Sgk1 (NM_001161847) Mouse Tagged ORF Clone

CAT#: MG225796

Sgk1 (GFP-tagged) - Mouse serum/glucocorticoid regulated kinase 1 (Sgk1) transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sgk1
• Synonyms: Sgk
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225796 representing NM_001161847
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACAGAGAAGGATGGGCCTGAACGATTTTATTCAGAAGATTGCCAGCAACACCTATGCATGCAAAC
  ACGCTGAAGTTCAGTCCATTTTGAAAATGTCCCATCCTCAGGAGCCGGAGCTTATGAACGCTAACCCCTC
  TCCTCCGCCAAGTCCCTCTCAACAAATCAACCTGGGTCCGTCCTCCAACCCTCACGCCAAACCCTCCGAC
  TTTCACTTCTTGAAAGTGATCGGAAAGGGCAGTTTTGGAAAGGTTCTTCTGGCTAGGCACAAGGCAGAAG
  AAGTATTCTATGCAGTCAAAGTTTTACAGAAGAAAGCCATCCTGAAGAAGAAAGAGGAGAAGCATATTAT
  GTCAGAGCGGAATGTTCTGTTGAAGAATGTGAAGCACCCTTTCCTGGTGGGCCTTCACTTCTCATTCCAG
  ACCGCTGACAAGCTCTACTTTGTCCTGGACTACATTAATGGTGGAGAGCTGTTCTACCATCTCCAGAGGG
  AGCGCTGCTTCCTGGAACCACGGGCTCGATTCTACGCAGCTGAAATAGCCAGTGCCCTGGGCTATCTGCA
  CTCCCTAAACATCGTTTATAGAGACTTAAAACCTGAGAATATTCTCCTAGACTCCCAGGGGCACATCGTC
  CTCACTGACTTTGGGCTCTGCAAAGAGAATATTGAGCATAACGGGACAACATCTACCTTCTGTGGCACGC
  CTGAGTATCTGGCTCCTGAGGTCCTCCATAAGCAGCCGTATGACCGGACGGTGGACTGGTGGTGTCTTGG
  GGCTGTCCTGTATGAGATGCTCTACGGCCTGCCCCCGTTTTATAGCCGGAACACGGCTGAGATGTACGAC
  AATATTCTGAACAAGCCTCTCCAGTTGAAACCAAATATTACAAACTCGGCAAGGCACCTCCTGGAAGGCC
  TCCTGCAGAAGGACCGGACCAAGAGGCTGGGTGCCAAGGATGACTTTATGGAGATTAAGAGTCATATTTT
  CTTCTCTTTAATTAACTGGGATGATCTCATCAATAAGAAGATTACACCCCCATTTAACCCAAATGTGAGT
  GGGCCCAGTGACCTTCGGCACTTCGATCCCGAGTTTACCGAGGAGCCGGTCCCCAGCTCCATCGGCAGGT
  CCCCTGACAGCATCCTTGTCACGGCCAGTGTGAAGGAAGCAGCAGAAGCCTTCCTCGGCTTCTCCTATGC
  ACCTCCTGTGGATTCCTTCCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225796 representing NM_001161847
  Red=Cloning site Green=Tags(s)
  
  MKQRRMGLNDFIQKIASNTYACKHAEVQSILKMSHPQEPELMNANPSPPPSPSQQINLGPSSNPHAKPSD
  FHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQ
  TADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIV
  LTDFGLCKENIEHNGTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYD
  NILNKPLQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIKSHIFFSLINWDDLINKKITPPFNPNVS
  GPSDLRHFDPEFTEEPVPSSIGRSPDSILVTASVKEAAEAFLGFSYAPPVDSFL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161847
• ORF Size: 1212 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001161847.2, NP_001155319.1
• RefSeq Size: 2622
• RefSeq ORF: 1215
• Locus ID: 20393
• Gene Summary: This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. This enzyme is activated by protein phosphorylation and degraded via the ubiquitination and proteasome pathway. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene was identified on chromosome 12. [provided by RefSeq, Sep 2009]