Gabra2 (NM_008066) Mouse Tagged ORF Clone

CAT#: MG225561

Gabra2 (GFP-tagged) - Mouse gamma-aminobutyric acid (GABA) A receptor subunit alpha 2 (Gabra2), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gabra2
• Synonyms: C630048P16Rik; Gabra-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225561 representing NM_008066
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACAAAATTGAGCACATGCAATGTATGGTCTCTGCTGCTTGTTCTTCTGGTGTGGGACCCAGTCA
  GGTTGGTGCTGGCTAACATCCAAGAAGATGAGGCTAAAAATAACATCACCATCTTTACAAGAATTCTAGA
  CAGACTTCTGGATGGTTATGATAATCGGCTTAGACCAGGACTGGGAGACAGTATTACTGAAGTCTTCACT
  AACATTTACGTGACCAGTTTTGGCCCTGTCTCAGATACAGATATGGAGTATACAATAGATGTTTTCTTTC
  GGCAAAAATGGAAAGATGAGCGTTTAAAATTTAAAGGTCCTATGAATATCCTTCGACTTAACAATTTAAT
  GGCCAGCAAAATCTGGACTCCTGATACCTTCTTTCACAATGGGAAAAAGTCAGTGGCCCATAACATGACA
  ATGCCAAACAAGCTGCTTCGAATCCAGGATGATGGAACATTGCTATATACCATGAGGCTTACAGTCCAAG
  CCGAATGTCCCATGCACCTGGAGGATTTCCCGATGGATGCTCATTCATGCCCACTGAAATTTGGAAGCTA
  CGCTTACACAACCTCAGAAGTCACATATATTTGGACTTACAATGCTTCTGACTCCGTTCAGGTTGCTCCC
  GATGGCTCCAGGTTAAATCAGTATGATTTGCTGGGCCAGTCAATTGGGAAGGAAACAATTAAATCCAGTA
  CAGGTGAATATACTGTAATGACAGCTCATTTCCACTTGAAAAGAAAAATTGGGTATTTTGTGATTCAGAC
  CTATCTGCCTTGCATCATGACTGTCATTCTCTCCCAAGTGTCATTCTGGCTGAACAGAGAATCGGTGCCA
  GCAAGAACTGTGTTTGGAGTAACAACTGTTCTAACAATGACAACCTTGAGCATCAGTGCTCGAAATTCCC
  TCCCGAAAGTGGCTTATGCCACTGCCATGGACTGGTTTATAGCTGTTTGTTATGCGTTTGTGTTCTCTGC
  CCTAATTGAATTTGCAACCGTGAATTACTTCACAAAAAGAGGATGGGCTTGGGACGGGAAGAGTGTAGTC
  AATGACAAGAAAAAAGAGAAAGGCTCCGTCATGATACAGAACAACGCCTATGCGGTAGCTGTTGCCAACT
  ATGCCCCGAATCTTTCCAAAGATCCTGTCCTCTCTACCATTTCCAAAAGTGCAACGACGCCAGAACCGAA
  CAAGAAGCCAGAGAACAAGCCAGCTGAAGCCAAGAAAACCTTCAACAGTGTCAGCAAAATCGACAGGATG
  TCTAGAATAGTGTTCCCAGTTCTGTTTGGTACTTTTAATTTAGTTTATTGGGCTACCTATTTAAACAGGG
  AGCCTGTATTAGGGGTCAGTCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225561 representing NM_008066
  Red=Cloning site Green=Tags(s)
  
  MKTKLSTCNVWSLLLVLLVWDPVRLVLANIQEDEAKNNITIFTRILDRLLDGYDNRLRPGLGDSITEVFT
  NIYVTSFGPVSDTDMEYTIDVFFRQKWKDERLKFKGPMNILRLNNLMASKIWTPDTFFHNGKKSVAHNMT
  MPNKLLRIQDDGTLLYTMRLTVQAECPMHLEDFPMDAHSCPLKFGSYAYTTSEVTYIWTYNASDSVQVAP
  DGSRLNQYDLLGQSIGKETIKSSTGEYTVMTAHFHLKRKIGYFVIQTYLPCIMTVILSQVSFWLNRESVP
  ARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRGWAWDGKSVV
  NDKKKEKGSVMIQNNAYAVAVANYAPNLSKDPVLSTISKSATTPEPNKKPENKPAEAKKTFNSVSKIDRM
  SRIVFPVLFGTFNLVYWATYLNREPVLGVSP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008066
• ORF Size: 1353 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008066.3, NP_032092.1
• RefSeq Size: 2392
• RefSeq ORF: 1356
• Locus ID: 14395
• Gene Summary: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. [UniProtKB/Swiss-Prot Function]