Grin1 (NM_008169) Mouse Tagged ORF Clone

CAT#: MG225704

Grin1 (GFP-tagged) - Mouse glutamate receptor ionotropic NMDA1 (zeta 1) (Grin1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grin1
• Synonyms: GluN1; GluRdelta1; GluRzeta1; M100174; NMD-R1; Nmdar; NMDAR1; NR1; Rgsc174
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225704 representing NM_008169
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACCATGCACCTGCTGACATTCGCCCTGCTTTTCTCCTGCTCCTTCGCCCGCGCTGCCTGCGACC
  CCAAGATTGTCAACATCGGCGCGGTGCTGAGCACGCGCAAGCACGAGCAGATGTTCCGCGAGGCAGTAAA
  CCAGGCCAATAAGCGACACGGCTCTTGGAAGATACAGCTCAACGCCACTTCTGTCACCCACAAGCCCAAC
  GCCATACAGATGGCCCTGTCAGTGTGTGAGGACCTCATCTCTAGCCAGGTCTACGCTATCCTAGTTAGTC
  ACCCGCCTACTCCCAACGACCACTTCACTCCCACCCCTGTCTCCTACACAGCTGGCTTCTACAGAATCCC
  CGTCCTGGGGCTGACTACCCGAATGTCCATCTACTCTGACAAGAGCATCCACCTGAGCTTCCTTCGCACC
  GTACCACCCTACTCCCACCAGTCCAGCGTCTGGTTTGAGATGATGCGCGTCTACAACTGGAACCATATCA
  TCCTGCTGGTCAGCGATGACCACGAGGGCCGGGCAGCGCAGAAGCGCCTGGAGACGTTGCTGGAGGAGCG
  TGAGTCCAAGGCAGAGAAGGTGCTGCAGTTTGACCCAGGAACCAAGAATGTGACGGCTCTGCTGATGGAA
  GCCCGGGACCTGGAAGCCCGGGTCATCATCCTTTCTGCAAGCGAGGACGACGCTGCCACCGTATACCGCG
  CAGCCGCGATGCTGAACATGACTGGCTCTGGGTACGTGTGGCTCGTCGGGGAGCGCGAGATCTCTGGGAA
  TGCCCTGCGCTACGCTCCTGACGGCATCATCGGACTTCAGCTAATCAACGGCAAGAACGAGTCGGCCCAC
  ATCAGTGACGCTGTGGGCGTGGTGGCACAGGCAGTCCACGAGCTCCTAGAAAAGGAGAACATCACTGATC
  CACCGCGGGGTTGCGTGGGCAACACCAACATCTGGAAGACAGGACCACTGTTCAAGAGGGTGCTGATGTC
  TTCCAAGTATGCAGATGGAGTGACTGGCCGTGTGGAATTCAATGAGGATGGGGACCGGAAGTTTGCCAAC
  TATAGTATCATGAACCTGCAGAACCGCAAGCTGGTGCAAGTGGGCATCTACAATGGTACCCATGTCATCC
  CAAATGACAGGAAGATCATCTGGCCAGGAGGAGAGACAGAGAAGCCTCGAGGATACCAGATGTCCACCAG
  ACTAAAGATAGTGACAATCCACCAAGAACCCTTCGTGTATGTCAAGCCCACAATGAGTGATGGCACATGC
  AAAGAGGAGTTCACAGTCAATGGTGACCCTGTCAAGAAGGTGATCTGTACGGGGCCTAATGACACATCCC
  CAGGAAGCCCACGTCACACAGTGCCCCAGTGCTGTTATGGCTTCTGCGTTGACCTGCTCATCAAGCTGGC
  ACGGACCATGAATTTTACCTACGAGGTGCACCTTGTGGCAGATGGCAAGTTTGGCACACAGGAGCGGGTA
  AACAACAGCAACAAAAAGGAGTGGAACGGAATGATGGGAGAGCTGCTCAGTGGTCAAGCAGACATGATCG
  TGGCTCCACTGACCATTAACAATGAGCGTGCGCAGTACATAGAGTTCTCCAAGCCCTTCAAGTACCAGGG
  CCTGACCATTCTGGTCAAGAAGGAGATCCCTCGGAGCACACTGGACTCATTCATGCAGCCCTTTCAGAGC
  ACACTGTGGCTGCTGGTGGGGCTGTCAGTTCATGTGGTGGCCGTGATGCTGTACCTGCTGGACCGCTTCA
  GTCCCTTTGGCCGATTTAAGGTGAACAGCGAGGAGGAGGAGGAGGATGCACTGACCCTGTCCTCTGCCAT
  GTGGTTTTCCTGGGGCGTCCTGCTCAACTCTGGCATTGGGGAAGGTGCCCCCCGGAGTTTCTCTGCTCGT
  ATCCTAGGCATGGTGTGGGCTGGTTTTGCCATGATCATCGTGGCTTCCTACACTGCCAACCTGGCAGCCT
  TCCTGGTGCTGGATAGGCCTGAGGAGCGCATCACAGGCATCAATGACCCCAGGCTCAGAAACCCCTCAGA
  CAAGTTCATCTATGCAACTGTAAAACAGAGCTCTGTGGATATCTACTTCCGGAGGCAGGTGGAGTTGAGC
  ACCATGTACCGGCACATGGAGAAGCACAATTATGAGAGTGCAGCTGAGGCCATCCAGGCTGTGCGGGACA
  ACAAGCTCCATGCCTTCATCTGGGACTCAGCTGTGCTGGAGTTTGAGGCTTCACAGAAGTGCGATCTGGT
  GACCACGGGTGAGCTGTTCTTCCGCTCCGGCTTTGGCATCGGCATGCGCAAGGACAGCCCCTGGAAGCAA
  AATGTGTCCCTGTCCATACTCAAGTCCCATGAGAATGGCTTCATGGAAGACCTGGATAAGACATGGGTTC
  GGTATCAAGAATGTGACTCCCGCAGCAATGCCCCTGCCACCCTCACTTTTGAGAACATGGCAGGGGTCTT
  CATGCTGGTGGCTGGAGGCATCGTAGCTGGGATCTTCCTCATTTTCATCGAGATCGCCTACAAGCGACAC
  AAGGATGCCCGTAGGAAGCAGATGCAGCTGGCTTTTGCAGCCGTGAACGTGTGGAGGAAGAACCTGCAGG
  ATAGAAAGAGTGGTAGAGCAGAGCCCGACCCTAAAAAGAAAGCCACATTTAGGGCTATCACCTCCACCCT
  GGCCTCCAGCTTCAAGAGACGTAGGTCCTCCAAAGACACGAGCACCGGGGGTGGACGCGGCGCTTTGCAA
  AACCAAAAAGACACAGTGCTGCCGCGACGCGCTATTGAGAGGGAGGAGGGCCAGCTGCAGCTGTGTTCCC
  GTCATAGGGAGAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225704 representing NM_008169
  Red=Cloning site Green=Tags(s)
  
  MSTMHLLTFALLFSCSFARAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPN
  AIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRT
  VPPYSHQSSVWFEMMRVYNWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLME
  ARDLEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGIIGLQLINGKNESAH
  ISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFAN
  YSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTMSDGTC
  KEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCVDLLIKLARTMNFTYEVHLVADGKFGTQERV
  NNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQS
  TLWLLVGLSVHVVAVMLYLLDRFSPFGRFKVNSEEEEEDALTLSSAMWFSWGVLLNSGIGEGAPRSFSAR
  ILGMVWAGFAMIIVASYTANLAAFLVLDRPEERITGINDPRLRNPSDKFIYATVKQSSVDIYFRRQVELS
  TMYRHMEKHNYESAAEAIQAVRDNKLHAFIWDSAVLEFEASQKCDLVTTGELFFRSGFGIGMRKDSPWKQ
  NVSLSILKSHENGFMEDLDKTWVRYQECDSRSNAPATLTFENMAGVFMLVAGGIVAGIFLIFIEIAYKRH
  KDARRKQMQLAFAAVNVWRKNLQDRKSGRAEPDPKKKATFRAITSTLASSFKRRRSSKDTSTGGGRGALQ
  NQKDTVLPRRAIEREEGQLQLCSRHRES
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008169
• ORF Size: 2814 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008169.2, NP_032195.1
• RefSeq Size: 3215
• RefSeq ORF: 2817
• Locus ID: 14810