Map2k3 (NM_008928) Mouse Tagged ORF Clone

CAT#: MG225898

Map2k3 (GFP-tagged) - Mouse mitogen-activated protein kinase kinase 3 (Map2k3), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Map2k3
• Synonyms: AW212142; MAPKK 3; MEK3; MKK3; MKK3b; Prkmk3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225898 representing NM_008928
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCGCCCGCCGCGAGCCCGCCGGCCAGCTTGCCTCAGACCAAAGGAAAATCCAAAAGGAAAAAGG
  ACTTACGGATATCCTGCGTGTCCAAGCCACCTGTGTCCAACCCCACACCCCCCCGGAACCTGGACTCCCG
  GACCTTCATCACTATCGGAGACAGAAACTTCGAAGTGGAGGCTGATGACTTGGTGACCATCTCAGAGCTG
  GGTCGTGGAGCCTATGGGGTGGTAGAGAAAGTGCGGCATGCTCAGAGTGGTACCATCATGGCTGTCAAGC
  GCATCCGGGCCACAGTGAACACACAGGAGCAGAAACGTCTGCTTATGGACCTAGACATCAACATGCGCAC
  GGTCGACTGCTTCTACACTGTCACCTTCTATGGTGCCCTCTTCAGAGAGGGGGATGTATGGATCTGCATG
  GAGCTCATGGACACTTCCCTGGATAAGTTCTACCGGAAGGTGCTGGAGAAGAACATGAAAATTCCGGAAG
  ACATCCTGGGGGAGATCGCTGTGTCTATCGTGCGGGCCCTGGAGCACCTGCATAGCAAGCTGTCTGTGAT
  CCACAGAGATGTGAAGCCATCCAATGTCCTCATCAACAAGGAAGGGCATGTGAAGATGTGCGACTTTGGC
  ATCAGTGGCTACCTGGTGGACTCTGTGGCAAAGACAATGGATGCTGGCTGCAAGCCTTACATGGCCCCTG
  AGAGGATCAACCCTGAACTGAATCAGAAGGGCTACAATGTCAAGTCTGATGTCTGGAGCCTCGGCATCAC
  CATGATCGAGATGGCCATTCTGCGATTCCCTTATGAGTCTTGGGGCACACCGTTCCAGCAGCTGAAGCAG
  GTGGTGGAGGAGCCATCCCCACAGCTCCCAGCGGACCAGTTCTCCCCTGAGTTTGTGGACTTCACTAGCC
  AGTGCCTAAGGAAGAACCCTGCAGAGCGCATGAGCTACCTGGAGCTGATGGAACACCCATTCTTCACCTT
  GCACAAAACTAAGAAGACAGACATTGCTGCCTTTGTGAAGGAGATCCTGGGAGAGGATTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225898 representing NM_008928
  Red=Cloning site Green=Tags(s)
  
  MESPAASPPASLPQTKGKSKRKKDLRISCVSKPPVSNPTPPRNLDSRTFITIGDRNFEVEADDLVTISEL
  GRGAYGVVEKVRHAQSGTIMAVKRIRATVNTQEQKRLLMDLDINMRTVDCFYTVTFYGALFREGDVWICM
  ELMDTSLDKFYRKVLEKNMKIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFG
  ISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQ
  VVEEPSPQLPADQFSPEFVDFTSQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008928
• ORF Size: 1041 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_008928.4, NP_032954.1
• RefSeq Size: 2166
• RefSeq ORF: 1044
• Locus ID: 26397