Polr2a (NM_009089) Mouse Tagged ORF Clone
CAT#: MG225735
- TrueORF®
Polr2a (GFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide A (Polr2a), (10ug). Note: ORF is codon optimized
"NM_009089" in other vectors (2)
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Other products for "Polr2a"
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Polr2a |
Synonyms | 220kDa; Rpb1; Rpo2-1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG225735 representing NM_009089, codon optimized.
Due to the complexity of NM_009089, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACGGGGGTGGCCCCCCCTCCGGGGACAGCGCATGCCCGCTGCGCACCATCAAGAGAGTGCAGTTCG GAGTCCTGAGTCCGGATGAATTGAAGCGGATGTCTGTGACAGAGGGTGGTATCAAATACCCAGAAACAAC GGAGGGAGGTCGCCCCAAACTTGGGGGACTAATGGATCCACGGCAGGGGGTGATTGAGCGGACTGGCCGC TGCCAAACATGTGCAGGAAACATGACCGAGTGTCCTGGCCACTTTGGCCACATCGAATTGGCCAAACCTG TGTTTCATGTGGGCTTCCTGGTGAAGACAATGAAGGTTTTGCGCTGTGTCTGCTTCTTCTGCTCCAAACT GCTTGTAGATTCTAATAACCCGAAGATTAAGGACATCCTGGCCAAATCTAAGGGGCAGCCAAAGAAACGG CTTACGCATGTCTATGACCTGTGCAAGGGCAAAAACATCTGTGAAGGTGGAGAGGAGATGGACAACAAGT TTGGTGTGGAGCAGCCGGAGGGTGATGAGGATCTGACCAAAGAGAAGGGCCATGGCGGCTGTGGGCGGTA CCAACCCCGAATCCGGCGCTCTGGCCTAGAGTTGTATGCTGAATGGAAGCATGTTAATGAGGACTCTCAG GAGAAAAAGATCTTGCTGAGTCCAGAACGAGTGCATGAAATCTTCAAACGCATCTCAGATGAGGAGTGTT TTGTGTTGGGCATGGAGCCTCGCTATGCCCGGCCTGAGTGGATGATTGTCACAGTGTTGCCTGTGCCTCC TCTCTCAGTGAGACCTGCTGTGGTGATGCAGGGTTCTGCTCGAAACCAGGATGATCTGACTCACAAACTG GCTGACATTGTGAAGATCAACAATCAGCTGCGGCGGAACGAGCAGAATGGTGCAGCTGCCCATGTCATAG CAGAAGATGTAAAACTTCTCCAGTTCCATGTGGCCACCATGGTGGACAATGAACTGCCCGGCTTACCCCG TGCCATGCAGAAGTCTGGCCGTCCCCTCAAGTCCCTGAAGCAACGGTTGAAGGGCAAAGAAGGACGGGTT CGAGGGAATCTAATGGGCAAGCGAGTGGACTTCTCAGCCCGCACTGTCATCACCCCTGACCCTAACCTAT CCATTGACCAAGTGGGTGTGCCCCGCTCCATTGCTGCCAACATGACCTTTGCAGAGATTGTCACACCCTT CAATATTGACAGACTTCAGGAATTAGTACGCAGGGGAAACAGCCAGTATCCAGGAGCCAAGTACATCATC CGCGATAATGGCGATCGAATTGACTTGCGTTTCCATCCTAAGCCCAGTGACCTTCATCTGCAGACTGGCT ATAAGGTGGAACGGCACATGTGTGATGGGGACATTGTTATCTTCAATCGTCAGCCAACTTTGCACAAAAT GTCCATGATGGGGCATCGGGTCCGAATCCTCCCCTGGTCTACTTTTCGGTTGAATCTTAGTGTGACAACT CCATACAATGCAGACTTTGATGGAGATGAGATGAATTTGCATCTGCCACAGTCTCTGGAAACACGGGCAG AGATCCAAGAGTTGGCTATGGTGCCTCGAATGATTGTCACCCCCCAGAGCAATCGACCTGTCATGGGTAT TGTGCAAGACACACTCACGGCAGTACGCAAATTCACTAAGAGAGATGTCTTCCTGGAGCGGGGTGAAGTC ATGAACCTCTTAATGTTTCTGTCCACGTGGGATGGCAAGGTCCCACAACCAGCTATCCTCAAGCCCCGGC CCCTGTGGACAGGCAAGCAGATCTTCTCCCTCATCATACCTGGACACATCAATTGTATCCGTACCCACAG CACCCATCCTGATGATGAAGATAGTGGCCCTTACAAGCACATCTCTCCTGGGGACACCAAAGTTGTAGTG GAGAATGGGGAGCTTATCATGGGCATCCTTTGTAAGAAGTCCCTGGGCACCTCAGCAGGCTCGCTGGTCC ATATCTCCTACCTGGAGATGGGGCATGACATCACCCGCCTCTTCTACTCCAACATTCAGACTGTCATTAA CAACTGGCTTCTCATCGAGGGTCATACCATTGGCATTGGGGACTCCATTGCAGATTCAAAGACTTACCAG GACATTCAGAACACTATTAAGAAAGCTAAACAAGATGTAATAGAGGTCATTGAGAAGGCTCATAACAATG AGCTAGAACCCACTCCAGGAAACACATTGCGTCAGACATTTGAGAATCAAGTGAATCGTATTCTCAATGA