Pdgfrb (NM_008809) Mouse Tagged ORF Clone

CAT#: MG225618

Pdgfrb (GFP-tagged) - Mouse platelet derived growth factor receptor beta polypeptide (Pdgfrb) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pdgfrb
• Synonyms: AI528809; CD140b; Pdgfr; PDGFR-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225618 representing NM_008809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTTCCAGGAGTGATACCAGCTTTAGTCCTCAGAGGCCAGTTGTTGCTGTCCGTGTTATGGCTCC
  TGGGACCGCAGACCTCCCGGGGCCTAGTCATCACGCCCCCTGGGCCAGAGTTTGTTCTCAACATCTCCAG
  CACCTTTGTTCTGACCTGCTCGGGCTCAGCTCCGGTGATGTGGGAACAGATGTCCCAGGTGCCCTGGCAA
  GAAGCGGCCATGAATCAGGACGGCACCTTCTCCAGTGTGCTGACACTGACCAATGTCACTGGGGGAGACA
  CTGGGGAATACTTTTGTGTCTACAACAACTCACTAGGGCCGGAGCTCAGTGAGAGGAAGCGTATCTATAT
  CTTTGTGCCAGATCCCACCATGGACTTCCTCCCTATGGACTCCGAGGACCTGTTCATTTTTGTCACGGAT
  GTCACTGAGACGACAATTCCGTGCCGAGTGACAGACCCCCAGCTGGAGGTGACGCTACATGAGAAGAAAG
  TGGATATCCCCCTACACGTACCCTACGACCACCAGCGAGGTTTCACTGGTACTTTTGAGGACAAGACTTA
  CATTTGCAAAACCACCATTGGGGACAGGGAAGTGGACTCCGATACTTACTACGTCTACAGCCTCCAGGTG
  TCATCCATCAACGTCTCTGTGAATGCCGTGCAGACTGTGGTCCGCCAGGGCGAGAGCATCACCATCCGGT
  GCATTGTGATGGGCAATGATGTGGTGAACTTCCAATGGACGTACCCCCGCATGAAGAGTGGGCGGCTGGT
  GGAGCCAGTGACAGACTACCTCTTTGGAGTGCCCTCCCGCATTGGCTCCATCCTGCATATCCCCACGGCT
  GAGCTGAGTGATTCGGGCACCTATACTTGCAACGTGTCAGTGAGTGTGAACGACCATGGCGATGAGAAAG
  CCATCAACATCTCTGTGATCGAGAATGGCTACGTGCGGCTGCTGGAGACACTGGGAGATGTAGAAATTGC
  TGAGCTGCACCGGAGTCGGACGCTGCGGGTGGTGTTCGAGGCTTATCCGATGCCTTCTGTCCTGTGGCTC
  AAGGACAACCGTACCTTGGGTGACTCCGGCGCTGGCGAGTTAGTTTTGTCTACTCGCAACATGTCTGAGA
  CCCGGTACGTGTCAGAACTGATCCTGGTACGTGTGAAGGTGTCAGAAGCGGGCTACTATACTATGCGAGC
  CTTCCACGAGGACGATGAGGTCCAGCTCTCCTTCAAGCTGCAGGTCAATGTCCCCGTCCGTGTGCTGGAG
  CTGAGTGAGAGTCACCCTGCCAATGGGGAGCAGACAATCCGCTGTCGTGGCCGGGGCATGCCTCAGCCAA
  ATGTCACCTGGTCTACCTGCAGAGACCTCAAAAGTAGGTGTCCACGAAAACTGTCACCCACACCCTTGGG
  GAATAGTTCCAAGGAGGAGAGCCAGCTAGAAACGAATGTGACTTTCTGGGAGGAAGATCAGGAATACGAG
  GTGGTGAGCACACTGCGCCTGCGCCACGTGGATCAGCCACTGTCCGTACGCTGCATGCTGCAGAACTCCA
  TGGGTGGAGATTCGCAGGAGGTCACCGTGGTCCCACATTCCTTGCCCTTCAAAGTGGTGGTGATCTCAGC
  CATCCTGGCCTTAGTGGTCCTTACCGTCATCTCTCTCATCATCCTCATCATGCTGTGGCAGAAGAAGCCA
  CGCTATGAGATCCGATGGAAGGTCATTGAGTCTGTGAGCTCTGACGGTCATGAGTACATCTACGTGGACC
  CTGTGCAGTTGCCTTACGACTCCACCTGGGAGCTGCCACGGGACCAGCTTGTTCTGGGACGCACTCTTGG
  CTCTGGGGCTTTCGGACAGGTGGTGGAGGCCACAGCTCACGGTCTGAGCCATTCGCAGGCCACCATGAAA
  GTGGCTGTCAAGATGCTGAAATCGACAGCCAGAAGTAGCGAGAAGCAAGCCTTAATGTCCGAGCTGAAGA
  TTATGAGTCATCTTGGACCCCACCTGAACGTGGTCAACCTGCTGGGGGCCTGCACCAAAGGAGGGCCCAT
  CTACATCATCACGGAATACTGCCGATACGGTGATCTGGTGGACTACCTGCACCGGAACAAACACACCTTC
  TTGCAGCGACACTCCAACAAGCATTGTCCGCCCAGTGCTGAGCTCTACAGCAACGCCCTGCCAGTGGGGT
  TCTCCCTACCCAGCCACTTGAACCTGACTGGGGAGAGTGACGGTGGCTACATGGATATGAGCAAGGATGA
  ATCTATAGATTACGTGCCCATGTTGGACATGAAAGGAGACATCAAATACGCAGACATTGAGTCCCCCAGC
  TACATGGCCCCTTATGATAACTATGTCCCATCTGCCCCTGAAAGGACCTATCGCGCCACCTTAATCAACG
  ACTCACCAGTGCTCAGCTACACAGACCTCGTGGGCTTCAGCTACCAAGTGGCCAACGGCATGGACTTCTT
  AGCCTCTAAGAACTGTGTTCACCGAGACTTGGCGGCCAGGAATGTGCTCATCTGCGAGGGCAAGCTGGTC
  AAGATCTGTGACTTCGGCCTGGCTCGAGACATCATGAGGGACTCAAACTACATCTCCAAAGGCAGCACCT
  TCCTGCCTCTGAAGTGGATGGCCCCAGAGAGCATCTTCAACAGCCTCTACACCACTTTGAGTGATGTCTG
  GTCTTTTGGGATCCTACTCTGGGAGATCTTCACACTGGGTGGCACCCCTTACCCAGAGCTGCCCATGAAC
  GACCAGTTCTACAATGCCATCAAGAGGGGCTACCGCATGGCCCAGCCTGCTCATGCCTCCGACGAGATCT
  ATGAGATCATGCAGAAATGCTGGGAAGAAAAGTTTGAGACTCGACCCCCCTTCTCCCAGCTGGTGCTGCT
  CCTGGAGAGGCTTCTGGGTGAAGGCTATAAAAAGAAGTACCAGCAGGTAGATGAGGAGTTCCTGAGGAGT
  GACCATCCTGCCATCCTGAGGTCCCAAGCCCGCTTTCCGGGGATCCACAGCCTCCGATCCCCTCTGGACA
  CCAGCTCTGTTCTCTACACTGCCGTGCAGCCCAATGAGAGTGACAATGACTACATCATCCCCTTACCTGA
  CCCCAAGCCTGACGTTGCTGATGAAGGTCTCCCAGAGGGGTCCCCCAGCCTTGCCAGTTCCACCTTGAAT
  GAAGTCAACACTTCCTCCACCATCTCCTGCGACAGTCCCCTGGAGCTCCAAGAAGAGCCACAGCAAGCAG
  AGCCTGAGGCACAACTGGAGCAGCCACAGGATTCAGGCTGCCCAGGACCTCTGGCTGAAGCAGAGGACAG
  CTTCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225618 representing NM_008809
  Red=Cloning site Green=Tags(s)
  
