Plpp1 (NM_008903) Mouse Tagged ORF Clone

CAT#: MG224342

Ppap2a (GFP-tagged) - Mouse phosphatidic acid phosphatase type 2A (Ppap2a) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Plpp1
• Synonyms: Hic53; Hpic53; LPP-1; LPP1; mPAP; PAP-2a; PAP2-alpha; PAP2a; Ppap2a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224342 representing NM_008903
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGACAAGACGCGGCTGCCGTACGTGGCCCTCGATGTGATTTGCGTGTTGCTGGCTGGATTGCCTT
  TTGCAATTCTTACTTCAAGGCATACCCCCTTCCAGCGAGGAATATTCTGTAATGATGACTCCATCAAGTA
  CCCTTACAAGGAAGACACCATACCTTATGCCTTATTAGGTGGAATAGTCATTCCATTCTGTATTATCGTT
  ATGAGTATTGGAGAATCTCTGTCTGTTTACTTTAATGTCTTGCATTCGAATTCCTTTGTCGGCAATCCCT
  ACATAGCCACCATTTACAAAGCCGTCGGAGCCTTTTTGTTCGGAGTCTCAGCTAGTCAGTCCTTGACTGA
  CATCGCTAAGTATACTATAGGCAGTTTGCGGCCGCACTTCTTGGCTATCTGTAACCCAGACTGGTCAAAA
  ATCAACTGCAGTGATGGCTATATTGAGGACTACATATGTCAAGGGAATGAAGAGAAAGTCAAGGAGGGCA
  GGTTGTCTTTCTACTCGGGACACTCTTCATTCTCTATGTACTGCATGCTGTTTGTCGCACTTTATCTTCA
  AGCCAGGATGAAGGGAGACTGGGCAAGACTCTTACGACCCATGCTCCAGTTTGGGCTCATTGCTTTTTCC
  ATATATGTGGGCCTTTCTCGAGTGTCTGACTACAAACACCACTGGAGTGACGTCACAGTTGGACTCATTC
  AGGGAGCTGCTATGGCTATACTGGTTGCTTTGTATGTATCCGATTTCTTCAAGGACACACATTCTTACAA
  AGAGAGAAAGGAAGAGGATCCACACACGACTCTCCATGAAACCGCCAGTTCACGGAACTACTCAACCAAT
  CACGAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224342 representing NM_008903
  Red=Cloning site Green=Tags(s)
  
  MFDKTRLPYVALDVICVLLAGLPFAILTSRHTPFQRGIFCNDDSIKYPYKEDTIPYALLGGIVIPFCIIV
  MSIGESLSVYFNVLHSNSFVGNPYIATIYKAVGAFLFGVSASQSLTDIAKYTIGSLRPHFLAICNPDWSK
  INCSDGYIEDYICQGNEEKVKEGRLSFYSGHSSFSMYCMLFVALYLQARMKGDWARLLRPMLQFGLIAFS
  IYVGLSRVSDYKHHWSDVTVGLIQGAAMAILVALYVSDFFKDTHSYKERKEEDPHTTLHETASSRNYSTN
  HEP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008903
• ORF Size: 849 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008903.1, NP_032929.1
• RefSeq Size: 1212
• RefSeq ORF: 852
• Locus ID: 19012
• Gene Summary: Broad-specificity phosphohydrolase that dephosphorylates exogenous bioactive glycerolipids and sphingolipids. Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), diacyl glycerol pyrophosphate (DGPP), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA > PA > C-1-P > S-1-P. [UniProtKB/Swiss-Prot Function]