TGCTCGAGACAAAACTGGCTCCTCTGCACAGAAATCCCTCTCTGAATATAACAACTTCAAGTCTATGGTG GTGTCTGGAGCCAAGGGTTCCAAGATCAACATCTCCCAGGTCATTGCTGTTGTCGGGCAGCAGAATGTAG AGGGCAAGCGGATCCCATTTGGATTCAAGCATCGGACTCTTCCTCACTTTATCAAGGATGATTATGGTCC TGAGAGCCGAGGCTTTGTAGAAAATTCATACTTGGCTGGTCTCACACCCACTGAGTTCTTCTTCCATGCC ATGGGAGGACGAGAAGGTCTCATTGACACGGCTGTTAAGACTGCTGAGACTGGGTATATTCAGCGAAGGC TGATAAAGTCCATGGAGTCGGTGATGGTGAAATATGATGCAACTGTGAGGAACTCCATCAACCAGGTGGT ACAGCTGCGCTATGGAGAGGACGGCCTGGCAGGGGAGAGTGTTGAGTTCCAGAATCTGGCTACACTTAAA CCTTCTAATAAAGCTTTTGAGAAGAAGTTCCGTTTTGATTATACCAATGAGAGGGCCCTGCGGCGCACCC TTCAGGAGGACCTGGTAAAGGATGTGCTGAGCAATGCCCATATACAGAATGAGCTGGAACGGGAATTTGA ACGGATGCGTGAAGATCGGGAAGTGCTCAGGGTCATCTTCCCAACTGGTGACAGCAAGGTGGTCCTCCCT TGTAACCTTCTGCGGATGATCTGGAATGCTCAGAAAATCTTCCACATCAACCCTCGCCTTCCCTCTGACC TGCATCCCATCAAAGTGGTAGAGGGAGTCAAAGAATTGAGCAAGAAGTTGGTAATTGTGAATGGAGATGA CCCACTAAGTCGGCAGGCACAGGAGAATGCCACATTGCTCTTCAACATCCACCTAAGATCCACACTCTGC TCTCGCCGCATGGCTGAGGAGTTTCGGCTGAGTGGAGAGGCCTTTGACTGGCTGCTTGGGGAGATAGAGT CCAAGTTCAACCAAGCCATTGCCCATCCTGGAGAAATGGTGGGAGCTCTGGCTGCTCAGTCTCTTGGAGA ACCTGCCACTCAGATGACCCTGAACACTTTCCACTATGCTGGTGTATCTGCCAAGAATGTGACGCTGGGT GTGCCTCGACTTAAGGAGCTTATCAACATTTCCAAGAAGCCAAAGACTCCTTCACTCACTGTCTTCCTGT TAGGTCAATCTGCACGAGATGCTGAAAGAGCCAAGGACATTCTTTGCCGCTTGGAACATACAACATTGAG GAAGGTGACTGCCAATACAGCCATCTACTATGACCCTAACCCCCAGAGCACAGTGGTGGCAGAAGATCAG GAGTGGGTGAATGTGTACTATGAGATGCCCGACTTTGACGTGGCCCGGATATCTCCCTGGCTGCTGCGTG TGGAGCTCGACCGAAAGCACATGACTGACCGGAAGCTTACCATGGAACAGATTGCTGAGAAAATCAATGC AGGTTTTGGCGATGATTTGAATTGTATCTTCAACGATGATAATGCAGAGAAGCTGGTCCTTCGAATCCGC ATCATGAACAGTGATGAAAACAAGATGCAAGAGGAGGAAGAGGTGGTGGATAAAATGGATGATGATGTCT TCCTGCGATGCATTGAGTCCAACATGCTGACAGATATGACCCTGCAGGGTATCGAGCAGATCAGCAAGGT GTACATGCACTTACCTCAGACAGACAACAAGAAGAAGATCATCATCACAGAGGATGGGGAGTTCAAGGCC CTGCAGGAATGGATCTTAGAGACGGATGGTGTGAGCCTGATGCGGGTGCTGAGTGAGAAGGATGTAGACC CTGTGCGCACCACGTCCAATGATATTGTGGAGATCTTCACGGTACTGGGCATTGAGGCTGTGCGGAAGGC TCTGGAGCGAGAACTGTACCATGTCATCTCCTTTGATGGTTCTTATGTCAATTATCGGCATTTGGCGCTC CTGTGTGACACTATGACATGCCGTGGCCACTTGATGGCCATCACTCGTCATGGGGTCAACCGCCAGGACA CTGGACCGCTCATGAAATGCTCCTTTGAGGAAACGGTGGATGTCCTTATGGAAGCAGCAGCACATGGGGA GAGTGACCCGATGAAGGGAGTCTCTGAGAATATTATGCTGGGCCAGCTCGCTCCAGCTGGTACTGGCTGT TTTGACCTCCTGCTTGATGCTGAGAAGTGCAAATACGGCATGGAAATCCCCACCAATATCCCTGGCCTGG GGGCTGCTGGACGGAGCGGGATGACCCCAGGAGCGGCCGGCTTCTCTCCCAGTGCTGCATCTGATGCCAG TGGCTTCAGCCCAGGTTACTCCCCTGCATGGTCTCCCACACCAGGCTCTCCGGGCTCCCCTGGACCCTCA AGCCCATACATCCCCTCACCAGGTGGTGCTATGTCTCCCAGCTACTCACCGACATCACCAGCCTATGAGC CACGCTCCCCTGGGGGCTATACACCCCAGAGCCCCTCCTACTCCCCTACTTCACCTTCCTACTCCCCAAC CTCTCCATCTTACTCTCCAACCAGTCCCAACTACAGCCCTACCTCTCCTAGCTACTCGCCCACCTCCCCT AGCTACTCGCCAACCTCTCCTTCCTACTCCCCCACCTCTCCAAGCTATTCCCCAACCTCTCCTAGCTACT CCCCAACCTCTCCAAGCTATTCTCCAACATCACCTAGCTATTCTCCAACTTCTCCCAGCTACTCACCGAC ATCTCCTAGCTACTCCCCAACTTCTCCCAGCTACTCACCAACTTCACCAAGCTATTCTCCCACCTCCCCC