Mcoln1 (NM_053177) Mouse Tagged ORF Clone

CAT#: MG225390

Mcoln1 (GFP-tagged) - Mouse mucolipin 1 (Mcoln1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mcoln1
• Synonyms: 2210015I05Rik; mucolipidin; TRPML1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225390 representing NM_053177
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCCCGGCGGGCCGGCGCGCCTCAGAGACTGAGCGGCTGCTGACCCCCAATCCTGGGTATGGGA
  CCCAGGTGGGGACCTCACCAGCCCCAACAACCCCCACAGAAGAGGAAGACCTCCGCCGCCGCCTCAAGTA
  CTTCTTTATGAGCCCATGTGACAAGTTCCGGGCCAAAGGCCGCAAGCCCTGCAAGCTGATGCTGCAGGTG
  GTCAAGATCTTGGTGGTCACTGTGCAGCTCATTCTCTTTGGGCTCAGCAACCAGCTGGTGGTGACATTCC
  GGGAAGAGAACACCATTGCCTTCCGACATCTCTTCCTGCTGGGTTACTCTGATGGGTCTGATGACACCTT
  TGCAGCCTACACACAGGAGCAGCTCTACCAAGCCATCTTCTATGCTGTGGACCAGTACCTGATACTACCT
  GAGATATCCCTGGGCCGGTATGCCTATGTCCGTGGTGGGGGTGGGCCTTGGGCCAATGGATCAGCTTTGG
  CTCTCTGCCAGCGGTACTACCACCGTGGCCATGTGGACCCAGCCAATGATACCTTTGACATTGATCCAAG
  GGTAGTCACTGACTGTATCCAGGTGGATCCTCCTGACAGACCCCCTGACATCCCCAGTGAGGACTTGGAC
  TTCTTGGATGGCAGCGCCAGTTACAAGAACCTCACACTGAAATTCCACAAGCTGATCAACGTCACCATCC
  ACTTCCAGCTGAAGACAATTAACCTGCAGAGCCTCATCAACAATGAGATCCCTGATTGTTACACCTTCAG
  TATCCTGATCACATTTGACAATAAAGCGCACAGTGGGCGAATCCCCATCCGCCTGGAGACCAAGACCCAC
  ATCCAGGAGTGCAAACACCCCAGTGTCTCCAGACATGGAGACAACAGCTTCCGGCTTCTGTTTGATGTGG
  TGGTTATCCTCACCTGCTCCCTGTCCTTCCTGCTGTGCGCCCGCTCACTGCTCCGTGGCTTCCTGCTGCA
  GAACGAGTTTGTTGTATTCATGTGGCGGCGGCGGGGTCGGGAAATCAGCCTCTGGGAACGGCTGGAGTTT
  GTCAATGGCTGGTACATCCTGCTGGTCACCAGTGACGTGCTCACCATCTCGGGGACTGTCATGAAGATTG
  GCATTGAGGCAAAGAACCTAGCCAGCTATGATGTCTGCAGTATTCTCTTGGGTACCTCCACTCTGCTAGT
  CTGGGTCGGTGTCATTCGCTACCTGACATTTTTCCACAAGTACAACATCTTGATTGCCACGTTGCGAGTG
  GCACTGCCCAGTGTCATGCGTTTCTGCTGCTGTGTGGCTGTCATCTACCTGGGCTATTGCTTCTGTGGCT
  GGATCGTTCTAGGGCCCTACCATGTGAAGTTCCGCTCGCTGTCCATGGTTTCTGAGTGTCTGTTCTCACT
  CATCAACGGAGACGACATGTTTGTGACGTTCGCGGCCATGCAGGCCCAGCAGGGTCACAGCAGCCTGGTG
  TGGCTCTTCTCCCAGCTGTACCTCTACTCCTTCATCAGCCTCTTCATCTACATGGTGCTGAGCCTCTTCA
  TTGCACTCATCACCGGCGCCTATGACACCATCAAGCACCCAGGAGGTACTGGCACAGAGAAGAGTGAGCT
  CCAGGCCTACATCGAGCAGTGCCAGGATAGCCCCACATCTGGCAAGTTCCGTCGTGGCAGTGGCTCAGCT
  TGTAGCCTTTTCTGCTGCTGTGGAAGGGACTCCCCGGAGGACCATTCGCTGCTGGTGAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225390 representing NM_053177
  Red=Cloning site Green=Tags(s)
  
  MATPAGRRASETERLLTPNPGYGTQVGTSPAPTTPTEEEDLRRRLKYFFMSPCDKFRAKGRKPCKLMLQV
  VKILVVTVQLILFGLSNQLVVTFREENTIAFRHLFLLGYSDGSDDTFAAYTQEQLYQAIFYAVDQYLILP
  EISLGRYAYVRGGGGPWANGSALALCQRYYHRGHVDPANDTFDIDPRVVTDCIQVDPPDRPPDIPSEDLD
  FLDGSASYKNLTLKFHKLINVTIHFQLKTINLQSLINNEIPDCYTFSILITFDNKAHSGRIPIRLETKTH
  IQECKHPSVSRHGDNSFRLLFDVVVILTCSLSFLLCARSLLRGFLLQNEFVVFMWRRRGREISLWERLEF
  VNGWYILLVTSDVLTISGTVMKIGIEAKNLASYDVCSILLGTSTLLVWVGVIRYLTFFHKYNILIATLRV
  ALPSVMRFCCCVAVIYLGYCFCGWIVLGPYHVKFRSLSMVSECLFSLINGDDMFVTFAAMQAQQGHSSLV
  WLFSQLYLYSFISLFIYMVLSLFIALITGAYDTIKHPGGTGTEKSELQAYIEQCQDSPTSGKFRRGSGSA
  CSLFCCCGRDSPEDHSLLVN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053177
• ORF Size: 1740 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053177.1, NP_444407.1
• RefSeq Size: 2039
• RefSeq ORF: 1743
• Locus ID: 94178