGGACATCAACAACCAGCAGCTGGAGCTGA
  GCGATTGGCAGAGGGCTGTGGTTCAAAGCTCTGGTGTGGGGGAATGCGGAGACCATCCCTGCTCACCTAA
  CCCCTGCCATGGCGGGGCCCTCTGCCAGGCCCTGGAGGCTGGCGTGTTCCTCTGTCAGTGCCCACCTGGC
  CGCTTTGGCCCAACTTGTGCAGATGAAAAGAACCCCTGCCAACCGAACCCCTGCCACGGGTCAGCCCCCT
  GCCATGTGCTTTCCAGGGGTGGGGCCAAGTGTGCGTGCCCCCTGGGACGCAGTGGTTCCTTCTGTGAGAC
  AGTCCTGGAGAATGCTGGCTCCCGGCCCTTCCTGGCTGACTTTAATGGCTTCTCCTACCTGGAACTGAAA
  GGCTTGCACACCTTCGAGAGAGACCTAGGGGAGAAGATGGCGCTGGAGATGGTGTTCTTGGCTCGTGGGC
  CCAGTGGCTTACTCCTCTACAATGGGCAGAAGACGGATGGCAAGGGGGACTTTGTATCCCTGGCCCTGCA
  TAACCGGCACCTAGAGTTCCGCTATGACCTTGGCAAGGGGGCTGCAATCATCAGGAGCAAAGAGCCCATA
  GCCCTGGGCACCTGGGTTAGGGTATTCCTGGAACGAAATGGCCGCAAGGGTGCCCTTCAAGTGGGTGATG
  GGCCCCGTGTGCTAGGGGAATCTCCGGTCCCGCACACCATGCTCAACCTCAAGGAGCCCCTCTATGTGGG
  GGGAGCTCCTGACTTCAGCAAGCTGGCTCGGGGCGCTGCAGTGGCCTCCGGCTTTGATGGTGCCATCCAG
  CTGGTGTCTCTAAGAGGCCATCAGCTGCTGACTCAGGAGCATGTGTTGCGGGCAGTAGATGTAGCGCCTT
  TTGCAGGCCACCCTTGTACCCAGGCCGTGGACAACCCCTGCCTTAATGGGGGCTCCTGTATCCCGAGGGA
  AGCCACTTATGAGTGCCTGTGTCCTGGGGGCTTCTCTGGGCTGCACTGCGAGAAGGGGATAGTTGAGAAG
  TCAGTGGGGGACCTAGAAACACTGGCCTTTGATGGGCGGACCTACATCGAGTACCTCAATGCTGTGACTG
  AGAGTGAGAAAGCGCTGCAGAGCAACCACTTTGAGCTGAGCTTACGCACTGAGGCCACGCAGGGGCTGGT
  GCTGTGGATTGGAAAGGTTGGAGAACGTGCAGACTACATGGCTCTGGCCATTGTGGATGGGCACCTACAA
  CTGAGCTATGACCTAGGCTCCCAGCCAGTTGTGCTGCGCTCCACTGTGAAGGTCAACACCAACCGCTGGC
  TTCGAGTCAGGGCTCACAGGGAGCACAGGGAAGGTTCCCTTCAGGTGGGCAATGAAGCCCCTGTGACTGG
  CTCTTCCCCGCTGGGTGCCACACAATTGGACACAGATGGAGCCCTGTGGCTTGGAGGCCTACAGAAGCTT
  CCTGTGGGGCAGGCTCTCCCCAAGGCCTATGGCACGGGTTTTGTGGGCTGTCTGCGGGACGTGGTAGTGG
  GCCATCGCCAGCTGCATCTGCTGGAGGACGCTGTCACCAAACCAGAGCTAAGACCCTGCCCCACTCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224120 representing NM_021604
  Red=Cloning site Green=Tags(s)
  
  MPPDVCRGMLCGFGAVCEPSVEDPGRASCVCKKNVCPAMVAPVCGSDASTYSNECELQRAQCNQQRRIRL
  LRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLRHACANQ
  EHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGAA
  RGLLLQKVRSGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAE
  CRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELE
  SMACTLGREIRVARRGPCDRCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACT
  HQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQI
  EEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYS
  TECHLKKARCEARQELYVAAQGACRGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCN
  PHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLC
  SCRPGVAGPKCGQCPDGQALGHLGCEADPTTPVTCVEMHCEFGASCVEEAGFAQCVCPTLTCPEANSTKV
  CGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVSPTSASMTTPRHILSRTLASPHSSLPLSP
  STTAHDWPTPLPTSPQTVVGTPRSTAATPSDVASLATAIFRESGSTNGSGDEELSGDEEASGGGSGGLEP
  PVGSVVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADP
  KSELFGETARSIESTLDDLFRNSDVKKDFWSIRLRELGPGKLVRAIVDVHFDPTTAFQAPDVGQALLQQI
  QVSRPWALAVRRPLREHVRFLDFDWFPTFFTGAATGTTAAVATARATTVSRLSASSVTPRVYPSYTSRPV
  GRTTAPLTTRRPPTTTASIDRPRTPGPQRPTKSCDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCEKV
  QLPSVPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGHVQFRFDTGSG
  PAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDR
  TSVGIGLKGCIRMLDINNQQLELSDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPG
  RFGPTCADEKNPCQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELK
  GLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAIIRSKEPI
  ALGTWVRVFLERNGRKGALQVGDGPRVLGESPVPHTMLNLKEPLYVGGAPDFSKLARGAAVASGFDGAIQ
  LVSLRGHQLLTQEHVLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEK
  SVGDLETLAFDGRTYIEYLNAVTESEKALQSNHFELSLRTEATQGLVLWIGKVGERADYMALAIVDGHLQ
  LSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKL
  PVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021604
• ORF Size: 5598 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021604.2, NP_067617.2
• RefSeq Size: 7379
• RefSeq ORF: 6105
• Locus ID: 11603
• Gene Summary: Agrin C-terminal 22 kDa fragment: this released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia. [UniProtKB/Swiss-Prot Function]