Agl (NM_001081326) Mouse Tagged ORF Clone

CAT#: MG224581

Agl (GFP-tagged) - Mouse amylo-16-glucosidase 4-alpha-glucanotransferase (Agl), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Agl
• Synonyms: 1110061O17Rik; 9430004C13Rik; 9630046L06Rik; AI850929; C77197
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224581 representing NM_001081326
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACACAGTAAGCAGATTCGAATTTTACTACTGAACGAGATGGAGAAGCTAGAGAAGACCCTCTTCA
  GACTTGAACAAGGATTTGAACTACAGTTCCGATTAGGCCCAACTTTACAAGGAAAAGCTGTCACTGTATA
  TACAAATTACCCATTGCCTGGAGAAGCATTTAATCGAGAAAAGTTCCGTTCCTTGGATTGGGAAAACCCA
  ACGGAGAGAGAAGACGATTCTGATAAATACTGTAAACTTCATCTGCAGCAGTCTGGATCTTTTCAGTACT
  ACTTCCTTCAAGGAAATGAGAAAAGTGGAGGAGGTTACATAGTCGTGGATCCTATTTTACGCATTGGAGT
  TGATAACCACGTGTTACCCTTGGACTGTGTTACTCTGCAGACGTTCTTAGCAAAGTGCTTAGGACCCTTC
  GATGAGTGGGAAAGCCGGCTAAGGGTGGCAAAGGAGTCAGGTTACAACATGATTCACTTCACCCCACTGC
  AGACTCTTGGACTGTCCCGGTCATGCTATTCCCTTGCCGATCAGTTAGAGCTAAATCCTGACTTCTCAAG
  GCCTAGTAAAAGATATACCTGGAGTGACGTTGGGCAGCTAGTGGAGAAGCTGAAAAGGGAGTGGAATATT
  CTTTGTATTACCGATGTGGTCTACAATCATACTGCCACTAATAGTAAGTGGATCCTTGAACACCCGGAAT
  CTGCATACAATCTTGTAAATTCTCCACACTTGAAGCCTGCCTGGGTCCTAGACAGAGCACTCTGGCATTT
  CTCCTGTGATGTTGCAGATGGGAAGTACAGAGAAAAAGGAGTCCCTGCTTTGATTGAAAATGACCAACAC
  ATGAATTGCATTCGAAAAATAATTTGGGAGGACATTTTCCCAAGGATTCAACTCTGGGAGTTTTTCCAAG
  TAGATGTTCACAAAGCAGTTGAACAATTTAGAAGACTGCTCTCTCAAGAAAACAGAAGAGTAACTAAGTC
  TGAACCAAAAGAACATCTTAAAATTATTCAGGACCCTGAATACAGGCGGCGAGGCTGCGCTGTAGATATG
  GACACTGCTCTGGCGACTTTTATACCACATGACAATGGGCCAGCTGCAATTGAAGAGTGCTGTAACTGGT
  TCCGTAAGAGACTGGAGGAGTTAAACTCAGAGAAGCACCACCTCACCAGCTGTCACCAGGAACAGGCAGT
  TAATTGTCTTTTGGGAAACGTGTTTTATGAACGACTGGCAGGCCATGGCCCTAAACTAGGACCTGTCACC
  AGGAAGTATCCTTTAGTTACCAGGTATTTTACTTTCCCCTTTGGAGAAATGGCCTTATCCGCAGAGGAAG
  CCCTGATCCACCTCCCAGATAAAGCGTGCTTCCTGATGGCACACAACGGGTGGGTGATGGGCGATGACCC
  GCTCCGGAACTTTGCCGAGCCAGGTTCGGATGTTTATTTAAGGAGAGAACTTATTTGCTGGGGTGACAGT
  GTTAAATTGCGCTATGGGAATAAACCCGAGGACTGCCCTTATCTGTGGGCACACATGAAGAAATACACCG
  AGATAACTGCCACTCACTTCCAGGGAGTTCGTCTGGACAACTGCCACTCCACGCCTCTCCACGTGGCTGA
  GTACATGCTGGATGCTGCTAGGAAATTACAGCCCAATCTTTATGTAGTGGCTGAGCTGTTCACAGGAAGC
  GAAGAGCTGGACAATATCTTCGTTACTAGACTGGGCATCAGTTCCTTAATAAGAGAGGCAATGAGCGCGT
  ATAACAGTCACGAAGAGGGCAGACTGGTCTACCGGTACGGAGGGGAACCTGTCGGCTCCTTCGTTCAGCC
  CTGTTTGAGGCCGCTCATGCCTGCTATCGCGCATGCCCTGTTTATGGACATCACCCACGATAATGAGTGT
  CCGATTGTGCACAGATCAGCCTATGATGCTCTTCCAAGTACCACAGTTGTTTCCATGGCATGCTGTGCCA
