Ncbp1 (NM_001033201) Mouse Tagged ORF Clone

CAT#: MG225937

Ncbp1 (GFP-tagged) - Mouse nuclear cap binding protein subunit 1 (Ncbp1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ncbp1
• Synonyms: AU014645; AW538051; CBP80
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225937 representing NM_001033201
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGGAGGCGGCACAGCTACGAGAACGATGGTGGACAACCTCACAAAAGGAGGAAGACGTCTGATG
  CAAATGAAACTGAAGATCATTTGGAATCTTTAATATGCAAAGTAGGGGAAAAGAGTGCCTGTTCTTTAGA
  GAGCAATCTCGAAGGCCTGGCTGGTGTTTTAGAAGCTGATCTTCCTAACTACAAGAGCAAGATCTTAAGG
  CTCCTTTGTACAGTTGCACGTCTGTTACCTGAGAAGCTAACAATTTACACAACATTAGTTGGACTGCTGA
  ATGCCAGGAATTACAACTTTGGAGGAGAATTTGTAGAAGCTATGATTCGTCAACTTAAAGAATCACTAAA
  AGCAAATAACTATAATGAAGCTGTATATTTGGTCCGTTTTCTGTCTGATCTTGTGAATTGTCATGTGATC
  GCTGCCCCGTCTATGGTTGCTATGTTTGAGAATTTTGTAAGTGTAACTCAGGAAGAAGATGTGCCTCAAG
  TGCGACGGGATTGGTATGTGTATGCGTTTCTGTCATCCTTGCCCTGGGTTGGAAAGGAGTTGTATGAAAA
  AAAAGATGCAGAAATGGACCGAATCTTCTCCACCACTGAAAGCTATCTCAAAAGACGCCAGAAGACTCAC
  GTGCCCATGTTACAAGTCTGGACTGCCGACAAACCACATCCACAGGAAGAGTATTTAGATTGCCTGTGGG
  CCCAGATTCAGAAATTGAAAAAAGACCGGTGGCAGGAGAGGCACATCCTAAGACCCTACCTTGCCTTTGA
  CAGCATCCTCTGTGAAGCTCTGCAGCACAACCTGCCTCCTTTCACACCGCCGCCCCACACAGAGGACTCC
  GTGTACCCGATGCCCAGGGTCATCTTCAGGATGTTTGACTACACAGACGACCCAGAAGGCCCTGTTATGC
  CAGGGAGCCATTCGGTGGAGAGGTTTGTCATCGAGGAGAATCTTCACTGCATCATCAAGTCCTACTGGAA
  GGAAAGGAAGACTTGCGCTGCCCAGCTGGTGAGCTATCCAGGGAAGAACAAGATCCCCTTGAACTACCAC
  ATAGTGGAGGTGATCTTTGCAGAGCTGTTTCAGCTTCCAGCGCCTCCTCACATTGACGTGATGTACACTA
  CACTGCTAATCGAACTGTGCAAACTTCAGCCGGGCTCGCTGCCCCAAGTGCTTGCGCAGGCGACTGAGAT
  GCTGTACATGCGTCTGGACACGATGAGTACCACGTGTGTTGACAGGTTTATTAATTGGTTTTCTCATCAT
  CTAAGTAATTTTCAATTCCGTTGGAGCTGGGAAGATTGGTCAGATTGTCTTACTCAGGATCTTGAAAGTC
  CCAAACCAAAGTTTGTAAGGGAAGTTCTAGAGAAGTGCATGAGGTTGTCTTACCATCAACATATATTAGA
  TATTGTTCCTCCCACCTTCTCAGCTCTGTGTCCTGCAAACCCAACCTGCATTTACAAGTATGGAGATGAA
  AGTAGCAATTCTCTTCCTGGACATTCCGTGGCACTCTGTTTATCTGTTGCTTTTAAAAGTAAGGCAACCA
  ACGATGAGATCTTCAGCATTCTGAAGGACGTACCAAACCCTAACCAGGTTGATGATGATGATGAAGGGTT
  CAGATTTAACCCTTTGAAAATAGAGGTCTTTGTCCAGACTCTGCTGCACTTAGCTGCCAAGTCCTTCAGT
  CACTCCTTCAGTGCTCTTGCAAAGTTTCATGAAGTCTTCAAAACTCTGGCGGAGAGTGATAAGGGGAAGC
  TGCACGTGCTGAGAGTCATGTTTGAAGTGTGGAGGAACCATCCACAGATGATTGCTGTGCTGGTTGATAA
  GATGATTCGTACGCAGATTGTCGACTGTGCAGCAGTAGCAAACTGGATCTTCTCATCGGAGCTGTCTCGG
  GACTTTACGAGGTTGTTTGTGTGGGAAATTTTGCACTCTACAATTCGTAAGATGAACAAACATGTTCTGA
  AGATCCAGAAAGAGCTAGAAGAAGCTAAAGAGAAACTGGCGAGGCAGCACAAACGACGCAGTGATGACGA
  CGACAGGAGCAGTGACAGGAAGGACGGTGCCCTGGAGGAGCAAATAGAAAGGCTGCAGGAGAAGGTGGAG
  GCTGCTCAGAGTGAGCAGAAGAACCTCTTCCTCGTCATCTTCCAGCGTTTCATCATGATCTTGACCGAGC
  ACTTGGTACGATGTGAAACGGATGGGACCAGTATATTGACCCCGTGGTATAAGAACTGCATAGAGAGGCT
  GCAGCAGATCTTCCTACAGCATCACCAGACCATCCAGCAGTACATGGTGACCCTGGAGAACCTGCTCTTC
  ACGGCCGAGTTAGACCCTCACATCCTGGCTGTGTTCCAGCAATTCTGTGCTCTGCAGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225937 representing NM_001033201
  Red=Cloning site Green=Tags(s)
  
  MSRRRHSYENDGGQPHKRRKTSDANETEDHLESLICKVGEKSACSLESNLEGLAGVLEADLPNYKSKILR
  LLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVI
  AAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFSTTESYLKRRQKTH
  VPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDS
  VYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSYWKERKTCAAQLVSYPGKNKIPLNYH
  IVEVIFAELFQLPAPPHIDVMYTTLLIELCKLQPGSLPQVLAQATEMLYMRLDTMSTTCVDRFINWFSHH
  LSNFQFRWSWEDWSDCLTQDLESPKPKFVREVLEKCMRLSYHQHILDIVPPTFSALCPANPTCIYKYGDE
  SSNSLPGHSVALCLSVAFKSKATNDEIFSILKDVPNPNQVDDDDEGFRFNPLKIEVFVQTLLHLAAKSFS
  HSFSALAKFHEVFKTLAESDKGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAVANWIFSSELSR
  DFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDRSSDRKDGALEEQIERLQEKVE
  AAQSEQKNLFLVIFQRFIMILTEHLVRCETDGTSILTPWYKNCIERLQQIFLQHHQTIQQYMVTLENLLF
  TAELDPHILAVFQQFCALQA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033201
• ORF Size: 2370 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033201.3, NP_001028373.2
• RefSeq Size: 3167 bp
• RefSeq ORF: 2373 bp
• Locus ID: 433702
• Cytogenetics: 4 24.49 cM