Cldn20 (NM_001101560) Mouse Tagged ORF Clone

CAT#: MG225233

Cldn20 (GFP-tagged) - Mouse claudin 20 (Cldn20), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cldn20
• Synonyms: EG621628
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225233 representing NM_001101560
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGCAGGTCTCCAGCTCCTTGCTTTCATCCTGGCCGTGTCTGGGGTGTCTGGAGTACTCGCTG
  CCACTCTGCTGCCAAACTGGAAGGTTAATGCATACGCAGGACCCAACATTGTAACTGCTGTTGTCCAGGT
  GCAGGGGTTGTGGGTGGACTGCACGTGGTACAGCACTGGGATGTTTAGCTGCACTCTAAAATACTCCATT
  CTGTCGCTCCCCGTCCATGTGCAGACTGCGAGGGCCACCATGGTCCTGGCCTGTGTTCTGTCTGCTTGGG
  GGATTTGTACTTCGATAGCAGGAATGAACTGCACTCACTTAGGAGGAGACACACACACCAAGAGTAAAAT
  TTCCTTTGCTGGAGGAGTCTGCTTCATCACTGCAGGGATCTCTGGTTTAATCCCAACAGTGTGGTACACC
  AAGGAGATCATAGCGAACTTTCTGGATCTGACCATTCCGGAAAGCCACAAATATGAACCTGGAGGAGCCC
  TGTACATTGGGTTTATTTCGGCGATGCTACTACTTATCTCTGGTGTTATTTTCTGCACTTCTTATATACA
  GAAGAACCAAGAACCATGGATCTACCCTCCCAAGCAGAAACTTTCCACTACCTGGCAGCCAAAGAACAGG
  CGAGCACATAACTTGAAGGATTACATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225233 representing NM_001101560
  Red=Cloning site Green=Tags(s)
  
  MASAGLQLLAFILAVSGVSGVLAATLLPNWKVNAYAGPNIVTAVVQVQGLWVDCTWYSTGMFSCTLKYSI
  LSLPVHVQTARATMVLACVLSAWGICTSIAGMNCTHLGGDTHTKSKISFAGGVCFITAGISGLIPTVWYT
  KEIIANFLDLTIPESHKYEPGGALYIGFISAMLLLISGVIFCTSYIQKNQEPWIYPPKQKLSTTWQPKNR
  RAHNLKDYM
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001101560
• ORF Size: 657 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001101560.1, NP_001095030.1
• RefSeq Size: 660
• RefSeq ORF: 660
• Locus ID: 621628
• Gene Summary: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is identified in retinal pigment epithelium (RPE) and analysis of the RPE transcriptome reveals that this gene expression appears late during development of chick embryo. [provided by RefSeq, Aug 2010]