Nid1 (NM_010917) Mouse Tagged ORF Clone

CAT#: MG225593

Nid1 (GFP-tagged) - Mouse nidogen 1 (Nid1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nid1
• Synonyms: A630025O17; entactin; entactin-1; Nid; nidogen-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225593 representing NM_010917
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGACGCGAGCGGCTGTAGTTGGGCGATGTGGACGTGGGCGCTGTTGCAGCTGCTGCTACTAGTGG
  GGCCCGGAGGCTGCCTGAATCGCCAGGAGCTCTTCCCCTTCGGCCCTGGGCAGGGGGACCTAGAGCTGGA
  GGCCGGGGACGACGTGGTATCCCCCTCCCTGGAACTGATCGGAGAACTGAGCTTCTATGATCGTACGGAC
  ATCACGTCGGTCTATGTCACCACAAATGGTATCATCGCCATGAGCGAACCCCCAGCCACAGAATACCATC
  CCGGAACCTTCCCACCCAGCTTCGGCTCAGTAGCGCCTTTCCTGGCTGACTTGGACACAACCGATGGCCT
  GGGGAATGTGTATTATCGAGAAGACTTATCCCCGTTCATTATTCAGATGGCAGCAGAGTATGTCCAGAGA
  GGCTTCCCGGAGGTCTCTTTCCAGCCCACTAGTGTGGTGGTTGTCACTTGGGAATCTGTGGCCCCTTATG
  GAGGGCCCAGCAGCAGCCCTGCCGAGGAAGGCAAGAGAAACACGTTCCAGGCTGTTCTGGCCTCCTCAAA
  TTCCAGCTCCTATGCCATTTTCCTTTATCCTGAAGATGGTCTACAGTTCTTTACAACATTCTCGAAGAAG
  GATGAAAGCCAAGTACCTGCTGTGGTTGGTTTCAGCAAAGGTCTAGTAGGATTTCTATGGAAGAGCAACG
  GAGCCTATAACATATTTGCCAATGACAGAGAATCAATTGAAAATTTGGCTAAGAGCAGCAACGCTGGGCA
  CCAGGGTGTGTGGGTGTTTGAGATTGGAAGTCCTGCTACCGCCAAGGGCGTGGTGTCTGCAGATGTGAAC
  CTGGACCTGGACGACGATGGAGCAGACTATGAAGATGAAGATTATGACCTAGTAACCTCTCACCTTGGCC
  TGGAGGATGTAGCCACTCCCTCCCCCTCCCACAGTCCAAGAAGGGGATACCCTGACCCACACAATGTACC
  TAGGATCCTCTCTCCTGGCTATGAGGCTACAGAGAGACCCCGTGGAGTCCCCACTGAGAGGACCAGATCT
  TTCCAGCTGCCAGCGGAGAGGTTCCCTCAGCATCACCCCCAGGTCATTGATGTGGATGAAGTAGAGGAAA
  CAGGAGTTGTATTCAGCTACAACACAGGTTCCCAGCAGACTTGTGCCAACAATAGACACCAGTGCTCCGT
  GCATGCAGAGTGCAGAGACTATGCTACTGGCTTCTGCTGCAGGTGTGTGGCCAACTACACAGGCAATGGC
  AGACAGTGCGTGGCAGAAGGCTCTCCACAACGGGTCAATGGCAAGGTGAAGGGAAGGATCTTCGTGGGGA
  GCAGCCAGGTCCCCGTGGTGTTTGAGAACACTGACCTGCACTCCTATGTGGTGATGAACCACGGGCGCTC
  TTACACAGCCATCAGCACCATCCCTGAAACCGTCGGCTACTCTCTGCTCCCCCTGGCACCCATTGGAGGC
  ATCATCGGATGGATGTTTGCAGTGGAGCAGGATGGGTTCAAGAATGGGTTTAGCATCACTGGGGGCGAGT
  TTACCCGGCAAGCTGAGGTGACCTTCCTGGGGCACCCAGGCAAGCTGGTCCTGAAGCAGCAGTTCAGCGG
