Etv4 (NM_008815) Mouse Tagged ORF Clone

CAT#: MG225586

Etv4 (GFP-tagged) - Mouse ets variant gene 4 (E1A enhancer binding protein E1AF) (Etv4), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Etv4
• Synonyms: AW414408; Pea-3; Pea3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225586 representing NM_008815
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGAGGATGAAAGGCGGATACTTGGACCAGCGAGTGCCCTACACCTTCTGCAGCAAATCTCCCG
  GAAATGGGAGCTTGGGCGAAGCGCTGATGGTCCCGCAGGGAAAGCTCATGGACCCGGGCTCCCTGCCGCC
  TTCCGACTCAGAAGATCTCTTCCAGGACCTCAGTCACTTCCAAGAGACGTGGCTCGCAGAAGCTCAGGTA
  CCGGACAGTGATGAGCAGTTTGTTCCTGATTTCCATTCAGAAAACTCTTTCCATAGCCCCACCACCAGGA
  TCAAGAAGGAACCCCAGAGTCCCCGCACAGACCCCGCCCTGTCCTGCAGCAGGAAGCCACCACTCCCCTA
  CCACCATGGAGAGCAGTGCCTTTACTCCAGTGCCTATGACTCCCCCAGACAAATCGCCATCAAGTCCCCC
  GCCCCCGGTGCCCCTGGACAGTCGCCCCTGCAGCCCTTTTCCAGGGCAGAACAGCAGCAGAGCCTCCTGA
  GAGCCTCCAGCTCTTCCCAGTCCCACCCTGGCCACGGGTACCTTGGTGAGCACAGCTCCGTCTTCCAGCA
  GCCCGTGGACATGTGCCACTCCTTCACATCTCCTCAGGGAGGGGGCCGGGAACCTCTCCCAGCCCCCTAT
  CAACACCAACTGTCGGAGCCCTGCCCACCCTACCCCCAGCAGAACTTCAAGCAGGAGTACCATGACCCCC
  TGTACGAACAGGCTGGCCAGCCCGCTTCAAGCCAGGGTGGGGTCAGTGGGCACAGGTACCCAGGGGCGGG
  GGTGGTGATCAAACAGGAGCGCACAGACTTCGCCTACGACTCAGATGTCCCTGGATGTGCATCAATGTAC
  CTCCACCCAGAGGGCTTCTCTGGACCCTCTCCAGGTGATGGAGTGATGGGTTATGGCTATGAAAAATCCC
  TTCGACCATTCCCAGATGATGTCTGCATTGTCCCTGAAAAATTTGAAGGAGACATCAAGCAGGAAGGGAT
  TGGAGCTTTCCGGGAGGGGCCACCCTACCAGCGCCGGGGTGCCTTACAACTGTGGCAGTTTCTGGTGGCC
  CTGCTGGATGACCCAACAAATGCTCATTTCATTGCTTGGACAGGCCGGGGAATGGAGTTTAAACTAATTG
  AACCTGAAGAGGTTGCCAGGCTCTGGGGTATCCAGAAGAACCGGCCAGCCATGAATTATGACAAGCTGAG
  CCGCTCGCTGCGATACTATTATGAGAAAGGCATCATGCAGAAGGTGGCTGGCGAACGCTACGTGTACAAG
  TTTGTGTGCGAGCCGGAGGCCCTGTTCTCTCTGGCCTTCCCAGATAATCAACGTCCAGCTCTGAAGGCTG
  AGTTTGACCGGCCAGTCAGTGAGGAGGACACAGTCCCTTTGTCCCACTTGGATGAGAGTCCTGCCTACCT
  CCCAGAACTCACTGGCCCCGCTCCGCCCTTCGGCCACAGAGGTGGATATTCTTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225586 representing NM_008815
  Red=Cloning site Green=Tags(s)
  
  MERRMKGGYLDQRVPYTFCSKSPGNGSLGEALMVPQGKLMDPGSLPPSDSEDLFQDLSHFQETWLAEAQV
  PDSDEQFVPDFHSENSFHSPTTRIKKEPQSPRTDPALSCSRKPPLPYHHGEQCLYSSAYDSPRQIAIKSP
  APGAPGQSPLQPFSRAEQQQSLLRASSSSQSHPGHGYLGEHSSVFQQPVDMCHSFTSPQGGGREPLPAPY
  QHQLSEPCPPYPQQNFKQEYHDPLYEQAGQPASSQGGVSGHRYPGAGVVIKQERTDFAYDSDVPGCASMY
  LHPEGFSGPSPGDGVMGYGYEKSLRPFPDDVCIVPEKFEGDIKQEGIGAFREGPPYQRRGALQLWQFLVA
  LLDDPTNAHFIAWTGRGMEFKLIEPEEVARLWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYK
  FVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELTGPAPPFGHRGGYSY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008815
• ORF Size: 1455 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008815.2, NP_032841.2
• RefSeq Size: 2336
• RefSeq ORF: 1458
• Locus ID: 18612
• Gene Summary: Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'. May play a regulatory role during embryogenesis. [UniProtKB/Swiss-Prot Function]