  GTGGAAGCACAAGAGGCTACGACGAGCTAGTGCCTCATCAGATTTCAGTGGTTGCAGAGGAGCGATTTTA
  CACTAAGTGGAATCCTGGGGCATCACCGGCAGATACAGGGGATGTTAATGTCCATAGCGGGATTATTGCA
  GCCAGATGTGCCATCAATAGGCTCCATCAAGAGCTTGGAGCCAAGGGTTTCATTCAAGTGTATGTGGATC
  AAGTGGATGAAGACATAGTGGCTGTAACCAGGCACTCCCCGAGCATCCACCAGTCTGTTGTGGCTGTATC
  GAGAACTGCTTTTAGGAATCCCAAGACTTCCTTTTACAGCAAAGAAGTGCCTCAGATGTGCATCCCTGGC
  AAAATTGAAGAGGTAGTTCTGGAAGCTAGAACTATTGAAAGGAACACAAAACCTTATAAGAAAGACGAGA
  ACTCGATCAATGGAATGCCGAACATGACAGTGGAACTTAGAGAACACATCCAGCTTCATGAAAGTAAAAT
  TGTCAGACAAGCTGGAGTTGCCACAAAGGGTCCCAATGAATATATTCAGGAAATAGAATTTGAGAACCTA
  TCTCCAGGAAGTGTTATTATATTCAGAGTTAGTCTCGATCCACACGCTCAAGTTGCTGTGGGGATTCTTC
  GGAACCACCTGACCCAGTTCAGCTCTCACTTTAAGTCCGGAAGCCTTGCTGTTGACAACGCAGATCCTAT
  ATTAAAAATCCCCTTCGCTTCCATTGCCTCTAAATTGACTTTGGCTGAGCTAAACCAGGTACTTTACCGA
  TGTGAATCAGAAGAACAAGAAGACGGTGGCGGTTGCTATGACATACCAAACTGGTCATCACTTAAGTACG
  CAGGACTTCAAGGATTAATGTCCGTGTTGGCAGAAATAAGACCGAAGAATGACCTGGGGCATCCATTCTG
  TGAAAACTTGAGGTCGGGAGACTGGATGATCGACTACGTGAGTGGTCGGCTTATTTCAAGATCAGGAAGT
  ATTGCTGAGGTTGGTAAATGGCTGCAGGCTATGTTCTTCTACCTGAAGCAGATCCCACGCTACCTCATCC
  CCTGCTACTTTGACGCTATATTGATTGGTGCATACACCACTCTGCTGGATGTAGCGTGGAAACAGATGTC
  AAGCTTTGTTCAGAACGGATCCACCTTCGTGAAGCACCTGTCACTGGGTTCTGTCCAGATGTGTGGAGTC
  GGGAAATGCCCTTGTCTGCCGCTGCTTTCTCCCTCGCTCCTGGATGTGCCGTGCAGGTTAAATGAGATCA
  CAAAGGAGAAGGAGCAGTGCTGTGCCTCCTTAGCTGCAGGCTTACCTCACTTTTCTTCCGGTCTTTTTCG
  CTGCTGGGGGAGGGACACTTTCATTGCCCTTCGAGGGATGCTGCTGGTTACTGGACGCTACCTGGAAGCC
  AGGAACATCATTTTAGCGTTTGCTAGTACCCTGCGGCACGGTCTCATCCCTAACCTCCTCGGCGAAGGGA
  CATATGCCAGGTACAACTGCCGGGACGCCGTGTGGTGGTGGCTGCAGTGCATTCAGGACTATTGCAGGAC
  AGTTCCAAACGGCCTGGACATCCTCAAATGCCCGGTGTCCAGGATGTATCCAACAGATGACTCCGCACCC
  TTGCCTGCTGGGACACTGGACCAGCCTTTGTTTGAAGTCATCCAGGAAGCTATGCAGAGGCACATGCAGG
  GCATCCAGTTCCGAGAGAGGAACGCCGGTCCACAGATCGACCGAAACATGAAGGACGAGGGTTTCAACAT
  AACTGCGGGGATTGATGAAGAAACAGGATTTGTTTATGGAGGAAATCGCTTCAACTGTGGCACGTGGATG
  GATAAAATGGGAGAGAGTGACCGAGCTAGGAACAGAGGGATCCCAGCCACTCCAAGAGATGGGTCTGCTG
  TGGAAATCGTGGGCTTGTGTAAATCTGCTGTTCGTTGGTTGCTGGAATTGTCTAAGAAAAATATCTTTCC
  TTATCATGAAGTCAGAGTAAAAAGACATGGAAAGGTTGTGGCAGTCTCGTACGATGAGTGGAACAGAAAA
  ATACAAAACAATTTCGAAAAGCTGTTTCATGTTTCCGAAGACCCTTCAGATCCTAACGAGAAGCATCCGA
  ACCTGGTTCACAAACGCGGCATCTACAAGGACAGCTATGGTGCGTCGAGTCCTTGGTGTGACTATCAGCT
  CAGGCCCAACTTCACCATAGCAATGGTCGTGGCCCCTGAACTCTTTACTGCTGAGAAGGCTTGGAAGGCT
  TTGGAAATTGCAGAAAAAAAATTGCTTGGTCCCCTTGGCATGAAAACTTTAGACCCAGATGACATGGTTT
