Mylk3 (NM_175441) Mouse Tagged ORF Clone

CAT#: MG225821

Mylk3 (GFP-tagged) - Mouse myosin light chain kinase 3 (Mylk3), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mylk3
• Synonyms: D830007F02Rik; MLCK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225821 representing NM_175441
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGAGTTTCAGAGGAGGACCCAGAGGGTCTGGCCCCCCAGGGTCTGCCAGCATTGGGCGGAGCCT
  GCTTAGCCACCATGGACAAAAAACTTAATGTGCTGACTGAGAAGGTCGACAGACTCTTGCATTTCCAAGA
  AGATGTCACAGAGAAGCTGCAGTGTGTGTGCCAAGGCATGGATCACCTGGAACAAGATCTGCATCGGCTG
  GAGGCCTCCCGGGAGTTGAGTCTGGCAGGGTCTGGCAGCACTCCCCCAACCACGGCTCAGGCCGCATGGC
  CTGAGGTCCTGGAGCTGGTGAGGGCTGTGCGGCAGGAGGGTGCCCAGCATGGTGCCAGGCTCGAAGCCCT
  CTTCAAGATGGTGGTGGCTGTGGACAGGGCTATTACTTTGGTAGGGTCCACATTCCAGAATTCCAAGGTG
  GCTGATTTCATCATGCAAGGAACCGTGCCCGGGAGAAAAGGCAGTCTGGCCGATGGCCCGGAGGAGAACA
  AGGAGCAAGCAGAAGTTGCTGGAGTGAAGCCAAACCATGTACTGACTACAGGAGGTGTGCAAGCTGACGC
  CTCTAGGACGCTGTGGGAAGAGAGCCAAAAGGAGGACATACCTGTGCGAACAGTGGAGGGGCTGCCTCTC
  ATCATCAATACGTCACTGAAGGGAGCTGACCTAACCCAGGCAGGAGCCTCACTGAGGCAGGGAGTTGAAG
  TTCTTGGCCCAGGCCAAGTACCCCTACCCACAGAAGCAGAATCCAGGCTTCCTGAGACAGCCAGTGAGAA
  CACTGGAGCCACCCTGGAATTGTCCGTAGCAATCGACAGAATCAGCGAGGTCCTCACTAGCCTCAAGATG
  TCACAAGGTGGTGGTCAAGAAACCTCATCCAGCAAGCCTGACTGTTGGCTTTCAGAAGAGGCCATGAGGC
  TGAGTTCAGGGCCTCTCCCTCAGCCCCTAGGACCACTAACTCCAGACAGTGACATTCACAGTGGTGATGC
  ACTTCCCAGGATCCCTATCAATATGCAAGAGATGGCTACTCCTGGGGAGTTGCTTGAGACCCAAAGTGGC
  AGTCCCATTGGCTCTGCAGAAGCTCCAGGCCTTGGGACTGTGTTAGAAGACCAGATCCCTAAAGGAGCCA
  GACCATTTCCACCCCTGCCAAAGAGGAGCAGCAACAATGGTGGCATGAGTGCAGAGGAGGAGATAGGGTC
  TGGGGCTGAGCCTATGAGAGGACCAAGCTTGGCTACAAGGGACTGGAGAGATGAGACTGTTGGGACCACA
  GACCTGCAGCAAGGCATAGACCCAGGAGCAGTGAGCCCTGAGCCTGGGAAGGACCACGCAGCCCAGGGCC
  CAGGAAGAACTGAAGCTGGAAGGCTATCTTCTGCTGCAGAGGCTGCCATTGTGGTTCTAGATGACAGCGC
  AGCACCCCCAGCCCCTTTTGAACACCGGGTAGTGAGCATCAAAGATACCCTGATCTCAGCAGGCTACACG
  GTATCCCAACATGAAGTCTTAGGAGGGGGTCGGTTTGGCCAGGTGCACAGGTGTACAGAGAGGTCTACAG
  GCCTTGCACTGGCAGCCAAGATCATCAAAGTGAAGAACGTAAAGGACCGGGAGGATGTGAAGAATGAGGT
  CAACATCATGAACCAGCTCAGCCACGTAAACTTGATCCAACTTTATGATGCGTTTGAGAGCAAGAGCAGC
  TTCACTCTGATCATGGAGTATGTGGATGGAGGCGAACTCTTTGACCGGATCACGGATGAGAAGTACCACC
  TCACTGAGTTGGATGTGGTCTTGTTCACGAGGCAGATCTGTGAGGGTGTGCATTACCTGCATCAGCACTA
  TATCCTGCACCTGGACCTCAAGCCTGAGAACATATTGTGTGTCAGCCAGACAGGGCATCAAATTAAGATC
  ATTGACTTTGGGCTGGCTAGAAGATACAAGCCTCGGGAGAAGCTAAAGGTGAACTTTGGTACTCCGGAGT
  TCCTGGCCCCAGAAGTTGTTAACTATGAGTTTGTGTCATTTCCAACAGACATGTGGAGTGTGGGAGTTAT
  CACCTACATGCTACTCAGTGGTTTGTCCCCATTTCTAGGGGAGACAGATGCAGAGACCATGAATTTTATT
  GTGAACTGCAGCTGGGATTTCGATGCTGATACCTTCAAAGGGCTGTCGGAGGAAGCCAAGGACTTTGTTT
  CCCGGTTACTGGTCAAAGAGAAGAGCTGTAGGATGAGCGCCACACAGTGCCTGAAACACGAGTGGTTAAG
  TCACCTGCCTGCCAAAGCCTCGGGCTCCAACGTTCGCCTCAGATCCCAACAACTGCTGCAGAAATATATG
  GCTCAGAGTAAATGGAAGAAACATTTCCACGTGGTGACTGCAGTCAACAGGCTACGGAAATTTCCAACGT
  GTCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225821 representing NM_175441
  Red=Cloning site Green=Tags(s)
  
  MSGVSEEDPEGLAPQGLPALGGACLATMDKKLNVLTEKVDRLLHFQEDVTEKLQCVCQGMDHLEQDLHRL
  EASRELSLAGSGSTPPTTAQAAWPEVLELVRAVRQEGAQHGARLEALFKMVVAVDRAITLVGSTFQNSKV
  ADFIMQGTVPGRKGSLADGPEENKEQAEVAGVKPNHVLTTGGVQADASRTLWEESQKEDIPVRTVEGLPL
  IINTSLKGADLTQAGASLRQGVEVLGPGQVPLPTEAESRLPETASENTGATLELSVAIDRISEVLTSLKM
  SQGGGQETSSSKPDCWLSEEAMRLSSGPLPQPLGPLTPDSDIHSGDALPRIPINMQEMATPGELLETQSG
  SPIGSAEAPGLGTVLEDQIPKGARPFPPLPKRSSNNGGMSAEEEIGSGAEPMRGPSLATRDWRDETVGTT
  DLQQGIDPGAVSPEPGKDHAAQGPGRTEAGRLSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDTLISAGYT
  VSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNVKDREDVKNEVNIMNQLSHVNLIQLYDAFESKSS
  FTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQTGHQIKI
  IDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETDAETMNFI
  VNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLSHLPAKASGSNVRLRSQQLLQKYM
  AQSKWKKHFHVVTAVNRLRKFPTCP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175441
• ORF Size: 2385 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_175441.5, NP_780650.2
• RefSeq Size: 3070
• RefSeq ORF: 2388
• Locus ID: 213435