Cyp24a1 (NM_009996) Mouse Tagged ORF Clone

CAT#: MG224484

Cyp24a1 (GFP-tagged) - Mouse cytochrome P450 family 24 subfamily a polypeptide 1 (Cyp24a1) nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyp24a1
• Synonyms: 24-OHase; CP24; Cyp24
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224484 representing NM_009996
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGCCCCATTGACAAAAGGCGCCCCCTGATCGCTTTCCTGCGCCGGCTGCGGGACCTCGGGCAAC
  CCCCAAGGTCCGTGACATCCAAGGCGCACGTGAAGCGTGCGCCAAAAGAGGTGCCCCTCTGCCCGCTGAT
  GACCGACGGTGAGACTCGGAACGTCACCTCCTTACCTGGACCCACCAACTGGCCACTGCTGGGCAGCCTA
  CTGGAGATTTTTTGGAAAGGTGGCCTGAAGAAACAGCACGACACACTGGCAGAGTACCACAAGAAGTATG
  GCCAGATTTTCCGGATGAAGCTGGGCTCCTTCGACTCGGTGCATCTGGGCTCGCCGAGCCTGCTGGAAGC
  TCTGTACCGCACAGAGAGCGCGCATCCCCAGCGGCTAGAGATCAAACCCTGGAAAGCCTATCGGGACCAT
  CGCAACGAAGCCTACGGGCTGATGATCCTGGAAGGACAGGAGTGGCAGCGGGTCCGCAGCGCCTTCCAAA
  AGAAACTCATGAAGCCCGTGGAAATCATGAAGCTGGACAAGAAAATCAATGAGGTCTTGGCTGATTTTAT
  GGGGCAAATCGATGAGCTGCGTGATGAGAGAGGCCGCATCCAAGACTTGTACAGCGAGCTGAACAAATGG
  TCCTTTGAAAGCATCTGCCTTGTGTTATATGAGAAGAGATTCGGGCTCCTTCAAAAGGACACAGAGGAAG
  AAGCCCTGACCTTCATTGCGGCCATCAAAACAATGATGAGCACATTTGGGAAGATGATGGTGACCCCCGT
  GGAGCTGCACAAGCGCCTCAACACCAAAGTGTGGCAAGCGCACACGCTGGCCTGGGACACCATTTTCAAA
  TCAGTCAAGCCCTGCATCGACCACCGCCTAGAGAGATATTCCCAGCAGCCTGGTGCGGATTTCCTTTGTG
  ATATTTATCAGCAAGATCATCTTTCCAAGAAAGAACTGTACGCTGCTGTCACGGAGCTCCAGCTGGCTGC
  AGTGGAGACGACCGCAAACAGCTTGATGTGGATTCTCTACAATCTATCCCGGAATCCCCAAGTGCAACAG
  AGACTTCTCCGGGAAATCCAGAGCGTGCTGCCTGACAACCAGACGCCACGGGCGGAAGATGTGAGGAATA
  TGCCCTATTTAAAGGCCTGTCTAAAGGAGTCCATGAGGCTTACCCCAAGTGTGCCATTCACAACTCGGAC
  CCTTGACAAGCCAACCGTTCTGGGTGAATACACGCTACCCAAAGGAACAGTCTTAACACTAAATACCCAA
  GTGCTGGGCTCTAGCGAAGACAATTTTGAAGATGCTGACAAGTTTAGACCCGAACGCTGGCTTGAAAAGG
  AGAAAAAGATCAACCCCTTCGCTCATCTCCCATTTGGCGTCGGGAAGAGGATGTGCATCGGGCGCCGGCT
  GGCTGAGCTACAGCTCCACTTGGCTCTTTGCTGGATAATCCAAAAATATAACATTGTGGCTACAGACAGT
  GAACCTGTGGAGATGCTGCATCTTGGCATCCTGGTACCTAGCCGGGAGCTGCCCATTGCGTTCTGTCCAC
  GG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224484 representing NM_009996
  Red=Cloning site Green=Tags(s)
  
  MSCPIDKRRPLIAFLRRLRDLGQPPRSVTSKAHVKRAPKEVPLCPLMTDGETRNVTSLPGPTNWPLLGSL
  LEIFWKGGLKKQHDTLAEYHKKYGQIFRMKLGSFDSVHLGSPSLLEALYRTESAHPQRLEIKPWKAYRDH
  RNEAYGLMILEGQEWQRVRSAFQKKLMKPVEIMKLDKKINEVLADFMGQIDELRDERGRIQDLYSELNKW
  SFESICLVLYEKRFGLLQKDTEEEALTFIAAIKTMMSTFGKMMVTPVELHKRLNTKVWQAHTLAWDTIFK
  SVKPCIDHRLERYSQQPGADFLCDIYQQDHLSKKELYAAVTELQLAAVETTANSLMWILYNLSRNPQVQQ
  RLLREIQSVLPDNQTPRAEDVRNMPYLKACLKESMRLTPSVPFTTRTLDKPTVLGEYTLPKGTVLTLNTQ
  VLGSSEDNFEDADKFRPERWLEKEKKINPFAHLPFGVGKRMCIGRRLAELQLHLALCWIIQKYNIVATDS
  EPVEMLHLGILVPSRELPIAFCPR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009996
• ORF Size: 1542 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009996.3, NP_034126.1
• RefSeq Size: 3296
• RefSeq ORF: 1545
• Locus ID: 13081
• Gene Summary: The protein encoded by this gene localizes to the mitochondrion, where it degrades calcitriol to calcitetrol. This gene is upregulated by binding of calcitriol to the upstream regulatory region and to a downstream enhancer region, thereby allowing calcitriol to autoregulate its concentration in the cell. The encoded protein may also play a role in calcium homeostasis. [provided by RefSeq, Aug 2015]