Gucy1b3 (NM_017469) Mouse Tagged ORF Clone

CAT#: MG224437

Gucy1b3 (GFP-tagged) - Mouse guanylate cyclase 1 soluble beta 3 (Gucy1b3) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gucy1b3
• Synonyms: GC-S-beta-1; GCbeta1; Gucy1b3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224437 representing NM_017469
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACGGTTTCGTGAACCATGCCCTGGAGCTGCTGGTGATCCGCAATTATGGTCCCGAGGTGTGGGAAG
  ACATCAAAAAAGAGGCACAGCTGGATGAAGAAGGCCAGTTTCTTGTCAGAATAATATACGATGATTCCAA
  AACATATGACCTGGTGGCTGCTGCAAGCAAAGTCCTCAACCTCAATGCTGGCGAAATCCTGCAGATGTTT
  GGGAAGATGTTTTTCGTCTTCTGCCAGGAGTCTGGCTATGATACCATCTTGCGTGTCCTGGGATCTAATG
  TCAGAGAGTTTTTGCAGAACCTCGATGCCCTGCATGACCACCTCGCCACCATTTACCCAGGGATGCGCGC
  GCCTTCCTTCAGGTGCACCGATGCGGAGAAAGGCAAAGGGCTCATCCTGCACTACTACTCGGAAAGAGAG
  GGGCTTCAGGACATCGTGATCGGGATTATCAAGACTGTTGCTCAACAGATACACGGCACTGAGATAGACA
  TGAAGGTGATTCAGCAAAGAAATGAAGAATGTGATCACACCCAGTTTTTAATTGAAGAAAAGGAATCAAA
  AGAAGAGGATTTTTATGAAGATCTGGATAGGTTTGAAGAGAATGGTACCCAGGAATCACGTATCAGCCCT
  TACACCTTCTGCAAAGCATTTCCTTTTCACATCATATTTGACCGGAACCTAGTGGTCACTCAGTGTGGCA
  ATGCCATCTACAGAGTGCTCCCCCAGCTCCAGCCTGGGAACTGCAGCCTTCTGTCTGTCTTCTCTCTGGT
  CCGCCCTCACATCGACATCAGTTTCCATGGGATTCTTTCACACATCAATACAGTCTTTGTACTGAGAAGC
  AAGGAAGGGTTGCTGGATGTGGAGAAACTTGAGTGTGAGGATGAACTGACTGGAGCAGAGATTAGCTGCT
  TACGTCTCAAAGGCCAAATGATCTACTTACCAGAAGCAGATAGCATCCTCTTCCTCTGTTCACCAAGTGT
  GATGAACCTGGACGACCTAACAAGAAGAGGCCTGTATCTGAGTGACATCCCTCTCCACGATGCTACCCGA
  GACCTGGTTCTTTTGGGAGAACAGTTCCGGGAGGAGTACAAACTGACACAAGAGCTGGAAATCCTCACCG
  ACAGGCTGCAGCTCACACTGAGAGCCTTGGAGGATGAGAAGAAAAAGACAGACACATTGCTGTATTCTGT
  CCTTCCTCCATCTGTTGCCAATGAGCTGAGACACAAGCGCCCAGTGCCTGCCAAAAGATACGACAATGTG
  ACCATCCTCTTCAGCGGCATTGTGGGCTTCAATGCTTTCTGTAGCAAGCATGCATCTGGAGAAGGGGCCA
  TGAAGATTGTCAATCTCCTCAACGATCTCTACACCCGATTTGACACACTGACTGATTCACGAAAAAACCC
  ATTTGTTTACAAGGTGGAAACAGTTGGTGACAAGTATATGACAGTGAGTGGCTTGCCAGAACCTTGTATC
  CACCATGCACGGTCCATTTGCCACCTGGCTTTAGACATGATGGAAATTGCTGGTCAAGTTCAAGTAGATG
  GTGAATCTGTTCAGATAACAATCGGGATCCATACCGGGGAGGTGGTGACAGGTGTGATTGGACAGCGGAT
  GCCTCGGTATTGTCTCTTTGGGAATACCGTCAACCTCACAAGCAGGACAGAAACCACAGGAGAAAAGGGA
  AAGATAAATGTTTCCGAATATACATACAGGTGTCTCATGTCTCCAGAAAACTCGGATCCACTGTTCCATT
  TGGAGCACAGAGGCCCAGTGTCTATGAAGGGCAAGAAGGAACCAATGCAAGTCTGGTTCCTATCCAGGAA
  AAATACAGGCACGGAGGAAACAAATGAGGAGGATGAAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224437 representing NM_017469
  Red=Cloning site Green=Tags(s)
  
  MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYDDSKTYDLVAAASKVLNLNAGEILQMF
  GKMFFVFCQESGYDTILRVLGSNVREFLQNLDALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSERE
  GLQDIVIGIIKTVAQQIHGTEIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISP
  YTFCKAFPFHIIFDRNLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVFVLRS
  KEGLLDVEKLECEDELTGAEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLTRRGLYLSDIPLHDATR
  DLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDTLLYSVLPPSVANELRHKRPVPAKRYDNV
  TILFSGIVGFNAFCSKHASGEGAMKIVNLLNDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCI
  HHARSICHLALDMMEIAGQVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKG
  KINVSEYTYRCLMSPENSDPLFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETNEEDEN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017469
• ORF Size: 1860 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017469.4, NP_059497.1
• RefSeq Size: 3251
• RefSeq ORF: 1863
• Locus ID: 54195