Pde4b (NM_019840) Mouse Tagged ORF Clone

CAT#: MG224769

Pde4b (GFP-tagged) - Mouse phosphodiesterase 4B cAMP specific (Pde4b) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pde4b
• Synonyms: Dpde4; dunce; R74983
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224769 representing NM_019840
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCAAAAAATTCTCCAAAAGAATTTACTGCTTCGGAATCTGAGGTTTGCATAAAGACTTTCAAGG
  AGCAGATGCGCTTGGAACTTGAGCTTCCAAAGCTACCAGGAAACAGACCTACATCTCCCAAAATTTCTCC
  ACGCAGTTCACCAAGGAATTCACCATGCTTTTTCAGAAAGTTGCTGGTGAATAAAAGCATCCGACAGCGG
  CGTCGCTTCACGGTGGCTCATACATGCTTTGATGTGGAAAATGGCCCTTCTCCAGGTCGGAGCCCACTGG
  ACCCTCAAGCCGGCTCTTCGTCGGGACTGGTACTTCATGCCGCCTTTCCTGGGCACAGCCAGCGCAGGGA
  GTCGTTCCTCTACAGATCTGACAGCGACTATGACTTGTCACCAAAAGCGATGTCCAGGAACTCATCACTT
  CCCAGTGAGCAACACGGCGATGACCTGATTGTCACTCCTTTTGCCCAGGTTCTTGCCAGCTTGCGAAGTG
  TAAGAAACAACTTCACCCTGCTGACGAACCTTCATGGAGCGCCGAACAAGAGGTCACCAGCGGCTAGTCA
  GGCTCCAGTCTCCAGAGTCAGCCTGCAAGAAGAATCATATCAGAAACTAGCAATGGAGACGCTGGAGGAA
  CTAGACTGGTGCCTAGACCAGCTAGAGACCATCCAGACCTACCGCTCTGTCAGCGAGATGGCTTCAAACA
  AGTTCAAAAGGATGCTGAACCGGGAGCTGACACACCTCTCAGAGATGAGCAGATCAGGGAACCAGGTGTC
  TGAGTACATTTCAAACACGTTCTTAGACAAGCAGAACGATGTGGAAATCCCATCTCCCACGCAGAAGGAC
  AGGGAGAAGAAGAAGAAGCAGCAGCTCATGACCCAGATAAGTGGAGTGAAGAAACTGATGCACAGCTCAA
  GCCTGAACAACACAAGCATCTCACGCTTCGGAGTCAACACGGAAAATGAGGATCATCTAGCCAAGGAGCT
  GGAAGACCTGAACAAATGGGGCCTTAACATCTTCAATGTGGCTGGGTACTCACATAATCGGCCCCTTACG
  TGCATCATGTATGCAATATTCCAGGAAAGAGACCTTCTGAAGACGTTTAAAATCTCATCTGACACCTTTG
  TAACCTACATGATGACTTTAGAAGACCATTACCATTCTGATGTGGCATATCACAACAGCCTGCATGCTGC
  TGACGTGGCCCAGTCAACTCACGTTCTCCTTTCTACGCCGGCACTGGATGCTGTCTTCACAGACCTGGAA
  ATCCTGGCTGCCATTTTTGCAGCTGCCATCCATGATGTCGATCATCCTGGAGTCTCCAATCAGTTTCTCA
  TCAATACAAATTCTGAACTTGCTTTGATGTATAATGATGAATCTGTTCTGGAAAACCATCACCTTGCTGT
  GGGATTCAAATTGCTACAAGAGGAACACTGCGACATCTTTCAGAATCTTACCAAGAAGCAACGCCAGACA
  CTCAGGAAAATGGTGATTGACATGGTGTTGGCAACTGATATGTCCAAACACATGAGCCTCCTGGCAGACC
  TTAAAACAATGGTAGAAACCAAGAAGGTGACAAGCTCCGGTGTTCTCCTCCTGGACAACTATACTGACCG
  GATACAGGTTCTTCGCAACATGGTACACTGTGCAGACCTGAGCAACCCCACCAAGTCCTTGGAATTGTAT
  CGGCAATGGACCGATCGTATCATGGAGGAGTTTTTCCAGCAGGGAGACAAAGAACGGGAGAGGGGAATGG
  AGATTAGCCCAATGTGTGATAAGCACACAGCTTCTGTGGAAAAATCCCAGGTTGGTTTCATTGACTACAT
  TGTCCATCCACTGTGGGAGACCTGGGCAGACCTGGTTCAACCGGATGCTCAAGATATTCTGGATACACTA
  GAAGATAACAGGAACTGGTACCAGAGTATGATACCCCAGAGCCCTTCCCCGCCACTGGATGAGAGGAGCA
  GGGACTGCCAAGGCCTGATGGAGAAGTTTCAGTTTGAACTGACCCTTGAGGAAGAGGATTCTGAGGGACC
  GGAAAAGGAGGGAGAAGGCCACAGCTATTTCAGCAGCACAAAGACGCTTTGTGTGATTGATCCAGAGAAC
  AGGGATTCTCTGGAAGAGACTGACATAGACATTGCAACAGAAGACAAGTCTCCGATCGACACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224769 representing NM_019840
  Red=Cloning site Green=Tags(s)
  
  MTAKNSPKEFTASESEVCIKTFKEQMRLELELPKLPGNRPTSPKISPRSSPRNSPCFFRKLLVNKSIRQR
  RRFTVAHTCFDVENGPSPGRSPLDPQAGSSSGLVLHAAFPGHSQRRESFLYRSDSDYDLSPKAMSRNSSL
  PSEQHGDDLIVTPFAQVLASLRSVRNNFTLLTNLHGAPNKRSPAASQAPVSRVSLQEESYQKLAMETLEE
  LDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQNDVEIPSPTQKD
  REKKKKQQLMTQISGVKKLMHSSSLNNTSISRFGVNTENEDHLAKELEDLNKWGLNIFNVAGYSHNRPLT
  CIMYAIFQERDLLKTFKISSDTFVTYMMTLEDHYHSDVAYHNSLHAADVAQSTHVLLSTPALDAVFTDLE
  ILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFQNLTKKQRQT
  LRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELY
  RQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTL
  EDNRNWYQSMIPQSPSPPLDERSRDCQGLMEKFQFELTLEEEDSEGPEKEGEGHSYFSSTKTLCVIDPEN
  RDSLEETDIDIATEDKSPIDT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019840
• ORF Size: 2163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019840.2, NP_062814.2
• RefSeq Size: 4371
• RefSeq ORF: 2166
• Locus ID: 18578