AGCTACTCACCGACATCTCCCAGCTACTCACCAACTTCTCCAAGCTACTCACCAACTTCTCCAAGCTACT CACCCACCAGCCCTAACTATTCTCCAACTAGTCCCAACTATACCCCGACATCACCCAGCTACAGCCCAAC CTCACCCAGTTACTCACCTACAAGTCCCAACTATACACCCACCAGCCCTAATTACAGCCCAACCTCTCCA AGCTATTCCCCAACCTCACCCAGTTATTCCCCCACCTCACCAAGCTACTCCCCCTCCAGCCCACGATATA CACCACAGTCTCCAACCTACACACCAAGCTCACCAAGCTACAGTCCCAGCTCACCAAGCTACAGCCCCAC TTCACCCAAGTACACCCCAACTAGTCCTTCCTACAGTCCCAGCTCACCAGAGTACACCCCAGCTTCTCCC AAATACTCACCTACAAGCCCTAAATATTCACCCACTTCTCCCAAGTATTCTCCTACCAGCCCCACTTACT CACCTACCACCCCAAAATATTCTCCAACCTCCCCGACATACTCACCAACCTCTCCAGTCTATACCCCGAC CTCTCCCAAGTACTCCCCAACCAGCCCTACCTACTCGCCCACTTCTCCCAAGTACTCGCCCACCAGTCCC ACCTACTCACCCACCTCTCCCAAGGGCTCCACCTACTCTCCCACTTCTCCTGGCTACTCACCCACTAGCC CCACCTACAGCCTCACCAGCCCAGCCATCAGCCCAGATGACAGCGATGAGGAGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225735 representing NM_009089
Red=Cloning site Green=Tags(s) MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGR CQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKR LTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQ EKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKL ADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRV RGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYII RDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTT PYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEV MNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVV ENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQ DIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMV VSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHA MGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLK PSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLP CNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLC SRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLG VPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQ EWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIR IMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKA LQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLAL LCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGC FDLLLDAEKCKYGMEIPTNIPGLGAAGRSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPS SPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSP SYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSP SYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSP SYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPTSPKYTPTSPSYSPSSPEYTPASP KYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSP TYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN TRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009089 |
ORF Size | 5796 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Reference Data | |
RefSeq | NM_009089.2, NP_033115.1 |
RefSeq Size | 5799 |
RefSeq ORF | 5799 |
Locus ID | 20020 |
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