Rasgrp4 (NM_145149) Mouse Tagged ORF Clone

CAT#: MG225103

Rasgrp4 (GFP-tagged) - Mouse RAS guanyl releasing protein 4 (Rasgrp4) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rasgrp4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225103 representing NM_145149
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGGAAAGACATCAAAAGGAAGTCTCATCAGGAATGCTCTGGGAAGGCAGGAGGGCGGGGTCGGT
  CACGTCAGGCCCGCCGCCACAAGACGTGCCCCACCCCCCGGGAAATCAGCAAGGTCATGGCGTCCATGAA
  TCTGGGAGTGCTGAGTGAGAGCAGCTGCAGCGAAGATGAGCTATTGGAGGAATGTATCCGCTGCTTTGAC
  TCGGCTGGCAGCCTGCGCCGAGGGGACCACATTCTCAAGATGGTGCTCACGATGCACAGCTGGGTGCTGC
  CATCCTCAGAGCTCGCTGCCCGTCTGCTGACTTCGTATCAGAAGGCTGCCAAAGATGCCCAAGAGCTAAG
  ACAGCTACAGATCTGTTACTTGGTCAGGTACTGGCTGACCCATCACCATGAGGCAGTGCACCAGGAACCC
  CAGCTGGAAGCAGTCATAAGCCGGTTTTGGACCACTGTCGCTCAGGAGGGCAACATGGCCCAAAGGAGCC
  TGGGAGATGCCTCCAGCCTGCTAAGTCCTGGAGGGCCCGGTCCCCCACCTCCCATGAGCAGCCCAGGCCT
  GGGCAAGAAGCGGAAAGTGTCATTGCTATTCGATCACCTGGAGACAGAGGAGCTGGCACAACACCTCACT
  TACCTGGAGTTCCGGTCTTTCCAGGCTATCACACCCCAAGACCTGCGGGGCTATGTTTTGCAGGGTTCAG
  TGAGAGGTTGTCCAGCTCTGGAAGGTTCTGTGGGTCTGAGCAACAGTGTGTCCCGCTGGGTGCAGGTCAT
  GGTGCTGAGTCGTCCTGGGCCTGCACAACGTGCCCAGGTCCTGGACAAATTCATTCGTGTGGCACAGAGG
  CTTCACCAGCTGCAGAATTTCAACACACTGATGGCGGTCACGGGGGGTCTGTGTCATAGCGCCATCTCCA
  GACTCAAGGACTCCCACGTTCATCTGAGCCCTGACAGCACAAAGGCCCTGCTGGAGCTGACAGAGCTCCT
  CTCATCCCACAACAACTATGCCCACTACCGCCGCACCTGGGCCGGCTGTACTGGCTTCCGGCTGCCAGTA
  CTGGGTGTGCACCTCAAGGACCTGGTATCTCTATATGAGGCTCATCCAGACAGATTGCCAGATGGCCGCC
  TGCACCTACCCAAGCTGAATAGCCTCTATCTACGGCTGCAGGAGCTGATGGCGCTCCAGGGACAGCATCC
  TCCCTGCAGTGCCAATGAGGACCTGCTGCACCTGCTGACGCTCTCCCTGGATCTCTTCTACACGGAAGAT
  GAGATCTACGAGCTTTCTTATGCCCGGGAACCACGCTGTCCCAAGAGTCTGCCACCGTCCCCCTTCAAAG
  CACCAGTGGTGGTAGAGTGGGCCCAGGGTGTGACACCAAAGCCAGACAGCGTGACTCTGGGTCAGCATGT
  GGAACAGCTGGTGGAGTCCGTGTTCAAGAACTACGACCCAGAAGGCCGTGGCTCCATCTCTCTGGAGGAC
  TTTGAGCGGCTGTCAGGCAACTTCCCGTTCGCCTGCCATGGGCTTCACCCACCTCCCCGCCATGGGAGTG
  GCTCCTTCAGCAGAGAGGAGCTGACCAAGTACCTGCTCCATGCCAGTGCCATCTGCTCCAAGCTGGGCCT
  GGCCTTCCTGCACGCCTTCCAGGAGGTCACCTTCCGAAAGCCCACGTTCTGTCACAGCTGCAGCGGCTTC
  CTCTGGGGGGTCACCAAGCAAGGCTATCGCTGTCGGGACTGTGGGCTGTGTTGTCACAGACACTGCAGGG
  ATCAAGTGAGAGTGGAGTGTAAGAAGAGGCCAGAGACTAAGGGTGACCCGGGCCCCCCAGGTGCACCTGT
  GCCAGCCACATCACTTCCTCCTGCCAACTGTGGCTCAGAGGAAAGTCTCTCCTATACACTCTCCCCGGAT
  CCGGAGTCTGGTTGCCACCTTCGCCATGCTTGGACCCAGACGGAATCCTCACACTCTTCCTGGGAGCCAG
  AGGTGGTGCCCTGCCCAGCACGGGTCTTACCATCCAGAGCTTCCTCGAAGCCCAGCGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225103 representing NM_145149
  Red=Cloning site Green=Tags(s)
  
  MNRKDIKRKSHQECSGKAGGRGRSRQARRHKTCPTPREISKVMASMNLGVLSESSCSEDELLEECIRCFD
  SAGSLRRGDHILKMVLTMHSWVLPSSELAARLLTSYQKAAKDAQELRQLQICYLVRYWLTHHHEAVHQEP
  QLEAVISRFWTTVAQEGNMAQRSLGDASSLLSPGGPGPPPPMSSPGLGKKRKVSLLFDHLETEELAQHLT
  YLEFRSFQAITPQDLRGYVLQGSVRGCPALEGSVGLSNSVSRWVQVMVLSRPGPAQRAQVLDKFIRVAQR
  LHQLQNFNTLMAVTGGLCHSAISRLKDSHVHLSPDSTKALLELTELLSSHNNYAHYRRTWAGCTGFRLPV
  LGVHLKDLVSLYEAHPDRLPDGRLHLPKLNSLYLRLQELMALQGQHPPCSANEDLLHLLTLSLDLFYTED
  EIYELSYAREPRCPKSLPPSPFKAPVVVEWAQGVTPKPDSVTLGQHVEQLVESVFKNYDPEGRGSISLED
  FERLSGNFPFACHGLHPPPRHGSGSFSREELTKYLLHASAICSKLGLAFLHAFQEVTFRKPTFCHSCSGF
  LWGVTKQGYRCRDCGLCCHRHCRDQVRVECKKRPETKGDPGPPGAPVPATSLPPANCGSEESLSYTLSPD
  PESGCHLRHAWTQTESSHSSWEPEVVPCPARVLPSRASSKPSV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145149
• ORF Size: 2019 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_145149.4, NP_660131.2
• RefSeq Size: 4621
• RefSeq ORF: 2022
• Locus ID: 233046