Wnt10b (NM_011718) Mouse Tagged ORF Clone

CAT#: MG224739

Wnt10b (GFP-tagged) - Mouse wingless related MMTV integration site 10b (Wnt10b), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wnt10b
• Synonyms: Wnt12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224739 representing NM_011718
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAGGAGCCCCGGTCTCGGCCTCCGCCCTTAGGCCTCGCGGGTCTCCTGTTCTTGGCTTTGTTCA
  GTCGGGCTCTAAGCAATGAGATTCTGGGCCTTAAACTTCCCGGTGAGCCGCCGCTGACGGCCAACACCGT
  GTGCTTGACCCTGTCCGGACTGAGTAAGCGACAGCTGGGGCTGTGCCTGCGCAGCCCCGACGTGACGGCG
  TCGGCGCTCCAGGGGCTGCACATCGCCGTTCACGAGTGTCAGCACCAGCTGCGCGACCAGCGCTGGAACT
  GCTCGGCACTGGAGGGCGGCGGCCGGCTGCCGCACCACAGCGCCATCCTCAAGCGCGGTTTCCGTGAGAG
  TGCTTTCTCCTTCTCCATGCTGGCTGCTGGGGTCATGCATGCTGTTGCCACAGCCTGCAGCCTGGGCAAG
  CTGGTGAGCTGCGGCTGCGGATGGAAGGGTAGTGGTGAGCAAGACCGGCTTAGAGCCAAGCTGCTGCAGC
  TTCAGGCACTGTCTCGGGGCAAGACTTTCCCCATCTCCCAGCCCAGCCCTGTTCCTGGCTCAGTCCCCAG
  CCCCGGCCCCCAGGACACGTGGGAATGGGGTGGCTGTAACCACGACATGGACTTCGGAGAGAAGTTCTCT
  CGGGATTTCTTGGATTCCAGGGAGGCTCCCCGGGACATCCAGGCGAGAATGCGGATCCACAACAACAGGG
  TGGGACGCCAGGTGGTAACGGAAAACCTGAAGCGGAAGTGCAAATGCCATGGAACGTCAGGCAGCTGCCA
  ATTCAAGACCTGTTGGAGGGCAGCGCCAGAGTTCCGGGCCATCGGGGCAGCACTGAGGGAGCGGCTGAGC
  AGAGCCATCTTTATCGATACCCACAACCGCAACTCTGGAGCGTTCCAGCCCCGCCTACGTCCGCGGCGCC
  TCTCTGGAGAGCTGGTTTACTTTGAGAAGTCTCCTGACTTCTGCGAGCGAGACCCTACTCTGGGCTCCCC
  AGGCACGAGAGGCCGGGCTTGCAACAAGACCAGCCGCCTCTTGGATGGCTGTGGCAGCCTGTGCTGTGGC
  CGTGGGCACAACGTGCTCCGGCAGACGCGAGTGGAGCGCTGCCACTGTCGTTTCCACTGGTGCTGTTATG
  TGCTGTGTGATGAGTGTAAAGTCACAGAGTGGGTCAATGTGTGTAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224739 representing NM_011718
  Red=Cloning site Green=Tags(s)
  
  MLEEPRSRPPPLGLAGLLFLALFSRALSNEILGLKLPGEPPLTANTVCLTLSGLSKRQLGLCLRSPDVTA
  SALQGLHIAVHECQHQLRDQRWNCSALEGGGRLPHHSAILKRGFRESAFSFSMLAAGVMHAVATACSLGK
  LVSCGCGWKGSGEQDRLRAKLLQLQALSRGKTFPISQPSPVPGSVPSPGPQDTWEWGGCNHDMDFGEKFS
  RDFLDSREAPRDIQARMRIHNNRVGRQVVTENLKRKCKCHGTSGSCQFKTCWRAAPEFRAIGAALRERLS
  RAIFIDTHNRNSGAFQPRLRPRRLSGELVYFEKSPDFCERDPTLGSPGTRGRACNKTSRLLDGCGSLCCG
  RGHNVLRQTRVERCHCRFHWCCYVLCDECKVTEWVNVCK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011718
• ORF Size: 1167 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_011718.2, NP_035848.1
• RefSeq Size: 2272
• RefSeq ORF: 1170
• Locus ID: 22410