  MGLPGVIPALVLRGQLLLSVLWLLGPQTSRGLVITPPGPEFVLNISSTFVLTCSGSAPVMWEQMSQVPWQ
  EAAMNQDGTFSSVLTLTNVTGGDTGEYFCVYNNSLGPELSERKRIYIFVPDPTMDFLPMDSEDLFIFVTD
  VTETTIPCRVTDPQLEVTLHEKKVDIPLHVPYDHQRGFTGTFEDKTYICKTTIGDREVDSDTYYVYSLQV
  SSINVSVNAVQTVVRQGESITIRCIVMGNDVVNFQWTYPRMKSGRLVEPVTDYLFGVPSRIGSILHIPTA
  ELSDSGTYTCNVSVSVNDHGDEKAINISVIENGYVRLLETLGDVEIAELHRSRTLRVVFEAYPMPSVLWL
  KDNRTLGDSGAGELVLSTRNMSETRYVSELILVRVKVSEAGYYTMRAFHEDDEVQLSFKLQVNVPVRVLE
  LSESHPANGEQTIRCRGRGMPQPNVTWSTCRDLKSRCPRKLSPTPLGNSSKEESQLETNVTFWEEDQEYE
  VVSTLRLRHVDQPLSVRCMLQNSMGGDSQEVTVVPHSLPFKVVVISAILALVVLTVISLIILIMLWQKKP
  RYEIRWKVIESVSSDGHEYIYVDPVQLPYDSTWELPRDQLVLGRTLGSGAFGQVVEATAHGLSHSQATMK
  VAVKMLKSTARSSEKQALMSELKIMSHLGPHLNVVNLLGACTKGGPIYIITEYCRYGDLVDYLHRNKHTF
  LQRHSNKHCPPSAELYSNALPVGFSLPSHLNLTGESDGGYMDMSKDESIDYVPMLDMKGDIKYADIESPS
  YMAPYDNYVPSAPERTYRATLINDSPVLSYTDLVGFSYQVANGMDFLASKNCVHRDLAARNVLICEGKLV
  KICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMN
  DQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFETRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRS
  DHPAILRSQARFPGIHSLRSPLDTSSVLYTAVQPNESDNDYIIPLPDPKPDVADEGLPEGSPSLASSTLN
  EVNTSSTISCDSPLELQEEPQQAEPEAQLEQPQDSGCPGPLAEAEDSFL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008809
• ORF Size: 3294 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008809.2, NP_032835.2
• RefSeq Size: 5410
• RefSeq ORF: 3297
• Locus ID: 18596