Cass4 (NM_001033538) Mouse Tagged ORF Clone

CAT#: MG224996

Cass4 (GFP-tagged) - Mouse Cas scaffolding protein family member 4 (Cass4) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cass4
• Synonyms: F730031O20Rik; RP23-228E2.3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224996 representing NM_001033538
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGGCACCAGCATCAGAGAGGGTGCGCCCAAGACCCTGTTGGCCAGGGCACTCTACGACAACCATG
  CTGATTGCTCCGACGAGCTTGCTTTCTCCAGAGGGGACATCCTGACCATCGTGGAACAAAATGTGCCAGA
  AAGCGAGGGCTGGTGGAGGTGTCTGCTCCACGGGAGGCAAGGCCTGGCTCCAGCCAACCGCCTCCAAGTC
  CTCAGAGAGACCCCTGCAGACAGACCCTGTCCCCTGCTCCCGAGAGGCCCAGACACAGATCTCACCAGCT
  CCGGGGCGCCTTACCAGGTGCAGGACTTGATCTCCCCGCCACCCCAGGGACCTGTGTATGAACCAATGAG
  GAGCTGGGTGGAGGGGCCGTCACCAGCGACTGCCCAAGTCTACGAATTGCCTGAGTCACCTTCCAGTGCC
  AGGATCATCTGTGAAAAGACGCTGAGCTTCCCCAAGCAGGCCCTCTCTGTGCTTCCCAGACCGACGAGGG
  CCTCGTTGCCGACACTTCCTTCCCAGGTGTATGACGTGCCTGTCCAGAGGCAGGGCTTCTCAACCCTTGA
  GAGGCTGGAGAAGCAGCAGTTCTATGACATACCCACCAGCTCCCAGAAGGCTCTGCTCCACTCCTCCACC
  AGCCAGGGACGGGATGTTACATTGGCACCGACAATGGCCTTCAGACAAGGTGGTGGCTATAACCCGTTAT
  CCAGCCCTCAGAAGTCAGAACGGATTCATGACACTCCGGTGTTGCTGGAAAAGGCCGATGTCAGAAATGT
  GTCTATGACCAGCTTCACCAAAGACTCAGGTTCCAGAGCCATTCCAGGCTCCTCTGCTGTCCACACTGGA
  GCTGTGGCCCTCTCCCCACAGCTGGGGAACACTGTGCAGAGAAAAAACAGCCTCCCAGAAGAACCTACCT
  ATGCCTTCCCAACGTCCAGGGACCCACTGCCCTCTGATGCAGGTGGCAGCTACAAGGTCCCTTCCAGGTT
  TCTTATTCCCAGAGTGGAGCAACAGAACACCATGCCTAACATTTATGACACCCCTAAAGCCATGCAGGGT
  GTGTCTCATAACGCCCCTAAAGCCATGCAGGGTGTGTCTCTGGCTGGAAAGGAGCTGGAGAGAGGCAGAG
  AGGCTCCAGAAAATTCCCCCTGGATCTCCGGACAGACAAGTTTCCTGTCTCCTGACTCGGACCGATTATC
  TGTTGCCAGCTCAGACAGCAGGGCTAGTGTGGTGTCTTCCTGCTCCTCCATATCCATGGACTCCTCCTCT
  GGCTCCTCCTCAGAGGACTCCGTCAAGGAGCTATGGATGGACGTGGACTTTGCAAAGGAGACAGCAGTGT
  CTCTGCAACACAAAGTGGCCAGCTCGGCGGCGGGCCTGCTGCTCTTTGTAAGCAGGACTTGGAGGTTCAA
  AGACTCCTTGGAAACCAACATCCACAGAATCCGCAGGGCCGCTGACCACGTAGAGGAATCTGTAAGAGAA
  TTTCTGGATTTTGCCCAAGGGGTTGGTGGGACCGCTTGCAATCTCACAGACAGCTACCTTCAGGCTAGAA
  TTAGAGATCAGCTCCAGACAATCTCTAGCTCCTACCAGACACTGCTGGATGCCAAGGGAAGCTTGGACCG
  CTGCAATTGGTCCCTGGAAGTTCTTGTGACAGACAAAGTCCAAAACAGCCTGGATGACCTGGAGAGGTTT
  GTCGCCACTGCACGAATAGTTCCAGAAGACGTCAAGAGGTTTACCTCCATCGTCATTGCCAATGGGAAGC
  TCCTTTTTAAACAGAACTGTGAGAAAGGAGAGATGGACTTGAAGTGTGAGAGATGCATCCGGCCTCCCCA
  AAGAGAAACTGAGTCCTACCAAGAGAGCAGCCCCTTCGACAGGCAACCGACAACTGAACACTCCTTTGAA
  CTAGCAAGGAAAAACAGAGTGAATGTCTGTTGGCAGGTAAGTCAGGGTGACAAACAGTACACAGGGGACT
  TCATCTGTTACCTGAACTGGTCATCACTGAGGCAGATGCTGGCATCATGTGCCTGTAGAAATGTGCACTT
  GCACGTTCATGGGGAAGCCATAGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224996 representing NM_001033538
  Red=Cloning site Green=Tags(s)
  
  MRGTSIREGAPKTLLARALYDNHADCSDELAFSRGDILTIVEQNVPESEGWWRCLLHGRQGLAPANRLQV
  LRETPADRPCPLLPRGPDTDLTSSGAPYQVQDLISPPPQGPVYEPMRSWVEGPSPATAQVYELPESPSSA
  RIICEKTLSFPKQALSVLPRPTRASLPTLPSQVYDVPVQRQGFSTLERLEKQQFYDIPTSSQKALLHSST
  SQGRDVTLAPTMAFRQGGGYNPLSSPQKSERIHDTPVLLEKADVRNVSMTSFTKDSGSRAIPGSSAVHTG
  AVALSPQLGNTVQRKNSLPEEPTYAFPTSRDPLPSDAGGSYKVPSRFLIPRVEQQNTMPNIYDTPKAMQG
  VSHNAPKAMQGVSLAGKELERGREAPENSPWISGQTSFLSPDSDRLSVASSDSRASVVSSCSSISMDSSS
  GSSSEDSVKELWMDVDFAKETAVSLQHKVASSAAGLLLFVSRTWRFKDSLETNIHRIRRAADHVEESVRE
  FLDFAQGVGGTACNLTDSYLQARIRDQLQTISSSYQTLLDAKGSLDRCNWSLEVLVTDKVQNSLDDLERF
  VATARIVPEDVKRFTSIVIANGKLLFKQNCEKGEMDLKCERCIRPPQRETESYQESSPFDRQPTTEHSFE
  LARKNRVNVCWQVSQGDKQYTGDFICYLNWSSLRQMLASCACRNVHLHVHGEAIG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033538
• ORF Size: 2055 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033538.2, NP_001028710.1
• RefSeq Size: 3731 bp
• RefSeq ORF: 2058 bp
• Locus ID: 320664
• Cytogenetics: 2 H3