Cry1 (NM_007771) Mouse Tagged ORF Clone

CAT#: MG225732

Cry1 (GFP-tagged) - Mouse cryptochrome 1 (photolyase-like) (Cry1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cry1
• Synonyms: AU020726; AU021000; Phll1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225732 representing NM_007771
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTGAACGCCGTGCACTGGTTCCGAAAGGGACTCCGGCTCCACGACAACCCCGCCCTGAAGGAGT
  GCATCCAGGGCGCCGACACCATCCGCTGCGTCTATATCCTCGACCCCTGGTTCGCCGGCTCTTCCAACGT
  GGGCATCAACAGGTGGCGATTTTTGCTTCAGTGTCTTGAGGATCTTGATGCCAATCTACGAAAATTAAAT
  TCTCGTCTGTTTGTGATTCGGGGACAGCCAGCTGATGTATTTCCCAGGCTTTTCAAGGAATGGAACATCA
  CTAAACTCTCAATTGAGTATGATTCTGAGCCTTTTGGGAAGGAACGAGATGCAGCTATCAAGAAGCTGGC
  TACTGAGGCTGGCGTGGAAGTCATCGTGCGCATTTCACATACACTGTATGACCTGGACAAGATCATAGAA
  CTCAATGGCGGACAGCCACCTCTAACATATAAAAGGTTTCAGACTCTCGTCAGCAAGATGGAGCCACTGG
  AGATGCCAGCAGACACCATCACATCAGATGTGATAGGAAAGTGCATGACCCCTCTGTCTGATGACCATGA
  TGAGAAATATGGCGTTCCTTCCCTGGAAGAGCTCGGCTTTGATACAGATGGCCTGTCCTCTGCAGTGTGG
  CCAGGAGGAGAAACTGAGGCACTTACACGTTTGGAAAGGCATTTGGAAAGAAAGGCCTGGGTGGCAAACT
  TTGAACGACCTCGAATGAATGCAAACTCCCTGCTTGCAAGCCCAACTGGACTCAGTCCTTATCTCCGCTT
  TGGTTGTTTATCATGTCGGCTGTTTTATTTCAAACTAACAGATCTCTACAAAAAGGTAAAGAAGAATAGT
  TCCCCTCCCCTTTCTCTTTATGGGCAACTCCTGTGGCGTGAATTTTTTTATACAGCAGCCACAAACAACC
  CACGCTTTGACAAAATGGAAGGGAACCCCATCTGTGTTCAGATCCCTTGGGACAAGAACCCCGAGGCTCT
  GGCCAAATGGGCAGAAGGCCGGACAGGCTTCCCGTGGATTGACGCCATCATGACTCAGCTTCGTCAGGAG
  GGCTGGATCCACCATTTAGCCAGACACGCGGTTGCCTGTTTCCTGACTCGTGGTGACCTGTGGATCAGCT
  GGGAAGAAGGGATGAAGGTCTTTGAAGAGTTACTGCTTGATGCAGATTGGAGCATAAATGCTGGAAGTTG
  GATGTGGCTGTCCTGCAGTTCCTTTTTTCAGCAATTTTTTCACTGCTACTGCCCTGTGGGTTTTGGTAGG
  AGGACAGATCCCAATGGAGACTATATTAGGCGTTATTTACCTGTCCTAAGAGGCTTCCCTGCAAAATATA
  TCTACGATCCTTGGAATGCACCAGAAGGCATCCAGAAGGTTGCCAAGTGTTTGATAGGAGTTAATTACCC
  CAAACCGATGGTGAACCATGCTGAGGCAAGCAGACTGAATATTGAAAGAATGAAGCAGATCTATCAGCAG
  CTTTCCCGGTACAGAGGGCTAGGTCTTCTCGCCTCGGTCCCTTCTAACTCTAATGGGAATGGAGGGCTCA
  TGGGCTATGCTCCTGGAGAGAATGTCCCGAGTTGTAGCAGCAGCGGGAATGGAGGGCTCATGGGCTATGC
  TCCTGGAGAGAACGTCCCGAGCTGTAGCGGTGGAAATTGCTCTCAAGGAAGTGGTATTTTACACTATGCT
  CACGGGGACAGTCAGCAGACTCACTCACTCAAGCAAGGGAGAAGCTCCGCGGGCACCGGCCTCAGCAGTG
  GGAAGCGTCCTAGTCAGGAAGAGGATGCCCAGAGTGTCGGCCCCAAAGTCCAGCGGCAGAGCAGTAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225732 representing NM_007771
  Red=Cloning site Green=Tags(s)
  
  MGVNAVHWFRKGLRLHDNPALKECIQGADTIRCVYILDPWFAGSSNVGINRWRFLLQCLEDLDANLRKLN
  SRLFVIRGQPADVFPRLFKEWNITKLSIEYDSEPFGKERDAAIKKLATEAGVEVIVRISHTLYDLDKIIE
  LNGGQPPLTYKRFQTLVSKMEPLEMPADTITSDVIGKCMTPLSDDHDEKYGVPSLEELGFDTDGLSSAVW
  PGGETEALTRLERHLERKAWVANFERPRMNANSLLASPTGLSPYLRFGCLSCRLFYFKLTDLYKKVKKNS
  SPPLSLYGQLLWREFFYTAATNNPRFDKMEGNPICVQIPWDKNPEALAKWAEGRTGFPWIDAIMTQLRQE
  GWIHHLARHAVACFLTRGDLWISWEEGMKVFEELLLDADWSINAGSWMWLSCSSFFQQFFHCYCPVGFGR
  RTDPNGDYIRRYLPVLRGFPAKYIYDPWNAPEGIQKVAKCLIGVNYPKPMVNHAEASRLNIERMKQIYQQ
  LSRYRGLGLLASVPSNSNGNGGLMGYAPGENVPSCSSSGNGGLMGYAPGENVPSCSGGNCSQGSGILHYA
  HGDSQQTHSLKQGRSSAGTGLSSGKRPSQEEDAQSVGPKVQRQSSN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007771
• ORF Size: 1818 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007771.3, NP_031797.1
• RefSeq Size: 3035
• RefSeq ORF: 1821
• Locus ID: 12952
• Gene Summary: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of this gene results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Feb 2014]