Use1 (NM_025917) Mouse Tagged ORF Clone

CAT#: MG224938

Use1 (GFP-tagged) - Mouse unconventional SNARE in the ER 1 homolog (S. cerevisiae) (Use1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Use1
• Synonyms: 2010315L10Rik; 5730403H22Rik; AV002165; D12; Ed2; p31; Q-snare
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224938 representing NM_025917
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGGCGGAAGGAGCCTATCATCGCCCGCTGGCGACGTCGCGGTTGGAGTTGAACCTGGTACGGC
  TGCTTTGTCGCTGTGAGTCGATGGCGGCAGAAAAACGAGAACCGGACGAGTGGCGACTGGAGAAGTATGT
  GGGTGCCCTGGAAGACATGCTGCAGGCCCTGAAAGTTCAAGCAAGTAAACCGGCCTCAGAAGTGATCAGC
  GAGTACTCCCGCAAAGTGGACTTTCTGAAGGGCATGCTGCAGGCCGAGAAGTTGACCTCCTCCTCTGAAA
  AAGCTCTAGCTAACCAGTTCCTGGCTCCTGGCCGCGTGCCCACCACAGCCAAGGAGCGGGTGCCTGCCAC
  CAAGACTGTACATTTGCAGTCTAGGGCACGTTACACCAGTGAGATGCGGAGTGAGCTGTTGGGCATGGAA
  CCATCTGGAGAATGTGAGGTGGACATGAGGAAACGAGCTGCGAAGGGGTCCAGACCTGCAGATGAGAGGC
  AGTCAGCATCCGAGCTAGACCTCGTCCTCCAGAGGCACCAGGGCCTCCAGGAAAAGCTGGCTGAGGAGAT
  GCTAGGCCTGGCCCGCAGCCTCAAGACCAACACACTGGCTGCCCAGAGTGTCATCAAGAAGGACAATCAG
  ACCCTGTCACATTCACTCAAGATGGCAGACCAGAACCTGGAGAAGCTGAAGCTGGAGTCGGAGAGATTGG
  AGCAGCATGCACAGAAGTCAGTGAACTGGCTGCTGTGGGCCATGCTCATTGTCGTGTGCTTCGTGTTCAT
  CAGCATGATCCTGTTTATCCGAATCATGCCCAGGCTGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224938 representing NM_025917
  Red=Cloning site Green=Tags(s)
  
  MAQAEGAYHRPLATSRLELNLVRLLCRCESMAAEKREPDEWRLEKYVGALEDMLQALKVQASKPASEVIS
  EYSRKVDFLKGMLQAEKLTSSSEKALANQFLAPGRVPTTAKERVPATKTVHLQSRARYTSEMRSELLGME
  PSGECEVDMRKRAAKGSRPADERQSASELDLVLQRHQGLQEKLAEEMLGLARSLKTNTLAAQSVIKKDNQ
  TLSHSLKMADQNLEKLKLESERLEQHAQKSVNWLLWAMLIVVCFVFISMILFIRIMPRLK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025917
• ORF Size: 810 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_025917.4, NP_080193.1
• RefSeq Size: 874
• RefSeq ORF: 813
• Locus ID: 67023