  TATTGATGAACATGGACACCTGACCATCAGCACGGAGCTGGAGGGCCGCGTGCCGCAGATCCCCTATGGA
  GCCTCGGTGCACATTGAGCCCTACACCGAACTGTACCACTACTCCAGCTCAGTGATCACTTCCTCCTCCA
  CCCGGGAGTACACGGTGATGGAGCCTGATCAGGACGGCGCTGCACCCTCACACACCCATATTTACCAGTG
  GCGTCAGACCATCACCTTCCAGGAGTGTGCCCACGATGACGCCAGGCCAGCCCTGCCCAGCACCCAGCAG
  CTCTCTGTGGACAGCGTGTTTGTCCTGTACAACAAGGAGGAGAGGATCTTGCGCTATGCCCTCAGCAACT
  CCATCGGGCCTGTGAGGGATGGCTCCCCTGATGCCCTTCAGAATCCATGCTACATTGGCACCCATGGGTG
  TGACAGCAATGCTGCCTGTCGCCCTGGCCCTGGAACACAGTTCACCTGCGAATGCTCCATCGGCTTCCGA
  GGAGACGGGCAGACTTGCTATGATATTGATGAGTGTTCAGAGCAGCCTTCCCGCTGTGGGAACCATGCGG
  TCTGCAACAACCTCCCAGGAACCTTCCGCTGCGAGTGTGTAGAGGGCTACCACTTCTCAGACAGGGGAAC
  ATGTGTGGCTGCCGAGGACCAACGTCCCATCAACTACTGTGAAACTGGTCTCCACAACTGTGATATCCCC
  CAGCGAGCCCAGTGCATCTATATGGGTGGTTCCTCCTACACCTGCTCCTGTCTGCCTGGCTTCTCTGGGG
  ATGGCAGAGCCTGCCGAGACGTGGATGAATGCCAGCACAGCCGATGTCACCCCGATGCCTTCTGCTACAA
  CACACCAGGCTCTTTCACATGTCAGTGCAAGCCTGGCTATCAGGGGGATGGCTTCCGATGCATGCCCGGA
  GAGGTGAGCAAAACCCGGTGTCAACTGGAACGAGAGCACATCCTTGGAGCAGCCGGCGGGGCAGATGCAC
  AGCGGCCCACCCTGCAGGGGATGTTTGTACCTCAGTGTGATGAATATGGACACTATGTACCCACCCAGTG
  TCACCACAGCACTGGCTACTGCTGGTGTGTGGACCGAGATGGTCGGGAGCTGGAGGGTAGCCGTACCCCA
  CCTGGGATGAGGCCCCCGTGTCTGAGTACAGTGGCTCCTCCTATTCACCAGGGACCAGTAGTACCTACAG
  CTGTCATCCCCCTGCCTCCAGGGACACACTTACTCTTTGCTCAGACTGGAAAGATTGAACGCCTGCCCCT
  GGAGAGAAACACCATGAAGAAGACAGAAGCCAAGGCCTTTCTCCATATCCCTGCAAAAGTCATCATTGGA
  CTGGCCTTTGACTGCGTGGACAAGGTGGTTTACTGGACAGACATCAGCGAGCCTTCCATTGGGAGAGCCA
  GCCTCCACGGTGGAGAGCCAACCACCATCATTCGACAAGATCTTGGAAGCCCTGAAGGCATTGCCCTTGA
  CCATCTTGGTCGAACCATCTTCTGGACGGACTCTCAGTTGGATCGAATAGAAGTTGCAAAGATGGATGGC
  ACCCAGCGCCGAGTGCTGTTTGACACGGGTTTGGTGAATCCCAGAGGCATTGTGACAGACCCCGTAAGAG
  GGAACCTTTATTGGACAGATTGGAACAGAGACAATCCCAAAATTGAGACTTCTCACATGGATGGCACCAA
  CCGGAGGATTCTCGCACAGGACAACCTGGGCTTGCCCAATGGTCTGACCTTTGATGCATTCTCATCTCAG
  CTTTGCTGGGTGGATGCAGGCACCCATAGGGCAGAATGCCTGAACCCAGCTCAGCCCGGCAGACGCAAAG
  TTCTCGAAGGGCTCCAGTATCCTTTCGCTGTGACTAGCTATGGGAAGAATTTGTACTACACAGACTGGAA
