Gclm (NM_008129) Mouse Tagged ORF Clone

CAT#: MG225622

Gclm (GFP-tagged) - Mouse glutamate-cysteine ligase modifier subunit (Gclm), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gclm
• Synonyms: AI649393; Gcmc; Glclr
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225622 representing NM_008129
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCGACAGCCGCGCGGCCGGGGCGCTCCTGGCGCGGGCCAGCACCCTGCACCTGCAGACCGGGA
  ACCTGCTCAACTGGGGCCGCCTGCGAAAAAAGTGCCCGTCCACGCACAGCGAGGAGCTTCGGGACTGTAT
  CCAAAAGACCTTGAATGAATGGAGTTCCCAAATCAGCCCCGATTTAGTCAGGGAGTTTCCAGATGTTTTG
  GAATGCACCATGTCCCATGCAGTGGAGAAGATAAATCCCGATGAAAGAGAAGAAATGAAAGTTTCTGCAA
  AACTGTTCATTGTAGGATCGAATTCTTCATCATCAACTAGAAGTGCAGTTGACATGGCATGCTCCGTCCT
  TGGAGTTGCACAGCTGGACTCTGTGATCATGGCTTCGCCTCCGATTGAAGATGGAGTTAATCTTTCCTTG
  GAGCATTTACAGCCTTACTGGGAGGAATTAGAGAACTTAGTTCAGAGCAAGAAAATTGTTGCTATAGGCA
  CCTCTGATCTAGACAAGACACAGTTGGAGCAGCTGTATCAGTGGGCACAGGTAAAACCCAATAGTAACCA
  AGTTAACCTGGCCTCCTGCTGTGTGATGCCACCAGATTTGACTGCCTTTGCTAAACAATTTGACATACAG
  CTACTGACTCACAATGACCCGAAAGAACTGCTCTCTGAGGCAAGTTTCCAGGAAGCTCTTCAAGAAAGCA
  TCCCTGACATTGAAGCCCAGGATTGGGTGCCGCTGTGGCTGCTGAGGTACTCGGTCATCGTGAAGAGCAG
  GGGAATCATCAAGTCTAAAGGCTACATTCTGCAAGCCAAAAGGAGGGGCTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225622 representing NM_008129
  Red=Cloning site Green=Tags(s)
  
  MGTDSRAAGALLARASTLHLQTGNLLNWGRLRKKCPSTHSEELRDCIQKTLNEWSSQISPDLVREFPDVL
  ECTMSHAVEKINPDEREEMKVSAKLFIVGSNSSSSTRSAVDMACSVLGVAQLDSVIMASPPIEDGVNLSL
  EHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQ
  LLTHNDPKELLSEASFQEALQESIPDIEAQDWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008129
• ORF Size: 822 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008129.3, NP_032155.1
• RefSeq Size: 1470
• RefSeq ORF: 825
• Locus ID: 14630