Sgk1 (NM_001161845) Mouse Tagged ORF Clone

CAT#: MG225721

Sgk1 (GFP-tagged) - Mouse serum/glucocorticoid regulated kinase 1 (Sgk1) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sgk1
• Synonyms: Sgk
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225721 representing NM_001161845
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGGATTCCCGGTCAAGAAATGCTCAGCGTTCCAATTTTTTAAGAAACGGGTACGAAGATGGATCA
  AGAGCCCCATGGTCAGCGTGGACAAGCATCAGAGCCCCAACTTGAAGTACACTGGCCCTGCTGGGGTGCA
  TCTTCCCCCTGGGGAGTCAGACTTTGAGGCCATGTGTCAATCATGCCTGGGTGACCATGCTTTCCAAAGG
  GGGATGCTCCCTCCAGAGGAGTCCTGTTCCTGGGAGATCCAACCTGGGTGTGAAGTGAAAGAACAATGTA
  ATCATGCCAACATCCTGACCAAGCCGGACCCAAGAACCTTCTGGACTAATGATGATGCAGCTTTTATGAA
  ACAGAGAAGGATGGGCCTGAACGATTTTATTCAGAAGATTGCCAGCAACACCTATGCATGCAAACACGCT
  GAAGTTCAGTCCATTTTGAAAATGTCCCATCCTCAGGAGCCGGAGCTTATGAACGCTAACCCCTCTCCTC
  CGCCAAGTCCCTCTCAACAAATCAACCTGGGTCCGTCCTCCAACCCTCACGCCAAACCCTCCGACTTTCA
  CTTCTTGAAAGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTGGCTAGGCACAAGGCAGAAGAAGTA
  TTCTATGCAGTCAAAGTTTTACAGAAGAAAGCCATCCTGAAGAAGAAAGAGGAGAAGCATATTATGTCAG
  AGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTTCTCATTCCAGACCGC
  TGACAAGCTCTACTTTGTCCTGGACTACATTAATGGTGGAGAGCTGTTCTACCATCTCCAGAGGGAGCGC
  TGCTTCCTGGAACCACGGGCTCGATTCTACGCAGCTGAAATAGCCAGTGCCCTGGGCTATCTGCACTCCC
  TAAACATCGTTTATAGAGACTTAAAACCTGAGAATATTCTCCTAGACTCCCAGGGGCACATCGTCCTCAC
  TGACTTTGGGCTCTGCAAAGAGAATATTGAGCATAACGGGACAACATCTACCTTCTGTGGCACGCCTGAG
  TATCTGGCTCCTGAGGTCCTCCATAAGCAGCCGTATGACCGGACGGTGGACTGGTGGTGTCTTGGGGCTG
  TCCTGTATGAGATGCTCTACGGCCTGCCCCCGTTTTATAGCCGGAACACGGCTGAGATGTACGACAATAT
  TCTGAACAAGCCTCTCCAGTTGAAACCAAATATTACAAACTCGGCAAGGCACCTCCTGGAAGGCCTCCTG
  CAGAAGGACCGGACCAAGAGGCTGGGTGCCAAGGATGACTTTATGGAGATTAAGAGTCATATTTTCTTCT
  CTTTAATTAACTGGGATGATCTCATCAATAAGAAGATTACACCCCCATTTAACCCAAATGTGAGTGGGCC
  CAGTGACCTTCGGCACTTCGATCCCGAGTTTACCGAGGAGCCGGTCCCCAGCTCCATCGGCAGGTCCCCT
  GACAGCATCCTTGTCACGGCCAGTGTGAAGGAAGCAGCAGAAGCCTTCCTCGGCTTCTCCTATGCACCTC
  CTGTGGATTCCTTCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225721 representing NM_001161845
  Red=Cloning site Green=Tags(s)
  
  MNGFPVKKCSAFQFFKKRVRRWIKSPMVSVDKHQSPNLKYTGPAGVHLPPGESDFEAMCQSCLGDHAFQR
  GMLPPEESCSWEIQPGCEVKEQCNHANILTKPDPRTFWTNDDAAFMKQRRMGLNDFIQKIASNTYACKHA
  EVQSILKMSHPQEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEV
  FYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRER
  CFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNGTTSTFCGTPE
  YLAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLL
  QKDRTKRLGAKDDFMEIKSHIFFSLINWDDLINKKITPPFNPNVSGPSDLRHFDPEFTEEPVPSSIGRSP
  DSILVTASVKEAAEAFLGFSYAPPVDSFL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161845
• ORF Size: 1572 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001161845.2, NP_001155317.2
• RefSeq Size: 3121
• RefSeq ORF: 1575
• Locus ID: 20393
• Gene Summary: This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. This enzyme is activated by protein phosphorylation and degraded via the ubiquitination and proteasome pathway. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene was identified on chromosome 12. [provided by RefSeq, Sep 2009]