Chrna5 (NM_176844) Mouse Tagged ORF Clone

CAT#: MG225729

Chrna5 (GFP-tagged) - Mouse cholinergic receptor nicotinic alpha polypeptide 5 (Chrna5), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chrna5
• Synonyms: Acra-5; Acra5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225729 representing NM_176844
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGATCTCGAATGCAGGGTTGCCTGAGCTATCCTCTGCTGCAAAACATGAAGACAGTTTGTTTAGGG
  ATTTATTTGAAGACTATGAAAAGTGGGTTCGTCCTGTGGAACACCTGAGTGACAAGATAAAAATCAAGTT
  TGGCCTTGCAATATCTCAGTTAGTGGACGTGGATGAGAAAAACCAGCTAATGACCACCAACGTCTGGTTG
  AAGCAGGAATGGATAGATGTAAAGTTAAGATGGAATCCTGATGACTATGGTGGAATAAAAATTATACGTG
  TTCCTTCAGACTCTCTGTGGATCCCAGACATCGTTTTGTTTGATAATGCAGACGGACGTTTCGAAGGGGC
  CAGTACGAAAACAGTTGTCAGGTACAATGGCACTGTCACTTGGACGCAGCCAGCAAACTACAAAAGTTCT
  TGCACTATAGATGTGACCTTTTTCCCATTTGATCTCCAAAATTGCTCCATGAAATTCGGCTCATGGACGT
  ATGATGGATCCCAGGTCGATATAATCCTAGAGGACCAAGATGTCGACAGAACAGACTTTTTTGACAATGG
  AGAATGGGAAATCATGAGCGCAATGGGGAGCAAGGGGAACCGGACGGACAGCTGCTGCTGGTACCCCTGC
  ATCACCTACTCCTTCGTGATCAAACGGCTGCCTCTCTTCTACACCCTGTTTCTTATCATACCCTGCATCG
  GGCTCTCGTTTCTGACTGTGGTTGTCTTCTATCTCCCTTCAAACGAAGGTGAAAAGATTAGCCTGTGCAC
  TTCCGTGCTGGTCTCTCTGACTGTCTTCCTGCTGGTGATTGAGGAAATTATACCGTCATCTTCCAAAGTC
  ATACCTCTGATCGGGGAGTACTTGGTGTTCACCATGATCTTCGTGACCCTATCCATTATGGTGACTGTCT
  TTGCCATCAACATCCACCACCGCTCTTCTTCCACACACAATGCTATGGCGCCGTGGGTTCGTAAGATATT
  TCTCCACAAGCTTCCCAAACTGCTCTGCATGAGAAGTCATGCCGATAGGTACTTCACTCAGAGAGAAGAA
  GCCGAGAAAGACGGTGGACCTAAATCTCGGAATACTTTGGAGGCCGCGCTCGATTGCATTCGCTACATCA
  CGAGACACGTAGTGAAAGAGAACGACGTCCGCGAGGTTGTTGAAGATTGGAAATTCATAGCCCAAGTTCT
  CGATCGGATGTTTCTATGGACGTTTCTTCTGGTTTCAATCATTGGGACGCTAGGGCTTTTTGTTCCTGTT
  ATTTATAAATGGGCCAATATAATAGTCCCAGTTCACATTGGAAACACAATTAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225729 representing NM_176844
  Red=Cloning site Green=Tags(s)
  
  MQISNAGLPELSSAAKHEDSLFRDLFEDYEKWVRPVEHLSDKIKIKFGLAISQLVDVDEKNQLMTTNVWL
  KQEWIDVKLRWNPDDYGGIKIIRVPSDSLWIPDIVLFDNADGRFEGASTKTVVRYNGTVTWTQPANYKSS
  CTIDVTFFPFDLQNCSMKFGSWTYDGSQVDIILEDQDVDRTDFFDNGEWEIMSAMGSKGNRTDSCCWYPC
  ITYSFVIKRLPLFYTLFLIIPCIGLSFLTVVVFYLPSNEGEKISLCTSVLVSLTVFLLVIEEIIPSSSKV
  IPLIGEYLVFTMIFVTLSIMVTVFAINIHHRSSSTHNAMAPWVRKIFLHKLPKLLCMRSHADRYFTQREE
  AEKDGGPKSRNTLEAALDCIRYITRHVVKENDVREVVEDWKFIAQVLDRMFLWTFLLVSIIGTLGLFVPV
  IYKWANIIVPVHIGNTIK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176844
• ORF Size: 1314 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_176844.4, NP_789814.2
• RefSeq Size: 2738
• RefSeq ORF: 1317
• Locus ID: 110835