Cldn22 (NM_029383) Mouse Tagged ORF Clone

CAT#: MG225640

Cldn22 (GFP-tagged) - Mouse claudin 22 (Cldn22), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cldn22
• Synonyms: 2210404A22Rik; 5330431C14Rik; 9530051B05Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225640 representing NM_029383
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTAGTCTTCCGAACGGCAACGCAGGCGGCTGCACTTCTGCTCTCCCTGCTGGGATGGGTTTTAT
  CCTGCCTTACAAACTACTTGCCGCACTGGAAGAACCTTAACTTGGAGCTGAACGAGATGGAGAACTGGAC
  CATGGGGCTCTGGAAATCCTGCGTCATCCAGGAGGAAGTCGGGAGGCAATGCAAGGACTTTGACTCCTTC
  CTGGCCTTGCCAGCTGAACTGCAGGTCTCCAGGGTCTTGATGTCCCTGTGCAACGGGCTGGGGCTGCTGG
  GCTTGCTGGCCTCTGGCTGTGGCCTGGACTGCTTGAGGCTTGGAGAGACACAGGAGGGTCTGAAGAAGCG
  GCTGCTCACCCTGGGAGGGACCCTGCTCTGGACCTCGGGAGTCATGGTGCTAGTTCCTGTCTCCTGGGTG
  GCCCACAAGACGGTGCGGGAGTTCTGGGATGAGACCATGCCTGAGATTGTGCCCAGGTGGGAGTTTGGGG
  AGGCCCTGTTCCTGGGCTGGTTTGCTGGCTTCTGCCTGGTGCTAGGAGGGTGTGTGCTTCACTGTGCAGC
  CTGCTGGAGCCCGGCTCCTGCAGCCTCGAGTCACTATGCAGTGGCGGGACCTCGAGACCACCAGCAGCAT
  CTGGAGCTGAAGCAAGCCAATCCAGAAATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225640 representing NM_029383
  Red=Cloning site Green=Tags(s)
  
  MGLVFRTATQAAALLLSLLGWVLSCLTNYLPHWKNLNLELNEMENWTMGLWKSCVIQEEVGRQCKDFDSF
  LALPAELQVSRVLMSLCNGLGLLGLLASGCGLDCLRLGETQEGLKKRLLTLGGTLLWTSGVMVLVPVSWV
  AHKTVREFWDETMPEIVPRWEFGEALFLGWFAGFCLVLGGCVLHCAACWSPAPAASSHYAVAGPRDHQQH
  LELKQANPEI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029383
• ORF Size: 660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_029383.1, NP_083659.1
• RefSeq Size: 994
• RefSeq ORF: 663
• Locus ID: 75677
• Gene Summary: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is intronless and overlaps the 3' UTR of the Wwc2 gene (GeneID: 52357) on the opposite strand. [provided by RefSeq, Aug 2010]