  GACGAATTCAGTGATTGCCATGGACCTTGCTATATCCAAAGAGATGGATACCTTCCACCCACACAAGCAG
  ACCCGGCTATATGGCATCACCATCGCCCTGTCCCAGTGTCCCCAAGGCCACAATTACTGCTCAGTGAATA
  ATGGTGGATGTACCCACCTCTGCTTGCCCACTCCAGGGAGCAGGACCTGCCGATGTCCTGACAACACCCT
  GGGAGTTGACTGCATTGAACGGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225593 representing NM_010917
  Red=Cloning site Green=Tags(s)
  
  MLDASGCSWAMWTWALLQLLLLVGPGGCLNRQELFPFGPGQGDLELEAGDDVVSPSLELIGELSFYDRTD
  ITSVYVTTNGIIAMSEPPATEYHPGTFPPSFGSVAPFLADLDTTDGLGNVYYREDLSPFIIQMAAEYVQR
  GFPEVSFQPTSVVVVTWESVAPYGGPSSSPAEEGKRNTFQAVLASSNSSSYAIFLYPEDGLQFFTTFSKK
  DESQVPAVVGFSKGLVGFLWKSNGAYNIFANDRESIENLAKSSNAGHQGVWVFEIGSPATAKGVVSADVN
  LDLDDDGADYEDEDYDLVTSHLGLEDVATPSPSHSPRRGYPDPHNVPRILSPGYEATERPRGVPTERTRS
  FQLPAERFPQHHPQVIDVDEVEETGVVFSYNTGSQQTCANNRHQCSVHAECRDYATGFCCRCVANYTGNG
  RQCVAEGSPQRVNGKVKGRIFVGSSQVPVVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPIGG
  IIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFLGHPGKLVLKQQFSGIDEHGHLTISTELEGRVPQIPYG
  ASVHIEPYTELYHYSSSVITSSSTREYTVMEPDQDGAAPSHTHIYQWRQTITFQECAHDDARPALPSTQQ
  LSVDSVFVLYNKEERILRYALSNSIGPVRDGSPDALQNPCYIGTHGCDSNAACRPGPGTQFTCECSIGFR
  GDGQTCYDIDECSEQPSRCGNHAVCNNLPGTFRCECVEGYHFSDRGTCVAAEDQRPINYCETGLHNCDIP
  QRAQCIYMGGSSYTCSCLPGFSGDGRACRDVDECQHSRCHPDAFCYNTPGSFTCQCKPGYQGDGFRCMPG
  EVSKTRCQLEREHILGAAGGADAQRPTLQGMFVPQCDEYGHYVPTQCHHSTGYCWCVDRDGRELEGSRTP
  PGMRPPCLSTVAPPIHQGPVVPTAVIPLPPGTHLLFAQTGKIERLPLERNTMKKTEAKAFLHIPAKVIIG
  LAFDCVDKVVYWTDISEPSIGRASLHGGEPTTIIRQDLGSPEGIALDHLGRTIFWTDSQLDRIEVAKMDG
  TQRRVLFDTGLVNPRGIVTDPVRGNLYWTDWNRDNPKIETSHMDGTNRRILAQDNLGLPNGLTFDAFSSQ
  LCWVDAGTHRAECLNPAQPGRRKVLEGLQYPFAVTSYGKNLYYTDWKTNSVIAMDLAISKEMDTFHPHKQ
  TRLYGITIALSQCPQGHNYCSVNNGGCTHLCLPTPGSRTCRCPDNTLGVDCIERK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010917
• ORF Size: 3735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_010917.2, NP_035047.2
• RefSeq Size: 6046
• RefSeq ORF: 3738
• Locus ID: 18073
• Gene Summary: Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions. [UniProtKB/Swiss-Prot Function]