  ACTGTGGAGTTTATGATAATGCCTTAGACAATGACAACTACAACCTTGCTAGAGGCTTCAATTACCACCA
  AGGACCTGAGTGGCTGTGGCCAATTGGGTATTTTCTTCGTGCAAAGTTGTATTTTTCCAAAATGATGGGT
  CCAGAGACTGCTGCGAAGACTGTATTTTTGGTTAAAAATGTTCTTTCTAGACATTATGTTCATCTTGAGA
  GATCCCCATGGAAAGGCCTTCCGGAGCTCACCAATGAGAACGGCCAGTACTGCCCTTTCAGCTGTGAGAC
  ACAGGCGTGGTCAATGGCTGTTGTTCTGGAAACCCTCTATGATTTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224581 representing NM_001081326
  Red=Cloning site Green=Tags(s)
  
  MEHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKAVTVYTNYPLPGEAFNREKFRSLDWENP
  TEREDDSDKYCKLHLQQSGSFQYYFLQGNEKSGGGYIVVDPILRIGVDNHVLPLDCVTLQTFLAKCLGPF
  DEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPSKRYTWSDVGQLVEKLKREWNI
  LCITDVVYNHTATNSKWILEHPESAYNLVNSPHLKPAWVLDRALWHFSCDVADGKYREKGVPALIENDQH
  MNCIRKIIWEDIFPRIQLWEFFQVDVHKAVEQFRRLLSQENRRVTKSEPKEHLKIIQDPEYRRRGCAVDM
  DTALATFIPHDNGPAAIEECCNWFRKRLEELNSEKHHLTSCHQEQAVNCLLGNVFYERLAGHGPKLGPVT
  RKYPLVTRYFTFPFGEMALSAEEALIHLPDKACFLMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDS
  VKLRYGNKPEDCPYLWAHMKKYTEITATHFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGS
  EELDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNEC
  PIVHRSAYDALPSTTVVSMACCASGSTRGYDELVPHQISVVAEERFYTKWNPGASPADTGDVNVHSGIIA
  ARCAINRLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKEVPQMCIPG
  KIEEVVLEARTIERNTKPYKKDENSINGMPNMTVELREHIQLHESKIVRQAGVATKGPNEYIQEIEFENL
  SPGSVIIFRVSLDPHAQVAVGILRNHLTQFSSHFKSGSLAVDNADPILKIPFASIASKLTLAELNQVLYR
  CESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCENLRSGDWMIDYVSGRLISRSGS
  IAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDVAWKQMSSFVQNGSTFVKHLSLGSVQMCGV
  GKCPCLPLLSPSLLDVPCRLNEITKEKEQCCASLAAGLPHFSSGLFRCWGRDTFIALRGMLLVTGRYLEA
  RNIILAFASTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCRTVPNGLDILKCPVSRMYPTDDSAP
  LPAGTLDQPLFEVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFNITAGIDEETGFVYGGNRFNCGTWM
  DKMGESDRARNRGIPATPRDGSAVEIVGLCKSAVRWLLELSKKNIFPYHEVRVKRHGKVVAVSYDEWNRK
  IQNNFEKLFHVSEDPSDPNEKHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKA
  LEIAEKKLLGPLGMKTLDPDDMVYCGVYDNALDNDNYNLARGFNYHQGPEWLWPIGYFLRAKLYFSKMMG
  PETAAKTVFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSMAVVLETLYDL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081326
• ORF Size: 4596 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001081326.1, NP_001074795.1
• RefSeq Size: 9625
• RefSeq ORF: 4599
• Locus ID: 77559